Glycation promotes pulp calcification in Type 2 diabetes rat model
Aoi Takashima
Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, Suita, Japan
Contribution: Writing - original draft, Resources, Data curation, Methodology, Investigation, Visualization
Search for more papers by this authorCorresponding Author
Jiro Miura
Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, Suita, Japan
Correspondence
Jiro Miura, Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
Email: [email protected] and [email protected]
Contribution: Conceptualization, Methodology, Data curation, Investigation, Visualization, Writing - original draft, Funding acquisition, Project administration, Supervision, Writing - review & editing, Formal analysis
Search for more papers by this authorKeita Sugiyama
Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, Suita, Japan
Contribution: Methodology, Investigation, Data curation, Writing - original draft
Search for more papers by this authorMasato Shimizu
Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, Suita, Japan
Contribution: Data curation, Methodology, Investigation, Writing - original draft
Search for more papers by this authorMisa Okada
Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, Suita, Japan
Contribution: Investigation, Funding acquisition, Data curation, Resources
Search for more papers by this authorTomohiro Otani
Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Japan
Contribution: Writing - original draft, Investigation, Methodology, Data curation, Conceptualization, Project administration, Visualization
Search for more papers by this authorTadashi Nagashima
Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, Suita, Japan
Contribution: Conceptualization, Validation, Investigation
Search for more papers by this authorTetsuya Tsuda
Department of Materials Science, Graduate School of Science and Engineering, Chiba University, Chiba, Japan
Contribution: Data curation, Software, Supervision, Resources, Writing - original draft
Search for more papers by this authorTsutomu Araki
Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Japan
Contribution: Conceptualization, Supervision, Visualization, Writing - original draft, Writing - review & editing, Formal analysis
Search for more papers by this authorAoi Takashima
Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, Suita, Japan
Contribution: Writing - original draft, Resources, Data curation, Methodology, Investigation, Visualization
Search for more papers by this authorCorresponding Author
Jiro Miura
Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, Suita, Japan
Correspondence
Jiro Miura, Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
Email: [email protected] and [email protected]
Contribution: Conceptualization, Methodology, Data curation, Investigation, Visualization, Writing - original draft, Funding acquisition, Project administration, Supervision, Writing - review & editing, Formal analysis
Search for more papers by this authorKeita Sugiyama
Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, Suita, Japan
Contribution: Methodology, Investigation, Data curation, Writing - original draft
Search for more papers by this authorMasato Shimizu
Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, Suita, Japan
Contribution: Data curation, Methodology, Investigation, Writing - original draft
Search for more papers by this authorMisa Okada
Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, Suita, Japan
Contribution: Investigation, Funding acquisition, Data curation, Resources
Search for more papers by this authorTomohiro Otani
Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Japan
Contribution: Writing - original draft, Investigation, Methodology, Data curation, Conceptualization, Project administration, Visualization
Search for more papers by this authorTadashi Nagashima
Division for Interdisciplinary Dentistry, Graduate School of Dentistry, Osaka University, Suita, Japan
Contribution: Conceptualization, Validation, Investigation
Search for more papers by this authorTetsuya Tsuda
Department of Materials Science, Graduate School of Science and Engineering, Chiba University, Chiba, Japan
Contribution: Data curation, Software, Supervision, Resources, Writing - original draft
Search for more papers by this authorTsutomu Araki
Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Japan
Contribution: Conceptualization, Supervision, Visualization, Writing - original draft, Writing - review & editing, Formal analysis
Search for more papers by this authorAoi Takashima, Jiro Miura are joint first authors and contributed equally to this study.
Abstract
Objectives
Intrapulpal calcifications can occur in the dental pulp of patients with diabetes. We focused on the association between ectopic calcifications in the dental pulp and advanced glycation end products (AGEs) in Spontaneously Diabetic Torii (SDT)-fatty rats, an obese type 2 diabetic rat model, to determine the mechanism of calcification with pulp stone in the dental pulp.
Materials and Methods
Pathologic calcification in the dental pulp of SDT-fatty rats was observed using electron microscopy and immunohistochemical analysis. Moreover, mechanical analysis of periapical region of molar tooth against occlusal force was performed.
Results
In SDT-fatty rats, pathogenic pulpal calcifications occurred during blood glucose elevation after 6 weeks, and granular calcification was observed in the dental pulp after 11 weeks. Pentosidine, a major AGE, and the receptor for AGEs were strongly expressed in the dental pulp of SDT-fatty rats. S100A8, TNF-α, and IL-6 also showed positive response in the dental pulp of the SDT-fatty rat, which indicated pulpal inflammation. Blood flow disorder and hypoxic dental pulp cells were also observed. In silico simulation, strain from occlusal force concentrates on the root apex.
Conclusions
Glycation makes blood vessels fragile, and occlusal forces damage the vessels mechanically. These are factors for intrapulpal calcification of diabetes.
CONFLICT OF INTEREST STATEMENT
The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are openly available in Osaka University Knowledge Archive at https://ir.library.osaka-u.ac.jp/repo/ouka/all/?lang=1.
Supporting Information
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| odi14529-sup-0001-Supinfo.tifimage/tif, 970.8 KB |
Figure S1. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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