ORIGINAL ARTICLE

LncRNA MAFG-AS1 regulates human periodontal ligament stem cell proliferation and Toll-like receptor 4 expression

Kaixin Wangzhou

School of Management, Hainan Medical University, Haikou, Hainan, China

Lei Gong

Department of Stomatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan, China

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Cheng Liu,

Cheng Liu

Department of Stomatology, Harbin Stomatological Hospital, Harbin, Heilongjiang, China

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Yi Tan,

Yi Tan

Department of Stomatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan, China

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Jingxin Chen,

Jingxin Chen

Department of Stomatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan, China

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Conghui Li,

Conghui Li

Department of Stomatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan, China

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Zhiying Lai,

Zhiying Lai

Department of Stomatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan, China

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Chunbo Hao,

Corresponding Author

Chunbo Hao

[email protected]

orcid.org/0000-0003-2747-4196

Department of Stomatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan, China

Correspondence

Chunbo Hao, Department of Stomatology, Hainan Province People's Hospital, No. 19 Xiuhua Road, Xiuying District, Haikou City, Hainan Province 570011, China.

Email [email protected]

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Kaixin Wangzhou,

Kaixin Wangzhou

School of Management, Hainan Medical University, Haikou, Hainan, China

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Lei Gong,

Lei Gong

Department of Stomatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan, China

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Cheng Liu,

Cheng Liu

Department of Stomatology, Harbin Stomatological Hospital, Harbin, Heilongjiang, China

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Yi Tan,

Yi Tan

Department of Stomatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan, China

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Jingxin Chen,

Jingxin Chen

Department of Stomatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan, China

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Conghui Li,

Conghui Li

Department of Stomatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan, China

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Zhiying Lai,

Zhiying Lai

Department of Stomatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan, China

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Chunbo Hao,

Corresponding Author

Chunbo Hao

[email protected]

orcid.org/0000-0003-2747-4196

Department of Stomatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan, China

Correspondence

Chunbo Hao, Department of Stomatology, Hainan Province People's Hospital, No. 19 Xiuhua Road, Xiuying District, Haikou City, Hainan Province 570011, China.

Email [email protected]

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First published: 16 March 2020

https://doi.org/10.1111/odi.13330

Citations: 15

Wangzhou and Gong contributed equally to this work.

Funding information

This work was supported by Key Research and Development Project of Hainan (ZDYF2019216); Key Scientific Research Projects of Higher Education in Hainan Province（Hnky2019ZD-22）; Hainan Natural Science Foundation (817320).

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Abstract

LncRNA MAFG-AS1 is predicted to interact with miR-146a, which can target Toll-like receptor 4 (TLR4), a key player in periodontitis. This study aimed to investigate the roles of MAFG-AS1 in periodontitis. It was observed that MAFG-AS1 was downregulated in the human periodontal ligament stem cells (PDLSCs) derived from periodontitis-affected teeth. Dual-luciferase assay revealed that co-transfection of MAFG-AS1 expression vector and miR-146a mimic showed significantly lower relative luciferase activity comparing to co-transfection of MAFG-AS1 expression vector and negative control (NC) miRNA. However, MAFG-AS1 and miR-146a failed to affect each other. Interestingly, MAFG-AS1 overexpression led to the upregulated TLR4. In addition, MAFG-AS1 overexpression also led to the inhibited proliferation of PDLSCs. Therefore, MAFG-AS1 may regulate the proliferation of PDLSCs and the expression of TLR4 to participate in periodontitis.

CONFLICT OF INTERESTS

The authors declare that they have no competing interests.

Open Research

DATA AVAILABILITY STATEMENT

The analyzed data sets generated during the study are available from the corresponding author on reasonable request.

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Volume26, Issue6

September 2020

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LncRNA MAFG-AS1 regulates human periodontal ligament stem cell proliferation and Toll-like receptor 4 expression

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LncRNA MAFG-AS1 regulates human periodontal ligament stem cell proliferation and Toll-like receptor 4 expression

Abstract

CONFLICT OF INTERESTS

Open Research

DATA AVAILABILITY STATEMENT

REFERENCES

Citing Literature

References

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