Mitochondrial polymorphisms and dysfunction related to aggressive periodontitis: a pilot study
X Wang
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China
Search for more papers by this authorCorresponding Author
Q Luan
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China
Correspondence: Qingxian Luan, Department of Periodontology, Peking University School and Hospital of Stomatology, 22 Zhongguancun Nandajie, Haidian District, Beijing 100081, China. Tel: +8610 82195497, Fax: +8610 62173402, E-mail: [email protected]Search for more papers by this authorQ Chen
State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Chaoyang District, Beijing, China
Search for more papers by this authorL Zhao
State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Chaoyang District, Beijing, China
Search for more papers by this authorY Guo
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China
Search for more papers by this authorX Wang
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China
Search for more papers by this authorCorresponding Author
Q Luan
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China
Correspondence: Qingxian Luan, Department of Periodontology, Peking University School and Hospital of Stomatology, 22 Zhongguancun Nandajie, Haidian District, Beijing 100081, China. Tel: +8610 82195497, Fax: +8610 62173402, E-mail: [email protected]Search for more papers by this authorQ Chen
State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Chaoyang District, Beijing, China
Search for more papers by this authorL Zhao
State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Chaoyang District, Beijing, China
Search for more papers by this authorY Guo
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China
Search for more papers by this authorAbstract
Aim
To investigate whether aggressive periodontitis (AgP) is associated with specific mtDNA polymorphisms or point mutations and furthermore whether mitochondrial dysfunction occurs in gingival fibroblasts of AgP patients.
Materials and methods
The mitochondrial DNA coding regions were amplified and sequenced in 22 overlapped fragments in 34 patients with AgP and 28 healthy controls for initial screening. We selected eleven SNPs for detailed investigation in the rest 30 AgP patients and 26 healthy controls, because all other variants occurred at relatively low frequencies or had no difference between two groups. Logistic regression models were used to analyze the association between mtDNA variants and AgP. Gingival fibroblasts were cultured from four patients with AgP and four healthy controls, and then mitochondrial membrane potential, reactive oxygen species production and cell proliferation were analyzed.
Results
Significant association was observed between aggressive periodontitis and eight mitochondrial polymorphisms: ‘8701A-9540T-10400C-10873T-14783T-15043G-15301G’ (OR = 3.471 (1.610–7.483), P = 0.001) and 10398A (OR = 3.238 (1.481–7.078), P = 0.003). Compared with the controls, patients with aggressive periodontitis had a decrease in mitochondrial membrane potential and an increase in reactive oxygen species production.
Conclusion
In conclusion, we propose that mitochondrial dysfunction and ‘8701A-9540T-10400C-10873T-14783T-15043G-15301G, 10398A’ are associated with and may increase the susceptibility to AgP in the Han Chinese population.
Supporting Information
| Filename | Description |
|---|---|
| odi12163-sup-0001-TableS1.docWord document, 756 KB | Table S1 A total of 421 variants found in the study after initial screening. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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