Platelet increases survival in a model of 90% hepatectomy in rats
Mónica L. López
Gene Therapy Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, do Rio Grande do Sul, Brazil
Post-Graduation Program on Genetics and Molecular Biology, Universidade Federal do Rio Grande do Sul, Porto Alegre, do Rio Grande do Sul, Brazil
Search for more papers by this authorCarlos O. Kieling
Post-Graduation Program in Medicine: Sciences in Gastroenterology, Universidade Federal do Rio Grande do Sul, Porto Alegre, do Rio Grande do Sul, Brazil
Experimental Hepatology Laboratory, Hospital de Clínicas de Porto Alegre, Porto Alegre, do Rio Grande do Sul, Brazil
Search for more papers by this authorCarolina Uribe Cruz
Gene Therapy Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, do Rio Grande do Sul, Brazil
Post-Graduation Program on Genetics and Molecular Biology, Universidade Federal do Rio Grande do Sul, Porto Alegre, do Rio Grande do Sul, Brazil
Search for more papers by this authorCorresponding Author
Alessandro Osvaldt
Post-Graduation Program in Surgery, Universidade Federal do Rio Grande do Sul, Porto Alegre, do Rio Grande do Sul, Brazil
Correspondence
Alessandro Osvaldt, Medical School of Universidade Federal do Rio Grande do Sul, Porto Alegre, Rua Ramiro Barcelos 2350, Brazil
Tel: +55 51 3028 1828
Fax: +55 51 3028 1828
e-mail: [email protected]
Search for more papers by this authorGustavo Ochs de Muñoz
Gene Therapy Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, do Rio Grande do Sul, Brazil
Search for more papers by this authorLuise Meurer
Post-Graduation Program in Medicine: Sciences in Gastroenterology, Universidade Federal do Rio Grande do Sul, Porto Alegre, do Rio Grande do Sul, Brazil
Experimental Pathology Unit, Hospital de Clínicas de Porto Alegre, Porto Alegre, do Rio Grande do Sul, Brazil
Search for more papers by this authorLucia Silla
Center for Cell Technology, Hospital de Clínicas de Porto Alegre, Porto Alegre, do Rio Grande do Sul, Brazil
Search for more papers by this authorUrsula Matte
Gene Therapy Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, do Rio Grande do Sul, Brazil
Post-Graduation Program on Genetics and Molecular Biology, Universidade Federal do Rio Grande do Sul, Porto Alegre, do Rio Grande do Sul, Brazil
Search for more papers by this authorMónica L. López
Gene Therapy Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, do Rio Grande do Sul, Brazil
Post-Graduation Program on Genetics and Molecular Biology, Universidade Federal do Rio Grande do Sul, Porto Alegre, do Rio Grande do Sul, Brazil
Search for more papers by this authorCarlos O. Kieling
Post-Graduation Program in Medicine: Sciences in Gastroenterology, Universidade Federal do Rio Grande do Sul, Porto Alegre, do Rio Grande do Sul, Brazil
Experimental Hepatology Laboratory, Hospital de Clínicas de Porto Alegre, Porto Alegre, do Rio Grande do Sul, Brazil
Search for more papers by this authorCarolina Uribe Cruz
Gene Therapy Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, do Rio Grande do Sul, Brazil
Post-Graduation Program on Genetics and Molecular Biology, Universidade Federal do Rio Grande do Sul, Porto Alegre, do Rio Grande do Sul, Brazil
Search for more papers by this authorCorresponding Author
Alessandro Osvaldt
Post-Graduation Program in Surgery, Universidade Federal do Rio Grande do Sul, Porto Alegre, do Rio Grande do Sul, Brazil
Correspondence
Alessandro Osvaldt, Medical School of Universidade Federal do Rio Grande do Sul, Porto Alegre, Rua Ramiro Barcelos 2350, Brazil
Tel: +55 51 3028 1828
Fax: +55 51 3028 1828
e-mail: [email protected]
Search for more papers by this authorGustavo Ochs de Muñoz
Gene Therapy Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, do Rio Grande do Sul, Brazil
Search for more papers by this authorLuise Meurer
Post-Graduation Program in Medicine: Sciences in Gastroenterology, Universidade Federal do Rio Grande do Sul, Porto Alegre, do Rio Grande do Sul, Brazil
Experimental Pathology Unit, Hospital de Clínicas de Porto Alegre, Porto Alegre, do Rio Grande do Sul, Brazil
Search for more papers by this authorLucia Silla
Center for Cell Technology, Hospital de Clínicas de Porto Alegre, Porto Alegre, do Rio Grande do Sul, Brazil
Search for more papers by this authorUrsula Matte
Gene Therapy Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, do Rio Grande do Sul, Brazil
Post-Graduation Program on Genetics and Molecular Biology, Universidade Federal do Rio Grande do Sul, Porto Alegre, do Rio Grande do Sul, Brazil
Search for more papers by this authorAbstract
Background & Aims
Ninety per cent hepatectomy in rodents is a model for acute liver failure. It has been reported that platelets have a strong effect enhancing liver regeneration, because of the production of several growth factors such as serotonin. The aim of this study was to investigate the role of microencapsulated platelets on 90% hepatectomy in rats.
Methods
Platelets (PLT) were microencapsulated in sodium alginate and implanted in the peritoneum of rats after 90% partial hepatectomy (PH). Control group received empty capsules (EC). Animals were euthanized at 6, 12, 24, 48 and 72 h post PH (n = 9–12/group/time) to evaluate liver regeneration rate, mitotic index, liver content, serum and tissue levels of Interleukin 6 (IL-6) and serotonin and its receptor 5-hydroxytryptamine type 2B (5Ht2b). Survival rate in 10 days was evaluated in a different set of animals (n = 20/group).
Results
Platelets group showed the highest survival rate despite the lowest liver regeneration rate at any time point. Mitotic and BrdU index showed no difference between groups. However, the number of hepatocytes was higher and the internuclear distance was shorter for PLT group. Liver dry weight was similar in both groups indicating that water was the main responsible factor for the weight difference. Gene expression of IL-6 in the liver was significantly higher in EC group 6 h after PH, whereas 5Ht2b was up-regulated at 72 h in PLT group.
Conclusions
Platelets enhance survival of animals with 90% PH, probably by an early protective effect on hepatocytes and the increase in growth factor receptors.
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