Coenzyme Q in pregnant women and rats with intrahepatic cholestasis
Manuela R. Martinefski
Analytical Chemistry, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina
Both authors contributed equally to the study.Search for more papers by this authorMario D. Contin
Analytical Chemistry, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina
Both authors contributed equally to the study.Search for more papers by this authorMyrian R. Rodriguez
Pathophysiology-INIGEM, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Tecnológicas, CONICET, Buenos Aires, Argentina
Search for more papers by this authorEstefanía M. Geréz
Physical Chemistry-IBIMOL, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina
Search for more papers by this authorMónica L. Galleano
Physical Chemistry-IBIMOL, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Tecnológicas, CONICET, Buenos Aires, Argentina
Search for more papers by this authorSilvia E. Lucangioli
Drug Quality, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Tecnológicas, CONICET, Buenos Aires, Argentina
Search for more papers by this authorLiliana G. Bianciotti
Pathophysiology-INIGEM, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Tecnológicas, CONICET, Buenos Aires, Argentina
Search for more papers by this authorCorresponding Author
Valeria P. Tripodi
Drug Quality, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Tecnológicas, CONICET, Buenos Aires, Argentina
Correspondence
Professor Dr Valeria P. Tripodi, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Junin 956, 5th floor, Buenos Aires 1113, Argentina
Tel/Fax: +54 11 4964 8200 (int. 8267)
e-mail: [email protected]
Search for more papers by this authorManuela R. Martinefski
Analytical Chemistry, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina
Both authors contributed equally to the study.Search for more papers by this authorMario D. Contin
Analytical Chemistry, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina
Both authors contributed equally to the study.Search for more papers by this authorMyrian R. Rodriguez
Pathophysiology-INIGEM, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Tecnológicas, CONICET, Buenos Aires, Argentina
Search for more papers by this authorEstefanía M. Geréz
Physical Chemistry-IBIMOL, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina
Search for more papers by this authorMónica L. Galleano
Physical Chemistry-IBIMOL, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Tecnológicas, CONICET, Buenos Aires, Argentina
Search for more papers by this authorSilvia E. Lucangioli
Drug Quality, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Tecnológicas, CONICET, Buenos Aires, Argentina
Search for more papers by this authorLiliana G. Bianciotti
Pathophysiology-INIGEM, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Tecnológicas, CONICET, Buenos Aires, Argentina
Search for more papers by this authorCorresponding Author
Valeria P. Tripodi
Drug Quality, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Tecnológicas, CONICET, Buenos Aires, Argentina
Correspondence
Professor Dr Valeria P. Tripodi, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Junin 956, 5th floor, Buenos Aires 1113, Argentina
Tel/Fax: +54 11 4964 8200 (int. 8267)
e-mail: [email protected]
Search for more papers by this authorAbstract
Background & Aims
Intrahepatic cholestasis of pregnancy is a high-risk liver disease given the eventual deleterious consequences that may occur in the foetus. It is accepted that the abnormal accumulation of hydrophobic bile acids in maternal serum are responsible for the disease development. Hydrophobic bile acids induce oxidative stress and apoptosis leading to the damage of the hepatic parenchyma and eventually extrahepatic tissues. As coenzyme Q (CoQ) is considered an early marker of oxidative stress in this study, we sought to assess CoQ levels, bile acid profile and oxidative stress status in intrahepatic cholestasis.
Methods
CoQ, vitamin E and malondialdehyde were measured in plasma and/or tissues by HPLC-UV method whereas serum bile acids by capillary electrophoresis in rats with ethinyl estradiol-induced cholestasis and women with pregnancy cholestasis.
Results
CoQ and vitamin E plasma levels were diminished in both rats and women with intrahepatic cholestasis. Furthermore, reduced CoQ was also found in muscle and brain of cholestatic rats but no changes were observed in heart or liver. In addition, a positive correlation between CoQ and ursodeoxycholic/lithocholic acid ratio was found in intrahepatic cholestasis suggesting that increased plasma lithocholic acid may be intimately related to CoQ depletion in blood and tissues.
Conclusion
Significant CoQ and vitamin E depletion occur in both animals and humans with intrahepatic cholestasis likely as the result of increased hydrophobic bile acids known to produce significant oxidative stress. Present findings further suggest that antioxidant supplementation complementary to traditional treatment may improve cholestasis outcome.
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