Effects of oleanolic acid acetate on bone formation in an experimental periodontitis model in mice
Nirpesh Adhikari
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorSanjiv Neupane
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorYam Prasad Aryal
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorMi Choi
Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorWern-Joo Sohn
Pre-Major of Cosmetics and Pharmaceutics, Daegu Haany University, Gyeongsan, Korea
Search for more papers by this authorYoungkyun Lee
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorJae-Kwang Jung
Department of Oral Medicine, School of Dentistry, IHBR, Kyungpook National University, Daegu, South Korea
Search for more papers by this authorJung-Hong Ha
Department of Conservative Dentistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorSo-Young Choi
Department of Oral and Maxillofacial Surgery, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorJo-Young Suh
Department of Periodontology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorJi-Youn Kim
Department of Dental Hygiene, College of Health Science, Gachon University, Incheon, Korea
Search for more papers by this authorMun-Chual Rho
Immunoregulatory Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si, Korea
Search for more papers by this authorTae-Hoon Lee
Department of Oral Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju, Korea
Search for more papers by this authorHitoshi Yamamoto
Department of Histology and Developmental Biology, Tokyo Dental College, Tokyo, Japan
Search for more papers by this authorChang-Hyeon An
Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorSang-Hyun Kim
Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu, Korea
Search for more papers by this authorCorresponding Author
Seo-Young An
Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Correspondence
Jae-Young Kim, Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea.
Email: [email protected]
and
Seo-Young An, Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Jae-Young Kim
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Correspondence
Jae-Young Kim, Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea.
Email: [email protected]
and
Seo-Young An, Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea.
Email: [email protected]
Search for more papers by this authorNirpesh Adhikari
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorSanjiv Neupane
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorYam Prasad Aryal
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorMi Choi
Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorWern-Joo Sohn
Pre-Major of Cosmetics and Pharmaceutics, Daegu Haany University, Gyeongsan, Korea
Search for more papers by this authorYoungkyun Lee
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorJae-Kwang Jung
Department of Oral Medicine, School of Dentistry, IHBR, Kyungpook National University, Daegu, South Korea
Search for more papers by this authorJung-Hong Ha
Department of Conservative Dentistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorSo-Young Choi
Department of Oral and Maxillofacial Surgery, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorJo-Young Suh
Department of Periodontology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorJi-Youn Kim
Department of Dental Hygiene, College of Health Science, Gachon University, Incheon, Korea
Search for more papers by this authorMun-Chual Rho
Immunoregulatory Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si, Korea
Search for more papers by this authorTae-Hoon Lee
Department of Oral Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju, Korea
Search for more papers by this authorHitoshi Yamamoto
Department of Histology and Developmental Biology, Tokyo Dental College, Tokyo, Japan
Search for more papers by this authorChang-Hyeon An
Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Search for more papers by this authorSang-Hyun Kim
Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu, Korea
Search for more papers by this authorCorresponding Author
Seo-Young An
Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Correspondence
Jae-Young Kim, Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea.
Email: [email protected]
and
Seo-Young An, Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Jae-Young Kim
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea
Correspondence
Jae-Young Kim, Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea.
Email: [email protected]
and
Seo-Young An, Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea.
Email: [email protected]
Search for more papers by this authorAbstract
Objective
We evaluated the role of oleanolic acid acetate (OAA), a triterpenoid commonly used in the treatment of liver disorders, inflammatory diseases, and metastasis, in bone formation after tooth loss by periodontitis.
Background
Periodontitis causes the sequential degradation of the alveolar bone and associated structures, resulting in tooth loss. Several studies have attempted to regenerate the bone for implantation following tooth loss.
Methods
Maxillary left second molar was extracted from 8-week-old male mice following induction of periodontitis by ligature for 5 days. The extraction socket was treated with 50 ng/µL OAA for 1, 2, and 3 weeks. Detailed morphological changes were examined using Masson's trichrome staining, and the precise localization patterns of various signaling molecules, including CD31, F4/80, interleukin (IL)-6, and osteocalcin, were observed. The volume of bone formation was examined by Micro-CT. Osteoclasts were enumerated using tartrate-resistant acid phosphatase (TRAP) staining. For molecular dissection of signaling molecules, we employed the hanging-drop in vitro cultivation method at E14 for 1 day and examined the expression pattern of transforming growth factor (TGF)-β superfamily and Wnt signaling genes.
Results
Histomorphometrical examinations showed facilitated bone formation in the extraction socket following OAA treatment. In addition, OAA-treated specimens showed the altered localization patterns of inflammatory and bone formation-related signaling molecules including CD31, F4/80, IL-6, and osteocalcin. Also, embryonic tooth germ mesenchymal tissue cultivation with OAA treatment showed the significant altered expression patterns of signaling molecules such as transforming growth factor (TGF)-β superfamily and Wnt signaling.
Conclusions
Oleanolic acid acetate induces bone formation and remodeling through proper modulation of osteoblast, osteoclast, and inflammation with regulations of TGF-β and Wnt signaling.
CONFLICT OF INTEREST
The authors declare no conflicts of interests.
Supporting Information
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