Original Article
Characterization of γδT cells in naïve and HIV-infected chimpanzees and their responses to T-cell activators in vitro
Vida L. Hodara,
Laura M. Parodi,
Deborah Chavez,
Lisa M. Smith,
Robert Lanford,
Luis D. Giavedoni,
Corresponding Author
Vida L. Hodara
Department of Virology & Immunology, Texas Biomedical Research Institute, San Antonio, TX, USA
Southwest National Primate Research Center, San Antonio, TX, USA
Correspondence
Vida L. Hodara, PhD, Department of Virology & Immunology, Texas Biomedical Research Institute, 7620 NW Loop 410, San Antonio, TX 78227, USA.
Tel.: (210) 258 9683;
fax: (210) 670 3329;
e-mail: [email protected]
Search for more papers by this authorLaura M. Parodi
Department of Virology & Immunology, Texas Biomedical Research Institute, San Antonio, TX, USA
Search for more papers by this authorDeborah Chavez
Department of Virology & Immunology, Texas Biomedical Research Institute, San Antonio, TX, USA
Search for more papers by this authorLisa M. Smith
Department of Virology & Immunology, Texas Biomedical Research Institute, San Antonio, TX, USA
Search for more papers by this authorRobert Lanford
Department of Virology & Immunology, Texas Biomedical Research Institute, San Antonio, TX, USA
Southwest National Primate Research Center, San Antonio, TX, USA
Search for more papers by this authorLuis D. Giavedoni
Department of Virology & Immunology, Texas Biomedical Research Institute, San Antonio, TX, USA
Southwest National Primate Research Center, San Antonio, TX, USA
Search for more papers by this authorVida L. Hodara,
Laura M. Parodi,
Deborah Chavez,
Lisa M. Smith,
Robert Lanford,
Luis D. Giavedoni,
Corresponding Author
Vida L. Hodara
Department of Virology & Immunology, Texas Biomedical Research Institute, San Antonio, TX, USA
Southwest National Primate Research Center, San Antonio, TX, USA
Correspondence
Vida L. Hodara, PhD, Department of Virology & Immunology, Texas Biomedical Research Institute, 7620 NW Loop 410, San Antonio, TX 78227, USA.
Tel.: (210) 258 9683;
fax: (210) 670 3329;
e-mail: [email protected]
Search for more papers by this authorLaura M. Parodi
Department of Virology & Immunology, Texas Biomedical Research Institute, San Antonio, TX, USA
Search for more papers by this authorDeborah Chavez
Department of Virology & Immunology, Texas Biomedical Research Institute, San Antonio, TX, USA
Search for more papers by this authorLisa M. Smith
Department of Virology & Immunology, Texas Biomedical Research Institute, San Antonio, TX, USA
Search for more papers by this authorRobert Lanford
Department of Virology & Immunology, Texas Biomedical Research Institute, San Antonio, TX, USA
Southwest National Primate Research Center, San Antonio, TX, USA
Search for more papers by this authorLuis D. Giavedoni
Department of Virology & Immunology, Texas Biomedical Research Institute, San Antonio, TX, USA
Southwest National Primate Research Center, San Antonio, TX, USA
Search for more papers by this authorAbstract
Background
γδT cells are effector cells that eliminate cancer and virus-infected cells. Chimpanzees are an endangered species that can naturally and experimentally be infected with SIV and HIV, respectively, but no information about the functionality of γδT cells during chronic lentiviral infection is currently available.
Methods
Healthy and HIV-infected chimpanzee γδT cells were characterized by flow cytometry. γδT subsets were studied after stimulation with T-cell activators, and the release of cytokines was analyzed by Luminex assay.
Results
γδT-cell subsets, Vδ1 and Vδ2Vγ9, showed different patterns in the expression of CD4, CD195, CD159a, and CD159c. Stimulation of γδT cells resulted in increased levels of CD4 and HLA-DR, which is more pronounced in Vδ1 T cells. Distinct cytokine patterns were found between healthy and HIV-infected chimpanzees.
Conclusions
Analyses of major chimpanzee γδT subsets show similarities to human γδT cells and suggest different functionality and roles in their immune response against HIV infection.
References
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