Partial characterization and in vitro evaluation of a lytic bacteriophage for biocontrol of Campylobacter jejuni in mutton and chicken meat
Corresponding Author
Tze Young Thung
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Malaysia
Correspondence
Tze Young Thung, Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia.
Email: [email protected]
Search for more papers by this authorEpeng Lee
Food Safety and Food Integrity, Institute of Tropical Agriculture and Food Security (ITAFoS), Universiti Putra Malaysia, Serdang, Malaysia
Search for more papers by this authorNor Ainy Mahyudin
Department of Food Service Management, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Malaysia
Search for more papers by this authorChe Wan Jasimah Wan Mohamed Radzi
Department of Science and Technology Studies, Faculty of Science Building, Universiti of Malaya, Kuala Lumpur, Malaysia
Search for more papers by this authorNurzafirah Mazlan
Department of Diagnostic and Allied Science, Faculty of Health and Life Sciences, Management and Science University, Shah Alam, Malaysia
Search for more papers by this authorChia Wanq Tan
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Malaysia
Search for more papers by this authorSon Radu
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Malaysia
Food Safety and Food Integrity, Institute of Tropical Agriculture and Food Security (ITAFoS), Universiti Putra Malaysia, Serdang, Malaysia
Search for more papers by this authorCorresponding Author
Tze Young Thung
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Malaysia
Correspondence
Tze Young Thung, Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia.
Email: [email protected]
Search for more papers by this authorEpeng Lee
Food Safety and Food Integrity, Institute of Tropical Agriculture and Food Security (ITAFoS), Universiti Putra Malaysia, Serdang, Malaysia
Search for more papers by this authorNor Ainy Mahyudin
Department of Food Service Management, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Malaysia
Search for more papers by this authorChe Wan Jasimah Wan Mohamed Radzi
Department of Science and Technology Studies, Faculty of Science Building, Universiti of Malaya, Kuala Lumpur, Malaysia
Search for more papers by this authorNurzafirah Mazlan
Department of Diagnostic and Allied Science, Faculty of Health and Life Sciences, Management and Science University, Shah Alam, Malaysia
Search for more papers by this authorChia Wanq Tan
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Malaysia
Search for more papers by this authorSon Radu
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Malaysia
Food Safety and Food Integrity, Institute of Tropical Agriculture and Food Security (ITAFoS), Universiti Putra Malaysia, Serdang, Malaysia
Search for more papers by this authorFunding information: Fundamental Research Grant Scheme (FRGS) of the Ministry of Higher Education (MOHE), Malaysia, Grant/Award Number: 01-01-18-2015FR
Abstract
Campylobacter jejuni is a major foodborne pathogen causing acute bacterial enteritis worldwide. Screening of novel bacteriophages as biocontrol tools to eliminate C. jejuni in food during pre- and postharvest is an emerging research field. In this study, we partially characterized C. jejuni bacteriophage CJ01 for potential use as biocontrol agent. Transmission electron microscopy revealed bacteriophage CJ01 belonged to the Myoviridae family with a genome size of approximately 136 kb. The results of host range assay revealed CJ01 was able to infect C. jejuni and C. lari strains, with burst size about 35 phages per infected cell and latent period of 30 min. The effectiveness of bacteriophage CJ01 (with 100 multiplicity of infection = 100) was determined in mutton and chicken meat artificially contaminated with C. jejuni. The amount of C. jejuni in treated mutton and chicken meat were significantly reduced by 1.70 log CFU/g and 1.68 log CFU/g, respectively, after 48 hr stored at refrigeration temperature (4°C). The data presented here suggest that bacteriophage CJ01 can be used as a postharvest biocontrol agent.
CONFLICT OF INTEREST
All authors declare no conflicts of interest.
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