Volume 40, Issue 3 pp. 202-208

Fate and protective effect of marrow stromal cells after subretinal transplantation

Hong Pan,

Hong Pan

The Experimental Center, The First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China

College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China

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Xinjian Liu,

Xinjian Liu

College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China

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Jihong Wu,

Jihong Wu

The Experimental Center, The First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China

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Yuhua Tian,

Yuhua Tian

The Experimental Center, The First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China

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Shenghai Zhang,

Shenghai Zhang

The Experimental Center, The First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China

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Zhixin Lin,

Zhixin Lin

College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China

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Qian Huang,

Corresponding Author

Qian Huang

The Experimental Center, The First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China

*Corresponding author: Tel, 86-21-63240090; Fax, 86-21-63240825; E-mail, [email protected]Search for more papers by this author

Hong Pan,

Hong Pan

The Experimental Center, The First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China

College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China

Search for more papers by this author

Xinjian Liu,

Xinjian Liu

College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China

Search for more papers by this author

Jihong Wu,

Jihong Wu

The Experimental Center, The First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China

Search for more papers by this author

Yuhua Tian,

Yuhua Tian

The Experimental Center, The First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China

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Shenghai Zhang,

Shenghai Zhang

The Experimental Center, The First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China

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Zhixin Lin,

Zhixin Lin

College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China

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Qian Huang,

Corresponding Author

Qian Huang

The Experimental Center, The First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China

*Corresponding author: Tel, 86-21-63240090; Fax, 86-21-63240825; E-mail, [email protected]Search for more papers by this author

First published: 19 March 2008

https://doi.org/10.1111/j.1745-7270.2008.00398.x

This work was supported by the grants from the National Basic Research Project of China (2004CB518804), National Natural Science Foundation for Outstanding Youth (30325043 and 30428015), Science and Technology Committee of Shanghai (064119539), and Leading Medical Talent of Shanghai (040308)

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Abstract

Engraftment of marrow stromal cells (MSCs) has been proposed as a therapeutic approach for degenerative diseases. In this study we investigated the fate and dynamic progress of grafted MSCs in living retina with the aim of evaluating the use of transplanted MSCs to treat retinal degeneration. Approximately 1×10⁵gfp-MSCs in 2 μl phosphate-buffered saline were injected into the subretinal space of adult Sprague-Dawley rats. Two weeks later, approximately 0.174%±0.082% of the transplanted cells had survived and diffused into the subretinal space. Nine weeks after transplantation the surviving gfp-MSCs accounted for 0.049%±0.023% of the number of cells injected and were mainly located at the injection site. The same number of MSCs were transplanted into the left eye subretinal space of 3-week-old hereditary retinal degenerative Royal College of Surgeons rats, and phosphate-buffered saline was injected into their right eyes as a control. Five weeks after transplantation, the amount of rudimentary photo-receptors was more significantly increased in grafted eyes than in control eyes. The results indicated that grafted MSCs could survive and rescue retinal degeneration.

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Volume40, Issue3

March 2008

Pages 202-208

Fate and protective effect of marrow stromal cells after subretinal transplantation

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Fate and protective effect of marrow stromal cells after subretinal transplantation

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