Author for correspondence: Adair R. S. Santos, Department of Physiological Sciences, Center of Biological Sciences, Federal University of Santa Catarina, Florianópolis 88040-900, SC, Brazil (fax +55 48 37219672, e-mail [email protected]).

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Abstract

Abstract: This study investigated the role of the glutamatergic system on the antinociception caused by Polygala sabulosa hydroalcoholic extract (HE). The systems mediated by substance P, capsaicin, interleukin-1β (IL-1β) and tumour necrosis factor-α (TNF-α) were also investigated. P. sabulosa HE given orally produced a significant inhibition of glutamate-induced paw licking [ID₅₀ = 530.3 (416.7–674.8) mg/kg and inhibition of 79 ± 6% at 1000 mg/kg]. The plant derivatives α-spinasterol, scopoletin and styryl-2-pyrones (compound 1 and 3) (10 mg/kg, intraperitoneally) inhibited 80 ± 7%, 46 ± 11%, 45 ± 11% and 35 ± 13% the nociceptive response caused by glutamate, respectively. Furthermore, P. sabulosa HE (500 mg/kg, orally) caused marked inhibition of nociceptive response induced by intrathecal injection of glutamate, N-methyl-d-aspartic acid, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, kainate, TNF-α and IL-1β, with inhibitions of 44 ± 7%, 55 ± 4%, 38 ± 10%, 61 ± 7%, 76 ± 9% and 100%, respectively. In contrast, P. sabulosa HE (500 mg/kg, orally) did not affect the biting response induced by the metabotropic glutamatergic receptor agonist (±)-1-aminocyclopentane-trans-1,3-dicarboxylic acid, substance P and capsaicin. The locomotor activity was altered only in mice treated with a very high dose (1000 mg/kg) of P. sabulosa HE. Our results showed that the antinociceptive effects of P. sabulosa HE are associated with an inhibition of glutamatergic transmission and an inhibition of pathways dependent on pro-inflammatory cytokines. The plant derivatives α-spinasterol, scopoletin and styryl-2-pyrones play an important role on the antinociceptive effects of P. sabulosa HE.

References

1 Wasicky R. In: Anais da Faculdade de Farmácia e Odontologia da Universidade de São Paulo, 1945, vol. 4, pp. 189–217, São Paulo, Brazil.
Google Scholar
2 Cyong JC, Ki SM, Iijima K, Kobayashi T, Furuya M. Clinical and pharmacological studies on liver diseases treated with Kampo herbal medicine. Am J Chin Med 2000; 28: 351–60.
10.1142/S0192415X00000416
CAS PubMed Web of Science® Google Scholar
3 Pizzolatti MG, Koga AH, Grisard EC, Steindel M. Trypanocidal activity of extracts from Brazilian Atlantic Rain Forest plant species. Phytomedicine 2003; 10: 422–6.
10.1078/0944-7113-00252
CAS PubMed Web of Science® Google Scholar
4 Mak NK, Lung HL, Wong RN, Leung HW, Tsang HY, Leung KN. Expression of protein kinase C isoforms in euxanthone-induced differentiation of neuroblastoma cells. Planta Med 2001; 67: 400–5.
10.1055/s-2001-15809
CAS PubMed Web of Science® Google Scholar
5 Kwon YS, Nabeshima T, Shin EJ, Chun W, Jhoo JH, Jhoo WK et al . PAP 9704, a Korean herbal medicine attenuates methamphetamine-induced hyperlocomotion via adenosine A₂ receptor stimulation in mice. Biol Pharm Bull 2004; 27: 906–9.
10.1248/bpb.27.906
CAS PubMed Web of Science® Google Scholar
6 Kako M, Miura T, Nishiyama Y, Ichimaru M, Moriyasu M, Kato A. Hypoglycemic effect of the rhizomes of Polygala senega in normal and diabetic mice and its main component, the triterpenoid glycoside senegin-II. Planta Med 1996; 62: 440–3.
10.1055/s-2006-957935
CAS PubMed Web of Science® Google Scholar
7 Farina M, Franco JL, Ribas CM, Meotti FC, Missau FC, Pizzolatti MG et al . Protective effects of Polygala paniculata extract against methylmercury-induced neurotoxicity in mice. J Pharm Pharmacol 2005; 57: 1503–8.
10.1211/jpp.57.11.0017
CAS PubMed Web of Science® Google Scholar
8 Campos RO, Santos ARS, Vaz ZR, Pinheiro TR, Pizzolatti MG, Cechinel Filho V et al . Antinociceptive properties of the hydroalcoholic extract and preliminary study of a xanthone isolated from Polygala cyparissias (Polygalaceae). Life Sci 1997; 61: 1619–30.
10.1016/S0024-3205(97)00741-8
PubMed Web of Science® Google Scholar
9 Meotti FC, Ardenghi JV, Pretto JB, Souza WM, Moura JD, Cunha A et al . Antinociceptive properties of coumarins, steroid and dihydrostyryl-2-pyrones from Polygala sabulosa (Polygalaceae) in mice. J Pharm Pharmacol 2006; 58: 107–12.
10.1211/jpp.58.1.0013
CAS PubMed Web of Science® Google Scholar
10 Duarte FS, Duzzioni M, Mendes BG, Pizzolatti MG, Lima TCM. Participation of dihydrostyryl-2-pyrones and styryl-2-pyrones in the central effects of Polygala sabulosa (Polygalaceae), a folk medicine topical anesthetic. Pharmacol Biochem Behav 2007; 86: 150–61.
10.1016/j.pbb.2006.12.019
CAS PubMed Web of Science® Google Scholar
11 Cristiano R, Pizzolatti MG, Delle Monache F, Rezende CM, Bronco A. Two xanthones from Polygala paniculata and confirmation of the 1-hydroxy-2,3,5-trimethoxy-xanthone at trace level by HRGC-MS. Z Naturforsch [C] 2003; 58: 490–4.
10.1515/znc-2003-7-808
CAS PubMed Web of Science® Google Scholar
12 Pinheiro TR, Checinel-Filho V, Santos ARS, Calixto JB, Delle Monache F, Pizzolatti MG et al . Three xanthones from Polygala cyparissias. Phytochemistry 1998; 48: 725–8.
10.1016/S0031-9422(98)00051-X
CAS Web of Science® Google Scholar
13 Zhang D, Miyase T, Kuroyanagi M, Umehara K, Noguchi H. Polygalasaponins XLII-XLVI from roots of Polygala glomerata. Phytochemistry 1998; 47: 459–66.
10.1016/S0031-9422(97)00490-1
CAS PubMed Google Scholar
14 Ribeiro RA, Vale ML, Thomazzi SM, Paschoalato ABP, Poole S, Ferreira SH et al . Involvement of resident macrophages and mast cells in the writhing nociceptive response induced by zymosan and acetic acid in mice. Eur J Pharmacol 2000; 387: 111–8.
10.1016/S0014-2999(99)00790-6
CAS PubMed Web of Science® Google Scholar
15 Ikeda Y, Ueno A, Naraba H, Oh-ishi S. Involvement of vanilloid receptor VR1 and prostanoids in the acid-induced writhing responses of mice. Life Sci 2001; 69: 2911–9.
10.1016/S0024-3205(01)01374-1
CAS PubMed Web of Science® Google Scholar
16 Feng Y, Cui M, Willis W. Gabapentin markedly reduces acetic acid-induced visceral nociception. Anesthesiology 2003; 98: 729–33.
10.1097/00000542-200303000-00023
CAS PubMed Web of Science® Google Scholar
17 Zimmermann M. Ethical Guidelines for investigations of experimental pain in conscious animals. Pain 1983; 16: 109–10.
10.1016/0304-3959(83)90201-4
CAS PubMed Web of Science® Google Scholar
18 Pizzolatti MG, Luciano C, Delle Monache F. Styryl- and dihydrostyryl-2-pyrones derivatives from Polygala sabulosa. Phytochemistry 2000; 55: 819–22.
10.1016/S0031-9422(00)00301-0
CAS PubMed Web of Science® Google Scholar
19 Pizzolatti MG, Cunha Jr A, Pereira WS, Delle Monache F. A new styryl-2-pyrone derivative from Polygala sabulosa. Biochem Syst Ecol 2004; 32: 603–6.
10.1016/j.bse.2003.08.011
CAS Web of Science® Google Scholar
20 Beirith A, Santos ARS, Calixto JB. Mechanisms underlying the nociception and paw oedema caused by injection of glutamate into the mouse paw. Brain Res 2002; 924: 219–28.
10.1016/S0006-8993(01)03240-1
CAS PubMed Web of Science® Google Scholar
21 Scheidt C, Santos ARS, Ferreira J, Malheiros A, Cechinel-Filho V, Yunes RA et al. Evidence for the involvement of glutamatergic receptors in the antinociception caused in mice by the sesquiterpene drimanial. Neuropharmacology 2002; 43: 340–7.
10.1016/S0028-3908(02)00117-X
CAS PubMed Web of Science® Google Scholar
22 Hylden JL, Wilcox GL. Intrathecal morphine in mice: a new technique. Eur J Pharmacol 1980; 67: 313–6.
10.1016/0014-2999(80)90515-4
CAS PubMed Web of Science® Google Scholar
23 Choi SS, Han KJ, Lee JK, Lee HK, Han EJ, Kim DH et al . Antinociceptive mechanisms of orally administered decursinol in the mouse. Life Sci 2003; 73: 471–85.
10.1016/S0024-3205(03)00311-4
CAS PubMed Web of Science® Google Scholar
24 Sakurada T, Sugiyama A, Sakurada C, Tanno K, Sakurada S, Kisara K et al . Involvement of nitric oxide in spinally mediated capsaicin- and glutamate-induced behavioural responses in the mouse. Neurochem Int 1996; 29: 271–8.
10.1016/0197-0186(96)00004-6
CAS PubMed Web of Science® Google Scholar
25 Rodrigues ALS, Silva GL, Mateussi AS, Fernandes ES, Miguel OG, Yunes RA et al . Involvement of monoaminergic system in the antidepressant-like effect of the hydroalcoholic extract of Siphocampylus verticillatus. Life Sci 2002; 70: 1347–58.
10.1016/S0024-3205(01)01498-9
CAS PubMed Web of Science® Google Scholar
26 Fundytus ME. Glutamate receptors and nociception: implications for the drug treatment of pain. CNS Drugs 2001; 15: 29–58.
10.2165/00023210-200115010-00004
CAS PubMed Web of Science® Google Scholar
27 Millan MJ. The induction of pain: an integrative review. Prog Neurobiol 1999; 57: 1–164.
10.1016/S0301-0082(98)00048-3
CAS PubMed Web of Science® Google Scholar
28 Neugebauer V. Metabotropic glutamate receptors-important modulators of nociception and pain behavior. Pain 2002; 98: 1–8.
10.1016/S0304-3959(02)00140-9
CAS PubMed Web of Science® Google Scholar
29 Lipton SA, Rosenberg PA. Mechanisms of disease. Excitatory amino acids as a final common pathway for neurological disorders. N Eng J Med 1994; 330: 613–22.
Google Scholar
30 Bernardino L, Xapelli S, Silva AP, Jakobsen B, Poulsen FR, Oliveira CR et al . Modulator effects of interleukin-1β and tumor necrosis factor-α on AMPA-induced excitotoxicity in mouse organotypic hippocampal slice cultures. J Neurosci 2005; 25: 6734–44.
10.1523/JNEUROSCI.1510-05.2005
CAS PubMed Web of Science® Google Scholar
31 Caruso C, Durand D, Watanobe H, Lasaga M. NMDA and group I metabotropic glutamate receptors activation modulates substance P release from the arcuate nucleus and median eminence. Neurosci Lett 2006; 393: 60–4.
10.1016/j.neulet.2005.09.042
CAS PubMed Web of Science® Google Scholar
32 Liu H, Mantyh PW, Basbaum AI. NMDA-receptor regulation of substance P release from primary afferent nociceptors. Nature 1997; 386: 721–4.
10.1038/386721a0
CAS PubMed Web of Science® Google Scholar
33 Pan W, Zadina JE, Harlan RE, Weber JT, Banks WA, Kastin AJ. Tumor necrosis factor-α: a neuromodulator in the CNS. Neurosci Biobehav Rev 1997; 21: 603–13.
10.1016/S0149-7634(96)00047-4
CAS PubMed Web of Science® Google Scholar
34 Mata R, Rojas A, Acevedo L, Estrada S, Calzada F, Rojas I et al . Smooth muscle relaxing flavonoids and terpenoids from Conyza filaginoides. Planta Med 1997; 63: 31–5.
10.1055/s-2006-957598
CAS PubMed Web of Science® Google Scholar

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Volume103, Issue1

July 2008

Pages 43-47

Antinociceptive Effect of the Polygala sabulosa Hydroalcoholic Extract in Mice: Evidence for the Involvement of Glutamatergic Receptors and Cytokine Pathways

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Antinociceptive Effect of the Polygala sabulosa Hydroalcoholic Extract in Mice: Evidence for the Involvement of Glutamatergic Receptors and Cytokine Pathways

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