Effect of platelet-released growth factors and collagen type I on osseous regeneration of mandibular defects
A pilot study in minipigs
Gabor Fuerst
Department of Oral Surgery, University of Vienna School of Dentistry, Vienna, Austria
Ludwig Boltzmann Institute for Oral Implantology, Vienna, Austria
Search for more papers by this authorGruber Reinhard
Department of Oral Surgery, University of Vienna School of Dentistry, Vienna, Austria
Ludwig Boltzmann Institute for Oral Implantology, Vienna, Austria
Search for more papers by this authorStefan Tangl
Department of Oral Surgery, University of Vienna School of Dentistry, Vienna, Austria
Search for more papers by this authorMartina Mittlböck
Section of Clinical Biometrics, Department of Medical Computer Sciences, Vienna Medical School, University of Vienna, Vienna, Austria
Search for more papers by this authorFidel Sanroman
Department of Animal Pathology at the Veterinary Faculty, University Complutense, Madrid, Spain
Search for more papers by this authorGeorg Watzek
Department of Oral Surgery, University of Vienna School of Dentistry, Vienna, Austria
Ludwig Boltzmann Institute for Oral Implantology, Vienna, Austria
Search for more papers by this authorGabor Fuerst
Department of Oral Surgery, University of Vienna School of Dentistry, Vienna, Austria
Ludwig Boltzmann Institute for Oral Implantology, Vienna, Austria
Search for more papers by this authorGruber Reinhard
Department of Oral Surgery, University of Vienna School of Dentistry, Vienna, Austria
Ludwig Boltzmann Institute for Oral Implantology, Vienna, Austria
Search for more papers by this authorStefan Tangl
Department of Oral Surgery, University of Vienna School of Dentistry, Vienna, Austria
Search for more papers by this authorMartina Mittlböck
Section of Clinical Biometrics, Department of Medical Computer Sciences, Vienna Medical School, University of Vienna, Vienna, Austria
Search for more papers by this authorFidel Sanroman
Department of Animal Pathology at the Veterinary Faculty, University Complutense, Madrid, Spain
Search for more papers by this authorGeorg Watzek
Department of Oral Surgery, University of Vienna School of Dentistry, Vienna, Austria
Ludwig Boltzmann Institute for Oral Implantology, Vienna, Austria
Search for more papers by this authorAbstract
Objective: To study the effects of platelet-released growth factors (PRGF) and collagen type I on bone defect healing in minipig mandibles.
Material and Methods: In eight adult minipigs defects were trephined in the facial mandibular wall from extra-oral and filled with collagen+PRGF or with collagen alone. Control defects were left untreated. PRGF were defined as the supernatants obtained after centrifugation of washed, thrombin-activated allogenic cells of platelet-rich plasma. The animals were sacrificed at 4 and 8 weeks. For histological analysis, undecalcified ground specimens stained with the Levai–Laczko stain were used.
Results: For the entire follow-up, the amount of newly formed bone was 35.49±3.84% in the collagen+PRGF group, 46.34±3.84% in the collagen-only group and 33.83±4.11% in the controls. The differences between the collagen+PRGF and the collagen-only group (p=0.0343), and between the collagen-only group and the controls (p=0.0305) were significant. Histologically, defects filled with collagen+PRGF showed inflammatory reactions at 4 weeks, and new bone formation near the remnants of the filler collagen was reduced.
Conclusion: The data suggest that collagen type I alone, but not its combination with PRGF can support the early stages of cortical bone repair.
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