Activation of human platelet-rich plasmas: effect on growth factors release, cell division and in vivo bone formation
Yanik Roussy, Marie-Pierre Bertrand Duchesne,
Guy Gagnon,
Marie-Pierre Bertrand Duchesne
Faculté de Médecine dentaire, Quebec, QC, Canada
Search for more papers by this authorYanik Roussy, Marie-Pierre Bertrand Duchesne,
Guy Gagnon,
Marie-Pierre Bertrand Duchesne
Faculté de Médecine dentaire, Quebec, QC, Canada
Search for more papers by this authorCorrespondence to:
Dr Gagnon Guy
Faculte de Medecine dentaireUniversite Laval, Quebec, QCCanada G1K 7P4Tel.: (418) 656 2131 ext 8126Fax: (418) 656 2720e-mail: [email protected]
Abstract
Objectives: Aims of this controlled study were to determine the effects of activated human platelet-rich plasmas (PRPs) on early and mature bone formation in vivo, and to characterize the effect of PRP activation on growth factors release and endothelial cell division in vitro.
Material and methods: PRPs were prepared from four volunteers with the platelet concentrate collector system (PCCS) system and activated with three concentrations of calcium and thrombin. Platelet-derived growth factor (PDGF)-BB, vascular endothelial growth factor (VEGF), transforming growth factor β (TGF-β) and interleukin-1β (IL-1β) levels released in supernatants were measured by ELISA, at time 0, 1h, 24h and 6 days following PRP activation. Mitogenic potential of PRP supernatants were tested on endothelial cells in vitro, and the effects of activated human PRPs on bone formation in vivo were measured in athymic rats by micro-CT analyses.
Results: Activation of PRPs with calcium and thrombin triggered an immediate release of VEGF, PDGF-BB and TGF-β and a delayed release of IL-1β in PRP supernatants. Higher endothelial cell division was observed with supernatants from activated PRPs than from non-activated PRPs. Positive correlations were observed between VEGF levels and endothelial cell division and bone formation. A negative correlation was also found between PDGF-BB concentration and bone formation. However, early and mature bone formations with activated PRPs did not significantly differ from the ones obtained in the control group.
Conclusions: Activation of PRPs with calcium and thrombin regulates growth factors release and endothelial cell division in vitro. However, activated PRPs does not improve the early or mature bone formations in vivo in this athymic rat model.
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