Biocompatibility of different barrier membranes in cultures of human CRL 11372 osteoblast-like cells: an immunohistochemical study
Ayhan Bilir
Department of Histology and Embriology, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey
Search for more papers by this authorBuket Aybar
Department of Oral Surgery, School of Dentistry, Istanbul University, Istanbul, Turkey
Search for more papers by this authorSinasi H. Tanrikulu
Department of Oral Surgery, School of Dentistry, Istanbul University, Istanbul, Turkey
Search for more papers by this authorHalim Issever
Department of Public Health, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey
Search for more papers by this authorSevilcan Tuna
Department of Histology and Embriology, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey
Search for more papers by this authorAyhan Bilir
Department of Histology and Embriology, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey
Search for more papers by this authorBuket Aybar
Department of Oral Surgery, School of Dentistry, Istanbul University, Istanbul, Turkey
Search for more papers by this authorSinasi H. Tanrikulu
Department of Oral Surgery, School of Dentistry, Istanbul University, Istanbul, Turkey
Search for more papers by this authorHalim Issever
Department of Public Health, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey
Search for more papers by this authorSevilcan Tuna
Department of Histology and Embriology, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey
Search for more papers by this authorAbstract
Objective: The aim of this study was to evaluate the biocompatibility of two different barrier materials in cultures of human osteoblast-like cells (CRL 11372) in vitro.
Material and methods: Polylactic acid (Epi-Guide®; EG) and collagen membrane (Bio-Collagen®, BC) were examined. To analyze the effect of materials on cell proliferation, cell numbers and cell viability, cells were cultured on the barrier membranes for 24 and 72 h. Cells plated on culture dishes (CD) served as positive controls. Cell proliferation rate was assessed by the bromodeoxyuridine (BrdU) immunohistochemical technique. The cell numbers of each well were counted. Cell viability was estimated by counting the number of cells, which excluded trypan blue solution. Scanning electron microscopy (SEM) was used to observe the interactions between osteoblastic cells and barrier membranes.
Results: The highest number of BrdU-labeled cells were seen on CD after both of the time periods. In comparison with the other two groups, BC showed significantly fewer cells after both time periods. Regarding cell numbers, after 24 and 72 h of incubations CD showed the highest number of cells. The number of viable cells was similar for all the groups. After 72 h for the EG group, SEM view showed flat cells. After 72-h time periods, the BC group revealed a weak adhesion of cells to the barriers.
Conclusion: The results demonstrate that cells were able to proliferate on these materials and EG promoted the proliferation of human osteoblast like cells. We prefer to use the EG membrane.
References
- Alpar, B., Leyhausen, G., Gunay, H. & Geurtsen, W. (2000) Compatibility of resorbable and nonresorbable guided tissue regeneration membranes in cultures of primary human periodontal ligament fibroblasts and human osteoblast-like cells. Clinical Oral Investigations 4: 219–225.
-
Amenta, J.S.,
Mehta, J. &
Baccino, F.M. (1988) Protocolysis associated with thymidine-induced selective cell death in L-cell cultures. In: F. Feo,
P. Pami &
A. Columbano, eds.
Chemical Carcinogenesis, Model and Mechanisms, 535–551. New York: Plenum Press.
10.1007/978-1-4757-9640-7_59 Google Scholar
- Amenta, J.S., Sargus, M.J., Baccino, F.M., Sacchi, C. & Bonelli, G. (1993) Cell death induced in L-cells by treatment with thymidine: staging of the process and relationship to apoptosis. In Vitro Cellular and Developmental Biology – Animal 29A: 855–861.
- Archer, C.W., Rooney, P. & Wolpert, L. (1982) Cell shape and cartilage differentiation of early chick limb bud cells in culture. Cell Differention 11: 245–251.
- Aybar, B., Bilir, A., Akcakaya, H. & Ceyhan, T. (2004a) Effects of tricalcium phosphate bone graft materials on primary cultures of osteoblast cells in vitro. Clinical Oral Implants Research 15: 119–125.
- Aybar, B., Erkut, S., Yildirim, S. & Bilir, A. (2004b) The effects of naproxen sodium on specific parameters of L-strain cells over guided bone regeneration barrier materials. Clinical Oral Implants Research 15: 709–715.
- Bilir, A., Altinoz, M.A., Attar, E., Erkan, M. & Aydiner, A. (2002) Acetaminophen modulations of chemotherapy efficacy in MDAH 2774 human endometrioid ovarian cancer cells in vitro. Neoplasma 49: 38–42.
- Bowers, K.T., Keller, J.C., Randolph, B.A., Wick, D.G. & Michaels, C.M. (1992) Optimization of surface micromorphology for enhanced osteoblast responses in vitro. The International Journal of Oral & Maxillofacial Implants 7: 302–310.
- Bragger, U., Hammerle, C.H. & Lang, N.P. (1996) Immediate transmucosal implants using the principle of guided tissue regeneration (II). A cross-sectional study comparing the clinical outcome 1 year after immediate to standard implant placement. Clinical Oral Implants Research 7: 268–276.
- Brunette, D.M. (1988) The effects of implant surface topography on the behavior of cells. The International Journal of Oral & Maxillofacial Implants 3: 231–246.
- Buser, D., Bragger, U., Lang, N.P. & Nyman, S. (1990) Regeneration and enlargement of jaw bone using guided tissue regeneration. Clinical Oral Implants Research 1: 22–32.
- Caffesse, R.G., Smith, B.A., Duff, B., Morrison, E.C., Merril, D. & Becker, W. (1990) Class II furcations treated by guided tissue regeneration in humans: case reports. Journal of Periodontology 61: 510–514.
- Dahlin, C., Linde, A., Gottlow, J. & Nyman, S. (1988) Healing of bone defects by guided tissue regeneration. Plastic and Reconstructive Surgery 81: 672–676.
- Eickholz, P., Kim, T.S., Steinbrenner, H., Dorfer, C. & Holle, R. (2000) Guided tissue regeneration with bioabsorbable barriers: intrabony defects and class II furcations. Journal of Periodontology 71: 999–1008.
- Emonard, H., Grimaud, J.A., Nusgens, B., Lapiere, C.M. & Foidart, J.M. (1987) Reconstituted basement-membrane matrix modulates fibroblast activities in vitro. Journal of Cellular Physiology 133: 95–102.
- Folkman, J. & Moscona, A. (1978) Role of cell shape in growth control. Nature 273: 345–349.
- Glantz, A.S. (1997) Primer of Biostatistics (Chapter 6). 4th edition, 171–175. USA: McGraw-Hill Co.
- Gottlow, J. (1993) Guided tissue regeneration using bioresorbable and non-resorbable devices: initial healing and long-term results. Journal of Periodontology 64 (1 Suppl.): 1157–1165.
- Gottlow, J., Nyman, S., Lindhe, J., Karring, T. & Wennstrom, J. (1986) New attachment formation in the human periodontium by guided tissue regeneration. Case reports. Journal of Clinical Periodontology 13: 604–616.
- Hammerle, C.H. (1999) Membranes and bone substitutes in guided bone regeneration. Proceedings of the 3rd European Workshop on Periodontology 3: 468–499.
- Hammerle, C.H. & Lang, N.P. (2001) Single stage surgery combining transmucosal implant placement with guided bone regeneration and bioresorbable materials. Clinical Oral Implants Research 12: 9–18.
- Hammerle, C.H., Olah, A.J., Schmid, J., Fluckiger, L., Gogolewski, S., Winkler, J.R. & Lang, N.P. (1997) The biological effect of natural bone mineral on bone neoformation on the rabbit skull. Clinical Oral Implants Research 8: 198–207.
- Karring, T., Nyman, S., Gottlow, J. & Laurell, L. (1993) Development of the biological concept of guided tissue regeneration – animal and human studies. Periodontology 2000 1: 26–35.
- Kononen, M., Hormia, M., Kivilahti, J., Hautaniemi, J. & Thesleff, I. (1992) Effect of surface processing on the attachment, orientation, and proliferation of human gingival fibroblasts on titanium. Journal of Biomedical Materials Research 26: 1325–1341.
- Lang, N.P., Hammerle, C.H., Bragger, U., Lehmann, B. & Nyman, S.R. (1994) Guided tissue regeneration in jawbone defects prior to implant placement. Clinical Oral Implants Research 5: 92–97.
- Locci, P., Calvitti, M., Belcastro, S., Pugliese, M., Guerra, M., Marinucci, L., Staffolani, N. & Becchetti, E. (1997) Phenotype expression of gingival fibroblasts cultured on membranes used in guided tissue regeneration. Journal of Periodontology 68: 857–863.
- Marinucci, L., Lilli, C., Baroni, T., Becchetti, E., Belcastro, S., Balducci, C. & Locci, P. (2001) In vitro comparison of bioabsorbable and non-resorbable membranes in bone regeneration. Journal of Periodontology 72: 753–759.
- McGinnis, M., Larsen, P., Miloro, M. & Beck, F.M. (1998) Comparison of resorbable and nonresorbable guided bone regeneration materials: a preliminary study. The International Journal of Oral & Maxillofacial Implants 13: 30–35.
- Nyman, S., Lang, N.P., Buser, D. & Bragger, U. (1990) Bone regeneration adjacent to titanium dental implants using guided tissue regeneration: a report of two cases. The International Journal of Oral & Maxillofacial Implants 5: 9–14.
- Rothamel, D., Schwarz, F., Sculean, A., Herten, M., Scherbaum, W. & Becker, J. (2004) Biocompatibility of various collagen membranes in cultures of human PDL fibroblasts and human osteoblast-like cells. Clinical Oral Implants Research 15: 443–449.
- Royce, P.M. & Barnes, M.J. (1988) Interaction of embryonic chick calvarial bone cells with collagen substrata; attachment characteristics and growth behaviour. Connective Tissue Research 17: 55–70.
- Santini, M.T., Rainaldi, G. & Indovina, P.L. (2000) Apoptosis, cell adhesion and the extracellular matrix in the three-dimensional growth of multicellular tumor spheroids. Critical Reviews in Oncology/Hematology 36: 75–87.
- Selvig, K.A., Kersten, B.G., Chamberlain, A.D., Wikesjo, U.M. & Nilveus, R.E. (1992) Regenerative surgery of intrabony periodontal defects using ePTFE barrier membranes: scanning electron microscopic evaluation of retrieved membranes versus clinical healing. Journal of Periodontology 63: 974–978.
- Sigurdsson, T.J., Tatakis, D.N., Lee, M.B. & Wikesjo, U.M. (1995) Periodontal regenerative potential of space-providing expanded polytetrafluoroethylene membranes and recombinant human bone morphogenetic proteins. Journal of Periodontology 66: 511–521.
- Takata, T., Wang, H.L. & Miyauchi, M. (2001) Attachment, proliferation and differentiation of periodontal ligament cells on various guided tissue regeneration membranes. Journal of Periodontal Research 36: 322–327.
- Teparat, T., Solt, C.W., Claman, L.J. & Beck, F.M. (1998) Clinical comparison of bioabsorbable barriers with non-resorbable barriers in guided tissue regeneration in the treatment of human intrabony defects. Journal of Periodontology 69: 632–641.
- Vouros, I., Aristodimou, E. & Konstantinidis, A. (2004) Guided tissue regeneration in intrabony periodontal defects following treatment with two bioabsorbable membranes in combination with bovine bone mineral graft. A clinical and radiographic study. Journal of Clinical Periodontology 31: 908–917.
- Wang, H.L., Miyauchi, M. & Takata, T. (2002) Initial attachment of osteoblasts to various guided bone regeneration membranes: an in vitro study. Journal of Periodontal Research 37: 340–344.
Citing Literature
