Use of platelet-rich plasma under autogenous onlay bone grafts
Sandra Regina Miranda
Department of Oral and Maxillofacial Surgery, Universidade do Sagrado Coração – USC, Bauru, São Paulo, Brazil
Search for more papers by this authorHugo Nary Filho
Department of Oral and Maxillofacial Surgery, Universidade do Sagrado Coração – USC, Bauru, São Paulo, Brazil
Search for more papers by this authorLuís Eduardo Marques Padovan
Department of Oral and Maxillofacial Surgery, Universidade do Sagrado Coração – USC, Bauru, São Paulo, Brazil
Search for more papers by this authorDaniel Araki Ribeiro
Center for Genotoxins and Carcinogens Evaluation – TOXICAN, Department of Pathology, Faculdade de Medicina de Botucatu, UNESP, Botucatu, São Paulo, Brazil
Search for more papers by this authorDaniela Nicolielo
Biochemistry pharmaceutical, Hematology Section of the Clinical Analysis Laboratory, Universidade do Sagrado Coração – USC, Bauru, São Paulo, Brazil
Search for more papers by this authorMariza Akemi Matsumoto
Department of Oral and Maxillofacial Surgery, Universidade do Sagrado Coração – USC, Bauru, São Paulo, Brazil
Search for more papers by this authorSandra Regina Miranda
Department of Oral and Maxillofacial Surgery, Universidade do Sagrado Coração – USC, Bauru, São Paulo, Brazil
Search for more papers by this authorHugo Nary Filho
Department of Oral and Maxillofacial Surgery, Universidade do Sagrado Coração – USC, Bauru, São Paulo, Brazil
Search for more papers by this authorLuís Eduardo Marques Padovan
Department of Oral and Maxillofacial Surgery, Universidade do Sagrado Coração – USC, Bauru, São Paulo, Brazil
Search for more papers by this authorDaniel Araki Ribeiro
Center for Genotoxins and Carcinogens Evaluation – TOXICAN, Department of Pathology, Faculdade de Medicina de Botucatu, UNESP, Botucatu, São Paulo, Brazil
Search for more papers by this authorDaniela Nicolielo
Biochemistry pharmaceutical, Hematology Section of the Clinical Analysis Laboratory, Universidade do Sagrado Coração – USC, Bauru, São Paulo, Brazil
Search for more papers by this authorMariza Akemi Matsumoto
Department of Oral and Maxillofacial Surgery, Universidade do Sagrado Coração – USC, Bauru, São Paulo, Brazil
Search for more papers by this authorAbstract
Objectives: To analyze the healing of autogenous onlay bone grafts in three different situations, focusing on the interface area.
Material and methods: Sixteen rabbits underwent autogenous bone graft surgeries in the calvaria. The block bone grafts were positioned in three different situations: direct contact between bone graft and receptor bed, graft interposed by particulate bone, and graft interposed by platelet-rich plasma (PRP). After 7, 15, 30, and 60 days, the specimens were retrieved for histological and morphometric evaluation.
Results: All groups healed uneventfully and presented incorporation of the grafts after 30 days. A slightly more evident new bone formation could be observed in the PRP group in the first analyzed period, and an earlier maturation of bone in the last period, although no statistically significant differences were achieved.
Conclusion: The use of additional material between the bone graft and the receptor bed when using the onlay technique must be carefully considered, taking into account the size of the reconstruction and the cost/benefit relation. The addition of PRP in between autogenous bone blocks and the receptor bed did not confer significant benefit for the new bone formation and healing on the calvaria of bone of rabbits.
References
- Aghaloo, T.L., Moy, P.K. & Freymiller, E.G. (2002) Investigation of platelet-rich plasma in rabbit cranial defects: a pilot study. Journal of Oral and Maxillofacial Surgery 60: 1176–1181.
- Alberius, P., Gordh, M., Lindberg, L. & Johnell, O. (1996) Effect of cortical perforation of both graft and host bed on onlay incorporation to the rat skull. European Journal of Oral Science 104: 5–6.
- Al-Ruhaimi, K.A. (2001) Bone grafts substitutes: a comparative qualitative histologic review of current osteoconductive grafting materials. International Journal of Oral & Maxillofacial Implants 16: 105–114.
- Arrington, E.D., Smith, W.J., Chambers, H.G., Bucknell, A.L. & Davino, N.A. (1996) Complications of iliac crest bone graft harvesting. Clinical Orthopaedics Related Research 329: 300–309.
- Breine, U. & Branemark, P.-I. (1980) Reconstruction of alveolar jaw bone. Scandinavian Journal of Plastic and Reconstructive Surgery 14: 14–48.
- Carvalho, P.S.P., Vasconcellos, L.W. & Pi, J. (2000) Influence of bed preparation on the incorporation of autogenous bone grafts: a study in dogs. International Journal of Oral & Maxillofacial Implants 15: 565–570.
- Fürst, G., Gruber, R., Tangl, S., Zechner, W., Haas, R., Mailath, G., Sanroman, F. & Watzek, G. (2003) Sinus grafting with autogenous platelet-rich plasma and bovine hydroxyapatite. A histomorphometric study in minipigs. Clinical Oral Implants Research 14: 500–508.
- Froum, S.J., Wallace, S.S., Tarnow, D.P. & Cho, S.C. (2002) Effect of platelet-rich plasma on bone growth and osseointegration in human maxillary sinus grafts: three bilateral case reports. International Journal of Periodontics and Restorative Dentistry 22: 45–53.
- Garg, A.K., Morales, M.J., Navarro, I. & Duarte, F. (1998) Autogenous mandibular bone grafts in the treatment of the resorbed maxillary anterior alveolar ridge: rationale and approach. Implant Dentistry 7: 169–176.
- Goldstein, J., Mase, C. & Newman, M.H. (1993) Fixed membranous bone graft survival after recipient bed alteration. Plastic and Reconstructive Surgery 91: 589–596.
- Gonshor, A. (2002) Technique for producing platelet-rich plasma and platelet concentrate: background and process. International Journal of Periodontics and Restorative Dentistry 22: 547–557.
- Gordh, M., Alberius, P., Lindberg, L. & Johnell, O. (1997) Bone graft incorporation after cortical perforations of the host bed. Otolaryngology Head and Neck Surgery 117: 664–670.
- Hoppenreijs, T.J.M., Nijdam, E.S. & Freihofer, H.P.M. (1992) The chin as a donor site in early secondary osteoplasty: a retrospective clinical and radiological evaluation. Journal of Craniomaxillofacial Surgery 20: 119–124.
- Jakse, N., Tangl, S., Gilli, R., Berghold, A., Lorenzoni, M., Eskici, A., Haas, R. & Pertl, C. (2003) Influence of PRP on autogenous sinus grafts. An experimental study on sheep. Clinical Oral Implants Research 14: 578–583.
- Keller, E., Tolman, D. & Eckert, S. (1999) Surgical-prosthodontic reconstruction of advanced maxillary bone compromise with autogenous onlay block bone grafts and osseointegrated endosseous implants: a 12-year study of 32 consecutive patients. International Journal of Oral & Maxillofacial Implants 14: 197–209.
- Landesberg, R., Roy, M. & Glickman, R.S. (2000) Quantification of growth factor levels using a simplified method of platelet-rich plasma gem preparation. Journal of Oral and Maxillofacial Surgery 58: 297–300.
- Marx, R.E. (2001) Platelet-rich plasma (PRP): what is PRP and what is not PRP. Implant Dentistry 10: 225–228.
- Marx, R.E. (2004) Platelet-rich plasma: evidence to support its use. Journal of Oral and Maxillofacial Surgery 62: 489–496.
- Marx, R.E., Carlson, E.R., Eichstaedt, R.M., Schimmele, S.R., Strauss, J.E. & Georgeff, K.R. (1998) Platelet-rich plasma. Growth factor enhancement for bone grafts. Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontics 85: 638–646.
- Mazor, Z., Peleg, M., Garg, A.K. & Luboshitz, J. (2004) Platelet–rich plasma for bone graft enhancement in sinus floor augmentation with simultaneous implant placement: patient series study. Implant Dentistry 13: 65–72.
- Misch, C.M. (1997) Comparison of intraoral donor sites for onlay grafting prior to implant placement. International Journal of Oral & Maxillofacial Implants 12: 767–776.
- Petrugaro, P.S. (2001) The use of platelet rich plasma with growth factors (autologous platelet gel) to enhance hard and soft tissue healing and maturation in the reconstruction of the maxillary pneumatized sinus. Contemporary Periodontics and Implantology 1: 1–7.
- Phillips, J.H. & Rahn, B.A. (1990) Fixation effects on membranous and endochondral onlay bone graft revascularization and bone deposition. Plastic and Reconstructive Surgery 85: 891–897.
- Rosenberg, E.S., Dent, H.D. & Torosian, J. (2000) Sinus grafting using platelet-rich plasma: initial case presentation. Practical Periodontics and Aesthetic Dentistry 12: 843–850.
- Shanaman, R., Filstein, M.R. & Danesh-Meyer, M.J. (2001) Localized ridge augmentation using GBR and platelet-rich plasma: case reports. International Journal of Periodontics and Restorative Dentistry 21: 345–355.
- Van Steenberghe, D., Naert, I., Bossuyt, M., De Mars, G., Calberson, L., Ghyselen, J. & Branemark, P-I. (1997) The rehabilitation of the severely resorbed maxilla by simultaneous placement of autogenous bone grafts and implants: a 10-year evaluation. Clinical Oral Investigation 1: 102–108.
- Tayapongsak, P., O'Brien, D.A., Monteiro, C.B. & Arceo-Diaz, L.Y. (1994) Autologous fibrin adhesive in mandibular reconstruction with particulate cancellous bone and marrow. Journal of Oral and Maxillofacial Surgery 52: 161–165.
- Thor, A. (2002) Reconstruction of the anterior maxilla with platelet gel, autogenous bone, and titanium mesh: a case report. Clinical Implant Dentistry Related Research 4: 150–155.
- Tolman, D.E. (1995) Reconstructive procedures with endosseous implants in grafted bone: a review of the literature. International Journal of Oral & Maxillofacial Implants 10: 275–294.
- Velich, N., Nemeth, Z., Toth, C. & Szabo, G. (2004) Long–term results with different bone substitutes used for sinus floor elevation. Journal of Craniofacial Surgery 15: 38–41.
- Vercellotti, T. (2000) Piezoelectric surgery in implantology: a case report – A new piezoelectric ridge expansion technique. International Journal of Periodontics Restorative Dentistry 20: 359–365.
- Weilbrich, G., Hansen, T., Kleis, W., Buch, R. & Hitzler, W.E. (2004) Effect of platelet concentration in platelet-rich plasma on peri-implant bone regeneration. Bone 34: 665–671.
- Weilbrich, G. & Kleis, W.K.G. (2002) Curasan PRP kit vs. PCCS PRP system collection efficiency and platelet counts of two different methods for the preparation of platelet-rich plasma. Clinical Oral Implants Research 13: 437–443.
- Weilbrich, G., Kleis, W.K.G., Buch, R., Hitzler, W.E. & Hafner, G. (2003) The Harvest Smart PReP™ system versus the Friadent-Schütze platelet-rich plasma kit. Clinical Oral Implants Research 14: 233–239.
- Whitman, D.H., Berry, R.L. & Green, D.M. (1997) Platelet gel: an autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. Journal of Oral and Maxillofacial Surgery 55: 1294–1299.
- Wiltfang, J., Kloss, F.R., Kessler, P., Nkenke, E., Schultze-Mosgau, S., Zimmermann, R. & Schlegel, K.A. (2004) Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects. An animal experiment. Clinical Oral Implants Research 15: 187–193.
- Wiltfang, J., Schegel, K.A., Schultze-Mosgau, S., Nkenke, E., Zimmermann, R. & Kessler, P. (2003) Sinus floor augmentation with beta-tricalciumphosphate (beta-TCP): does platelet-rich plasma promote its osseous integration and degradation. Clinical Oral Implants Research 14: 213–218.
- Yazawa, M., Ogata, H., Kimura, A., Nakajima, T., Mori, T. & Watanabe, N. (2004) Basic studies on the bone formation ability by platelet rich plasma in rabbits. Journal of Craniofacial Surgery 15: 439–446.
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