Analysis of molecular determinants of PRL-3
Mihaela Pascaru
Department of Enzymology, Institute of Biochemistry, Bucharest, Romania
These authors contributed equally to this work.
Search for more papers by this authorCarmen Tanase
Department of Enzymology, Institute of Biochemistry, Bucharest, Romania
These authors contributed equally to this work.
Search for more papers by this authorAndrei M. Vacaru
Department of Enzymology, Institute of Biochemistry, Bucharest, Romania
Search for more papers by this authorPatricia Boeti
Center of General Surgery and Liver Transplantation, Fundeni Clinical Institute, Bucharest
Search for more papers by this authorElena Neagu
Center of Bioanalysis, National Institute for Research – Development of Biological Sciences, Bucharest, Romania
Search for more papers by this authorIrinel Popescu
Center of General Surgery and Liver Transplantation, Fundeni Clinical Institute, Bucharest
Search for more papers by this authorCorresponding Author
Stefan E. Szedlacsek
Department of Enzymology, Institute of Biochemistry, Bucharest, Romania
Correspondence to: Stefan E. SZEDLACSEK, Department of Enzymology / Institute of Biochemistry, Spl. Independentei 296, 060031 Bucharest, Romania.Tel.: + 40-21-2239069Fax: +40-21-2239068E-mail: [email protected]Search for more papers by this authorMihaela Pascaru
Department of Enzymology, Institute of Biochemistry, Bucharest, Romania
These authors contributed equally to this work.
Search for more papers by this authorCarmen Tanase
Department of Enzymology, Institute of Biochemistry, Bucharest, Romania
These authors contributed equally to this work.
Search for more papers by this authorAndrei M. Vacaru
Department of Enzymology, Institute of Biochemistry, Bucharest, Romania
Search for more papers by this authorPatricia Boeti
Center of General Surgery and Liver Transplantation, Fundeni Clinical Institute, Bucharest
Search for more papers by this authorElena Neagu
Center of Bioanalysis, National Institute for Research – Development of Biological Sciences, Bucharest, Romania
Search for more papers by this authorIrinel Popescu
Center of General Surgery and Liver Transplantation, Fundeni Clinical Institute, Bucharest
Search for more papers by this authorCorresponding Author
Stefan E. Szedlacsek
Department of Enzymology, Institute of Biochemistry, Bucharest, Romania
Correspondence to: Stefan E. SZEDLACSEK, Department of Enzymology / Institute of Biochemistry, Spl. Independentei 296, 060031 Bucharest, Romania.Tel.: + 40-21-2239069Fax: +40-21-2239068E-mail: [email protected]Search for more papers by this authorAbstract
In order to analyse whether a C-terminal polybasic sequence represents a nuclear localization signal (NLS) we obtained several truncated and mutant forms of protein of regerating liver (PRL)-3 and evaluated their subcellular localization as compared to the wild-type form. Our results invalidate the hypothesis that this is an NLS. We also analysed the influence of the C- and N-terminal residues on the phosphatase activity of PRL-3. Our results provide in vitro evidence that the C-terminal CAAX motif, besides directing the protein farnesylation, plays an additional regulatory role by inhibiting the catalytic efficiency of PRL-3. Taking into account the results we obtained, as well as reported data, we propose a hypothetical molecular mechanism for the nucleocytoplasmic localization and transfer of PRL-3.
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