Volume 254, Issue 1 pp. 87-94

LadR, a new PadR-related transcriptional regulator from Listeria monocytogenes, negatively regulates the expression of the multidrug efflux pump MdrL

Eugénie Huillet,

Eugénie Huillet

Equipe Contaminations Microbiennes, Unité de Recherche Pathologie Infectieuse et Immunologie, Inra, Nouzilly, France

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Philippe Velge,

Philippe Velge

Equipe Contaminations Microbiennes, Unité de Recherche Pathologie Infectieuse et Immunologie, Inra, Nouzilly, France

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Tatiana Vallaeys,

Tatiana Vallaeys

Dpt Microbiologie, Inra, INAPG, Thiverval Grignon, France

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Pierre Pardon,

Pierre Pardon

Equipe Contaminations Microbiennes, Unité de Recherche Pathologie Infectieuse et Immunologie, Inra, Nouzilly, France

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Eugénie Huillet,

Eugénie Huillet

Equipe Contaminations Microbiennes, Unité de Recherche Pathologie Infectieuse et Immunologie, Inra, Nouzilly, France

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Philippe Velge,

Philippe Velge

Equipe Contaminations Microbiennes, Unité de Recherche Pathologie Infectieuse et Immunologie, Inra, Nouzilly, France

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Tatiana Vallaeys,

Tatiana Vallaeys

Dpt Microbiologie, Inra, INAPG, Thiverval Grignon, France

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Pierre Pardon,

Pierre Pardon

Equipe Contaminations Microbiennes, Unité de Recherche Pathologie Infectieuse et Immunologie, Inra, Nouzilly, France

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First published: 14 November 2005

https://doi.org/10.1111/j.1574-6968.2005.00014.x

Citations: 8

Correspondence: Eugénie Huillet, Unité des Interactions Bactéries-Cellules, Institut Pasteur, Inserm U604, Inra USC2020, 28 rue du Dr Roux, 75724 Paris, France. Tel.: +33 1 45 68 37 79; fax: +33 1 45 68 87 06; e-mail: [email protected]

Editor: Jan-Ingmar Flock

Present address: Pierre Pardon, Unité Risqual, Ecole Nationale Vétérinaire d′Alfort, Maisons Alfort, France.

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Abstract

The Listeria monocytogenes genome encodes putative multidrug efflux transporters but only the MdrL transporter has been partially characterized in the wild-type LO28 strain. Here, we show in the LO28 strain, that the expression of MdrL is repressed at the transcriptional level, under standard growth conditions, by the product of a new gene ladR (lmo1408), and the expression of MdrL is induced in the presence of rhodamine. Phylogenetic analysis in related firmicutes shows that LadR, conserved in all sequenced Listeria genomes, forms an independent group from the large and diverse PadR transcriptional regulator family (PF03551). This is the first report of a bacterial multidrug transporter controlled by a member of the PadR family.

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Volume254, Issue1

January 2006

Pages 87-94

LadR, a new PadR-related transcriptional regulator from Listeria monocytogenes, negatively regulates the expression of the multidrug efflux pump MdrL

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LadR, a new PadR-related transcriptional regulator from Listeria monocytogenes, negatively regulates the expression of the multidrug efflux pump MdrL

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