Identification of the cadBA operon from Vibrio vulnificus and its influence on survival to acid stress

Jee Eun Rhee

Department of Food Science and Technology, Department of Molecular Biotechnology, Institute of Biotechnology, Chonnam National University, Kwang-Ju 500-757, South Korea

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Joon Haeng Rhee,

Joon Haeng Rhee

Department of Microbiology, Chonnam National University Medical School, Kwang-Ju 500-190, South Korea

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Phil Youl Ryu,

Phil Youl Ryu

Department of Microbiology, Chonnam National University Medical School, Kwang-Ju 500-190, South Korea

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Sang Ho Choi,

Corresponding Author

Sang Ho Choi

Department of Food Science and Technology, Department of Molecular Biotechnology, Institute of Biotechnology, Chonnam National University, Kwang-Ju 500-757, South Korea

*Corresponding author. Tel.: +82 (62) 530-2146; Fax: +82 (62) 530-2149, E-mail: [email protected]Search for more papers by this author

Jee Eun Rhee,

Jee Eun Rhee

Department of Food Science and Technology, Department of Molecular Biotechnology, Institute of Biotechnology, Chonnam National University, Kwang-Ju 500-757, South Korea

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Joon Haeng Rhee,

Joon Haeng Rhee

Department of Microbiology, Chonnam National University Medical School, Kwang-Ju 500-190, South Korea

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Phil Youl Ryu,

Phil Youl Ryu

Department of Microbiology, Chonnam National University Medical School, Kwang-Ju 500-190, South Korea

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Sang Ho Choi,

Corresponding Author

Sang Ho Choi

Department of Food Science and Technology, Department of Molecular Biotechnology, Institute of Biotechnology, Chonnam National University, Kwang-Ju 500-757, South Korea

*Corresponding author. Tel.: +82 (62) 530-2146; Fax: +82 (62) 530-2149, E-mail: [email protected]Search for more papers by this author

First published: 09 January 2006

https://doi.org/10.1111/j.1574-6968.2002.tb11089.x

Citations: 12

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Abstract

By a transposon-tagging method, cadBA genes encoding a lysine/cadaverin antiporter and a lysine decarboxylase were identified and cloned from Vibrio vulnificus. The deduced amino acid sequences of cadBA were 64–97% similar to those reported from other Enterobacteriaceae. Functions of cadBA genes on acid tolerance were assessed by comparing acid tolerances of V. vulnificus and its isogenic mutants, whose cadBA genes were separately inactivated by allelic exchanges. The results demonstrated that gene products of cadBA contribute to acid tolerance of V. vulnificus, and that their contribution is dependent on prior exposure of cells to moderately acidic pH.

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Volume208, Issue2

March 2002

Pages 245-251

Identification of the cadBA operon from Vibrio vulnificus and its influence on survival to acid stress

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Identification of the cadBA operon from Vibrio vulnificus and its influence on survival to acid stress

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