Identification of a promoter for the crystal protein-encoding gene cry1Ia from Bacillus thuringiensis subsp. kurstaki

Slim Tounsi

Centre de Biotechnologie de Sfax, Laboratoire de Protection et Transformation des Plantes, Unité Biopesticides et Métabolites, P.O. Box ‘K’, 3038 Sfax, Tunisia

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Samir Jaoua,

Corresponding Author

Samir Jaoua

Centre de Biotechnologie de Sfax, Laboratoire de Protection et Transformation des Plantes, Unité Biopesticides et Métabolites, P.O. Box ‘K’, 3038 Sfax, Tunisia

*Corresponding author. Tel.: +216 (74) 274110; Fax: +216 (74) 275970, E-mail: [email protected]Search for more papers by this author

Slim Tounsi,

Slim Tounsi

Centre de Biotechnologie de Sfax, Laboratoire de Protection et Transformation des Plantes, Unité Biopesticides et Métabolites, P.O. Box ‘K’, 3038 Sfax, Tunisia

Search for more papers by this author

Samir Jaoua,

Corresponding Author

Samir Jaoua

Centre de Biotechnologie de Sfax, Laboratoire de Protection et Transformation des Plantes, Unité Biopesticides et Métabolites, P.O. Box ‘K’, 3038 Sfax, Tunisia

*Corresponding author. Tel.: +216 (74) 274110; Fax: +216 (74) 275970, E-mail: [email protected]Search for more papers by this author

First published: 09 January 2006

https://doi.org/10.1111/j.1574-6968.2002.tb11084.x

Citations: 8

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Abstract

Expression of cry1Ia, one of the insecticidal protein genes of Bacillus thuringiensis subsp. kurstaki, was studied at the transcriptional level. By primer extension analysis, we have identified for the first time, the transcription start point of cry1Ia. Upstream from the cry1Ia transcription start point, was found a nucleotide sequence partially homologous to the consensus sequence for the E σ^E holoenzyme of Bacillus subtilis. Thus, it was strongly suggested that the identified cry1Ia promoter was under the control of σ³⁵, the B. thuringiensis homolog of σ^E. The transcriptional activity from the promoter was detected only for the sporulating cells at T2 and T5 stages.

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Volume208, Issue2

March 2002

Pages 215-218

Identification of a promoter for the crystal protein-encoding gene cry1Ia from Bacillus thuringiensis subsp. kurstaki

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Identification of a promoter for the crystal protein-encoding gene cry1Ia from Bacillus thuringiensis subsp. kurstaki

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