Journal of Cardiovascular Electrophysiology

Volume 20, Issue 9 pp. 1039-1045

Heterogeneous Expression of Potassium Currents and Pacemaker Currents Potentially Regulates Arrhythmogenesis of Pulmonary Vein Cardiomyocytes

YAO-CHANG CHEN M.Sc.,

YAO-CHANG CHEN M.Sc.

Department of Biomedical Engineering, National Defense Medical Center, Taipei, Taiwan

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NAN-HUNG PAN M.D.,

NAN-HUNG PAN M.D.

Division of Cardiovascular Medicine, Taipei Medical University-Wan Fang Hospital, Taipei, Taiwan

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CHEN-CHUAN CHENG M.D.,

CHEN-CHUAN CHENG M.D.

Division of Cardiology, Chi-Mei Medical Center, Tainan, Taiwan

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SATOSHI HIGA M.D., Ph.D.,

SATOSHI HIGA M.D., Ph.D.

Second Department of Internal Medicine, University of the Ryukyus, Okinawa, Japan

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YI-JEN CHEN M.D., Ph.D.,

YI-JEN CHEN M.D., Ph.D.

Division of Cardiovascular Medicine, Taipei Medical University-Wan Fang Hospital, Taipei, Taiwan

Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei, Taiwan

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SHIH-ANN CHEN M.D.,

SHIH-ANN CHEN M.D.

School of Medicine, National Yang-Ming University, Taipei, Taiwan

Division of Cardiology and Cardiovascular Research Center, Veterans General Hospital-Taipei, Taipei, Taiwan

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YAO-CHANG CHEN M.Sc.,

YAO-CHANG CHEN M.Sc.

Department of Biomedical Engineering, National Defense Medical Center, Taipei, Taiwan

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NAN-HUNG PAN M.D.,

NAN-HUNG PAN M.D.

Division of Cardiovascular Medicine, Taipei Medical University-Wan Fang Hospital, Taipei, Taiwan

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CHEN-CHUAN CHENG M.D.,

CHEN-CHUAN CHENG M.D.

Division of Cardiology, Chi-Mei Medical Center, Tainan, Taiwan

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SATOSHI HIGA M.D., Ph.D.,

SATOSHI HIGA M.D., Ph.D.

Second Department of Internal Medicine, University of the Ryukyus, Okinawa, Japan

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YI-JEN CHEN M.D., Ph.D.,

YI-JEN CHEN M.D., Ph.D.

Division of Cardiovascular Medicine, Taipei Medical University-Wan Fang Hospital, Taipei, Taiwan

Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei, Taiwan

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SHIH-ANN CHEN M.D.,

SHIH-ANN CHEN M.D.

School of Medicine, National Yang-Ming University, Taipei, Taiwan

Division of Cardiology and Cardiovascular Research Center, Veterans General Hospital-Taipei, Taipei, Taiwan

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First published: 26 August 2009

https://doi.org/10.1111/j.1540-8167.2009.01480.x

Citations: 39

Address for correspondence: Yi-Jen Chen, M.D., Ph.D., Division of Cardiovascular Medicine, Taipei Medical University-Wan Fang Hospital, 111 Hsin-Lung Road, Sec. 3, Taipei, Taiwan. Fax: +886-2-29339378; +886-2-28735656; E-mail: [email protected]

The present work was supported by the Center of Excellence for Clinical Trial and Research in Wan Fang Hospital (DOH-TD-B-111-002) and grants NSC 95-2314-B-016-015, NSC 95-2314-B-038-026, NSC 96-2628-B-038-012-MY3, NSC 96-2314-B-010-006, V95A-008, 96CM-TMU-07.

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Abstract

Introduction: The relationship between the determining factors and beating rates of pulmonary vein (PV) pacemaker cardiomyocytes has not been fully elucidated. The purposes of this study were to compare the electrophysiological characteristics between PV fast and slow pacemaker cardiomyocytes.

Methods: Whole-cell patch clamp was used to investigate the action potentials, transient outward currents (I_to), sustained outward potassium currents (I_Ksus), rapid delayed rectifier potassium currents (I_Kr), inward rectifier potassium current (I_K1), pacemaker currents (I_f), and transient inward currents in isolated rabbit PV single fast (≥2.5 Hz) and slow (<2.5 Hz) pacemaker cardiomyocytes.

Results: The fast PV pacemaker cardiomyocytes (n = 66) had lesser negative maximum diastolic potential (−53 ± 1 mV vs −59 ± 1 mV, P < 0.001) and larger slope of diastolic depolarization (0.31 ± 0.02 V/sec vs 0.09 ± 0.01 V/sec, P < 0.001) than slow PV pacemaker cardiomyocytes (n = 65). Moreover, the PV beating rates correlated well with the slope of diastolic depolarization and maximum diastolic potential with a linear regression. As compared to those in slow PV pacemaker cardiomyocytes, the fast PV pacemaker cardiomyocytes had smaller I_to and I_K1, and larger I_f but with similar I_Ksus,I_Kr, and transient inward currents. However, only few fast (2/34) and slow (1/23) PV pacemaker cardiomyocytes contained barium-sensitive hyperpolarization-activated time-dependent current (I_KH)_.

Conclusions and Implications: The fast and slow PV pacemaker had different electrophysiological characteristics. I_to, I_K1, and I_f may regulate the beating rates of PV pacemaker cardiomyocytes.

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Volume20, Issue9

September 2009

Pages 1039-1045

Heterogeneous Expression of Potassium Currents and Pacemaker Currents Potentially Regulates Arrhythmogenesis of Pulmonary Vein Cardiomyocytes

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Heterogeneous Expression of Potassium Currents and Pacemaker Currents Potentially Regulates Arrhythmogenesis of Pulmonary Vein Cardiomyocytes

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