Platelet concentrates from fresh or overnight-stored blood, an international study
M.J. Dijkstra-Tiekstra
From Sanquin Research, Groningen, the Netherlands; Sanquin Blood Supply, Amsterdam, the Netherlands; NHS Blood & Transplant England, Essex, UK; NHS National Services Scotland, Edinburgh, UK; Canadian Blood Services, Vancouver, British Columbia, Canada; and Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.
Search for more papers by this authorP.F. van der Meer
From Sanquin Research, Groningen, the Netherlands; Sanquin Blood Supply, Amsterdam, the Netherlands; NHS Blood & Transplant England, Essex, UK; NHS National Services Scotland, Edinburgh, UK; Canadian Blood Services, Vancouver, British Columbia, Canada; and Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.
Search for more papers by this authorR. Cardigan
From Sanquin Research, Groningen, the Netherlands; Sanquin Blood Supply, Amsterdam, the Netherlands; NHS Blood & Transplant England, Essex, UK; NHS National Services Scotland, Edinburgh, UK; Canadian Blood Services, Vancouver, British Columbia, Canada; and Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.
Search for more papers by this authorD. Devine
From Sanquin Research, Groningen, the Netherlands; Sanquin Blood Supply, Amsterdam, the Netherlands; NHS Blood & Transplant England, Essex, UK; NHS National Services Scotland, Edinburgh, UK; Canadian Blood Services, Vancouver, British Columbia, Canada; and Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.
Search for more papers by this authorC. Prowse
From Sanquin Research, Groningen, the Netherlands; Sanquin Blood Supply, Amsterdam, the Netherlands; NHS Blood & Transplant England, Essex, UK; NHS National Services Scotland, Edinburgh, UK; Canadian Blood Services, Vancouver, British Columbia, Canada; and Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.
Search for more papers by this authorP. Sandgren
From Sanquin Research, Groningen, the Netherlands; Sanquin Blood Supply, Amsterdam, the Netherlands; NHS Blood & Transplant England, Essex, UK; NHS National Services Scotland, Edinburgh, UK; Canadian Blood Services, Vancouver, British Columbia, Canada; and Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.
Search for more papers by this authorJ. de Wildt-Eggen
From Sanquin Research, Groningen, the Netherlands; Sanquin Blood Supply, Amsterdam, the Netherlands; NHS Blood & Transplant England, Essex, UK; NHS National Services Scotland, Edinburgh, UK; Canadian Blood Services, Vancouver, British Columbia, Canada; and Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.
Search for more papers by this authorfor the Biomedical Excellence for Safer Transfusion Collaborative
Search for more papers by this authorM.J. Dijkstra-Tiekstra
From Sanquin Research, Groningen, the Netherlands; Sanquin Blood Supply, Amsterdam, the Netherlands; NHS Blood & Transplant England, Essex, UK; NHS National Services Scotland, Edinburgh, UK; Canadian Blood Services, Vancouver, British Columbia, Canada; and Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.
Search for more papers by this authorP.F. van der Meer
From Sanquin Research, Groningen, the Netherlands; Sanquin Blood Supply, Amsterdam, the Netherlands; NHS Blood & Transplant England, Essex, UK; NHS National Services Scotland, Edinburgh, UK; Canadian Blood Services, Vancouver, British Columbia, Canada; and Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.
Search for more papers by this authorR. Cardigan
From Sanquin Research, Groningen, the Netherlands; Sanquin Blood Supply, Amsterdam, the Netherlands; NHS Blood & Transplant England, Essex, UK; NHS National Services Scotland, Edinburgh, UK; Canadian Blood Services, Vancouver, British Columbia, Canada; and Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.
Search for more papers by this authorD. Devine
From Sanquin Research, Groningen, the Netherlands; Sanquin Blood Supply, Amsterdam, the Netherlands; NHS Blood & Transplant England, Essex, UK; NHS National Services Scotland, Edinburgh, UK; Canadian Blood Services, Vancouver, British Columbia, Canada; and Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.
Search for more papers by this authorC. Prowse
From Sanquin Research, Groningen, the Netherlands; Sanquin Blood Supply, Amsterdam, the Netherlands; NHS Blood & Transplant England, Essex, UK; NHS National Services Scotland, Edinburgh, UK; Canadian Blood Services, Vancouver, British Columbia, Canada; and Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.
Search for more papers by this authorP. Sandgren
From Sanquin Research, Groningen, the Netherlands; Sanquin Blood Supply, Amsterdam, the Netherlands; NHS Blood & Transplant England, Essex, UK; NHS National Services Scotland, Edinburgh, UK; Canadian Blood Services, Vancouver, British Columbia, Canada; and Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.
Search for more papers by this authorJ. de Wildt-Eggen
From Sanquin Research, Groningen, the Netherlands; Sanquin Blood Supply, Amsterdam, the Netherlands; NHS Blood & Transplant England, Essex, UK; NHS National Services Scotland, Edinburgh, UK; Canadian Blood Services, Vancouver, British Columbia, Canada; and Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden.
Search for more papers by this authorfor the Biomedical Excellence for Safer Transfusion Collaborative
Search for more papers by this authorAbstract
BACKGROUND: Whole blood and also buffy coats (BCs) can be held for a few hours or overnight before processing into blood components or platelet concentrates (PCs). Individual studies have reported a range of outcomes regarding in vitro variables for PCs prepared from fresh and stored whole blood. In this multicenter study, effects of storage of whole blood or BCs on the in vitro quality of PCs were studied.
STUDY DESIGN AND METHODS: The leukoreduced BC PCs were prepared from fresh BCs (2-8 hr after collection; fresh/fresh), from BCs at 20 to 24 hours after collection (fresh/stored), or from BCs prepared from whole blood stored for 20 to 24 hours (stored/fresh). PCs were stored on a flat-bed shaker at 20 to 24°C for 7 days. PCs were tested on Days 0 (only fresh/fresh), 1, 5, and 7 for in vitro quality. There were six participating centers that tested all three conditions with n = 6 per condition.
RESULTS: In comparison to fresh/stored and stored/fresh PCs, fresh/fresh PCs exhibited a lower platelet (PLT) count (Day 1—220 × 109 ± 70 × 109 vs. 324 × 109 ± 50 × 109 and 368 × 109 ± 56 × 109 PLTs/PC), lactate, pCO2, and hypotonic shock response (HSR; Days 5 and 7; Day 7—50 ± 13% vs. 57 ± 12 and 63 ± 11%) and a higher pH, glucose, pO2, and CD62P expression (than stored/fresh PCs only; Day 7—33 ± 10% vs. 28 ± 12 and 24 ± 11%; p < 0.05). No differences were observed for volume, swirling effect, white blood cell count, annexin V binding, or aggregation between these conditions.
CONCLUSION: Based on PLT count, HSR, and PLT activation, PCs are best prepared after 20 to 24 hours hold of the whole blood or BCs.
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