Evaluation of the properties of components prepared and stored after holding of whole blood units for 8 and 24 hours at ambient temperature
Gary Moroff
From the American Red Cross, Holland Laboratory, Rockville, Maryland; the Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire; the American Red Cross, Mid-Atlantic Region, Norfolk, Virginia.
Search for more papers by this authorJames P. AuBuchon
From the American Red Cross, Holland Laboratory, Rockville, Maryland; the Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire; the American Red Cross, Mid-Atlantic Region, Norfolk, Virginia.
Search for more papers by this authorConstance Pickard
From the American Red Cross, Holland Laboratory, Rockville, Maryland; the Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire; the American Red Cross, Mid-Atlantic Region, Norfolk, Virginia.
Search for more papers by this authorPamela H. Whitley
From the American Red Cross, Holland Laboratory, Rockville, Maryland; the Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire; the American Red Cross, Mid-Atlantic Region, Norfolk, Virginia.
Search for more papers by this authorW. Andrew Heaton
From the American Red Cross, Holland Laboratory, Rockville, Maryland; the Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire; the American Red Cross, Mid-Atlantic Region, Norfolk, Virginia.
Search for more papers by this authorStein Holme
From the American Red Cross, Holland Laboratory, Rockville, Maryland; the Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire; the American Red Cross, Mid-Atlantic Region, Norfolk, Virginia.
Search for more papers by this authorGary Moroff
From the American Red Cross, Holland Laboratory, Rockville, Maryland; the Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire; the American Red Cross, Mid-Atlantic Region, Norfolk, Virginia.
Search for more papers by this authorJames P. AuBuchon
From the American Red Cross, Holland Laboratory, Rockville, Maryland; the Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire; the American Red Cross, Mid-Atlantic Region, Norfolk, Virginia.
Search for more papers by this authorConstance Pickard
From the American Red Cross, Holland Laboratory, Rockville, Maryland; the Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire; the American Red Cross, Mid-Atlantic Region, Norfolk, Virginia.
Search for more papers by this authorPamela H. Whitley
From the American Red Cross, Holland Laboratory, Rockville, Maryland; the Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire; the American Red Cross, Mid-Atlantic Region, Norfolk, Virginia.
Search for more papers by this authorW. Andrew Heaton
From the American Red Cross, Holland Laboratory, Rockville, Maryland; the Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire; the American Red Cross, Mid-Atlantic Region, Norfolk, Virginia.
Search for more papers by this authorStein Holme
From the American Red Cross, Holland Laboratory, Rockville, Maryland; the Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire; the American Red Cross, Mid-Atlantic Region, Norfolk, Virginia.
Search for more papers by this authorAbstract
BACKGROUND: The capability of holding whole blood (WB) units at ambient temperature, overnight, should help in platelet (PLT) concentrate preparation logistics. We summarize the results of a study conducted in the early 1990s that compared, in particular, PLT and red blood cell (RBC) in vivo viability properties following storage after preparation after 8- and 24-hour WB hold periods.
STUDY DESIGN AND METHODS: Individuals donated units of WB on two occasions. Centrifugation at 20 to 24°C to separate PLTs and additive system RBC placement at 1 to 6°C was completed 8 hours after phlebotomy or after 24 hours in randomized order. Components were not leukoreduced. Studies including in vitro biochemical and hematologic analyses and autologous in vivo RBC and PLT evaluations were conducted at two sites.
RESULTS: RBC 24-hour in vivo (mean ± SD) recoveries (single-label approach), after 35 days of storage, were 79.2 ± 4.3 and 79.4 ± 3.9% (n = 9; p > 0.05), with WB holding periods of 8 and 24 hours, respectively. With 42 days of storage, recovery after a 24-hour hold was slightly less than with an 8-hour hold (72.9 ± 6.5% vs. 76.0 ± 5.4%; n = 17; p < 0.05). RBC 2,3-diphosphoglycerate acid levels were substantially less after the 24-hour hold compared to after the 8-hour hold (n = 18; p < 0.05). PLT recovery after 5 days of storage with 8- and 24-hour hold periods were similar, 51.1 ± 14.9 and 50.6 ± 17.7%, respectively (n = 18; p > 0.05). The PLT survival variable and in vitro properties reflecting storage quality also showed no significant difference.
CONCLUSION: RBC and PLT in vivo variables, and most in vitro variables, were not significantly different after storage with WB holding times of 8 and 24 hours except for a slight diminution of RBC recovery with the 24-hour hold after 42 days of storage.
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