ORIGINAL ARTICLE
Na+/K+-ATPase inhibition upregulates NMDA-evoked currents in rat hippocampal CA1 pyramidal neurons
Linan Zhang,
Fang Guo,
Suwen Su,
Huicai Guo,
Chen Xiong,
Jian Yin,
Wenya Li,
Yongli Wang,
Linan Zhang
Department of Pharmacology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, Hebei 050017, China
Pharmacy Department, Hebei University of Science and Technology, 70 Yuhua East Road, Shijiazhuang, Hebei 050018, China
Search for more papers by this authorFang Guo
Department of Pharmacology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, Hebei 050017, China
Search for more papers by this authorSuwen Su
Department of Pharmacology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, Hebei 050017, China
Search for more papers by this authorHuicai Guo
Department of Toxicology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, Hebei 050017, China
Search for more papers by this authorChen Xiong
Department of Pharmacology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, Hebei 050017, China
Search for more papers by this authorJian Yin
Department of Pharmacology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, Hebei 050017, China
Search for more papers by this authorWenya Li
Department of Pharmacology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, Hebei 050017, China
Search for more papers by this authorCorresponding Author
Yongli Wang
Department of Pharmacology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, Hebei 050017, China
Correspondence and reprints: [email protected]; [email protected]Search for more papers by this authorLinan Zhang,
Fang Guo,
Suwen Su,
Huicai Guo,
Chen Xiong,
Jian Yin,
Wenya Li,
Yongli Wang,
Linan Zhang
Department of Pharmacology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, Hebei 050017, China
Pharmacy Department, Hebei University of Science and Technology, 70 Yuhua East Road, Shijiazhuang, Hebei 050018, China
Search for more papers by this authorFang Guo
Department of Pharmacology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, Hebei 050017, China
Search for more papers by this authorSuwen Su
Department of Pharmacology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, Hebei 050017, China
Search for more papers by this authorHuicai Guo
Department of Toxicology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, Hebei 050017, China
Search for more papers by this authorChen Xiong
Department of Pharmacology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, Hebei 050017, China
Search for more papers by this authorJian Yin
Department of Pharmacology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, Hebei 050017, China
Search for more papers by this authorWenya Li
Department of Pharmacology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, Hebei 050017, China
Search for more papers by this authorCorresponding Author
Yongli Wang
Department of Pharmacology, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, Hebei 050017, China
Correspondence and reprints: [email protected]; [email protected]Search for more papers by this authorAbstract
Na+/K+-ATPase and N-methyl-d-aspartate (NMDA) receptor in hippocampus play very important roles in the regulation of learning and memory. Here, we showed that dihydroouabain (DHO, 10−5–10−3 m), a Na+/K+-ATPase inhibitor, significantly potentiated NMDA current in rat hippocampal CA1 pyramidal neurons, which was blocked by PP2 (the selective Src tyrosine kinase inhibitor) and PD-98059 [the selective inhibitor of the mitogen-activated protein kinases (MAPK) cascade]. These findings reported here uncover that Src mediates the cross-talk between Na+/K+-ATPase and NMDA receptor to transduce the signals from Na+/K+-ATPase to the MAPK cascade and provide new insights into therapeutic target for deeper understanding of the nature of cognitive disorder.
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