Colin Harbour, Department of Infectious Diseases and Immunology, Blackburn Building DO6, University of Sydney, Sydney, NSW 2006, Australia. Email: [email protected]Search for more papers by this author

Pholawat TINGPEJ,

Pholawat TINGPEJ

Departments of Infectious Diseases and Immunology and

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Mark ELKINS,

Mark ELKINS

Departments of Respiratory Medicine and

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Barbara ROSE,

Barbara ROSE

Departments of Infectious Diseases and Immunology and

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Honghua HU,

Honghua HU

Departments of Infectious Diseases and Immunology and

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Carmel MORIARTY,

Carmel MORIARTY

Departments of Respiratory Medicine and

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Jim MANOS,

Jim MANOS

Departments of Infectious Diseases and Immunology and

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Belinda BARRAS,

Belinda BARRAS

Microbiology, Royal Prince Alfred Hospital, Sydney, New South Wales, Australia

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Peter BYE,

Peter BYE

Departments of Respiratory Medicine and

Medicine, University of Sydney, and

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Colin HARBOUR,

Corresponding Author

Colin HARBOUR

Departments of Infectious Diseases and Immunology and

First published: 21 July 2010

https://doi.org/10.1111/j.1440-1843.2010.01792.x

Citations: 19

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ABSTRACT

Background and objective: Earlier reports suggested that Pseudomonas aeruginosa frequent epidemic clones circulating in cystic fibrosis (CF) centres had increased virulence. However, recent data show no consistent associations with virulence, and suggest attenuation of virulence in chronic infection. Changes to infection control programmes in relation to frequent epidemic clones should be based on their frequency, virulence across all age groups and mode of acquisition. The Australian epidemic strain-1 (AES-1) (or the Melbourne epidemic strain) and AES-2 are common in CF clinics in mainland eastern Australia, but not in the environment. Both have shown increased virulence, but there are no data specifically in adults. This study examines the frequency and virulence of P. aeruginosa frequent epidemic clones in the adult CF clinic at Royal Prince Alfred Hospital, Sydney, Australia.

Methods: Two hundred and fifty-eight P. aeruginosa isolates from 112 participants were genotyped by pulsed field gel electrophoresis. Ninety-eight patients were followed up for 1 year and associations sought between infection with a frequent epidemic clone, clinical outcome and antibiotic resistance.

Results: Four frequent P. aeruginosa epidemic clones (AES-1, AES-2, S-1, S-2) affected almost 50% of participants. AES-1 predominated (38%). AES-1, AES-2 and S-1 were associated with increased exacerbations and hospital-admission days. AES-1 showed increased resistance to aminoglycosides and ticarcillin-clavulanate.

Conclusions: This study supports the potential threat of frequent P. aeruginosa epidemic clones in adult CF populations.

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Volume15, Issue6

August 2010

Pages 923-929

Clinical profile of adult cystic fibrosis patients with frequent epidemic clones of Pseudomonas aeruginosa

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Clinical profile of adult cystic fibrosis patients with frequent epidemic clones of Pseudomonas aeruginosa

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