Identification of novel genes expressed during rhabdomyosarcoma differentiation using cDNA microarrays
David Segal
Metabolic Research Unit, School of Exercise and Nutrition Sciences, Deakin University, Melbourne and
Search for more papers by this authorReuben Klein
Metabolic Research Unit, School of Exercise and Nutrition Sciences, Deakin University, Melbourne and
Search for more papers by this authorAndrew Sanigorski
Metabolic Research Unit, School of Exercise and Nutrition Sciences, Deakin University, Melbourne and
Search for more papers by this authorKen Walder
Metabolic Research Unit, School of Exercise and Nutrition Sciences, Deakin University, Melbourne and
ChemGenex Pharmaceuticals, Geelong, Victoria, Australia
Search for more papers by this authorGregory R. Collier
Metabolic Research Unit, School of Exercise and Nutrition Sciences, Deakin University, Melbourne and
ChemGenex Pharmaceuticals, Geelong, Victoria, Australia
Search for more papers by this authorCorresponding Author
David Cameron-Smith
Center for Physical Activity and Nutrition and
David Cameron-Smith, PhD, School of Exercise and Nutrition Sciences, Deakin University, 221 Burwood Highway, Burwood, Victoria 3125, Australia. Email: [email protected]Search for more papers by this authorDavid Segal
Metabolic Research Unit, School of Exercise and Nutrition Sciences, Deakin University, Melbourne and
Search for more papers by this authorReuben Klein
Metabolic Research Unit, School of Exercise and Nutrition Sciences, Deakin University, Melbourne and
Search for more papers by this authorAndrew Sanigorski
Metabolic Research Unit, School of Exercise and Nutrition Sciences, Deakin University, Melbourne and
Search for more papers by this authorKen Walder
Metabolic Research Unit, School of Exercise and Nutrition Sciences, Deakin University, Melbourne and
ChemGenex Pharmaceuticals, Geelong, Victoria, Australia
Search for more papers by this authorGregory R. Collier
Metabolic Research Unit, School of Exercise and Nutrition Sciences, Deakin University, Melbourne and
ChemGenex Pharmaceuticals, Geelong, Victoria, Australia
Search for more papers by this authorCorresponding Author
David Cameron-Smith
Center for Physical Activity and Nutrition and
David Cameron-Smith, PhD, School of Exercise and Nutrition Sciences, Deakin University, 221 Burwood Highway, Burwood, Victoria 3125, Australia. Email: [email protected]Search for more papers by this authorAbstract
Rhabdomyosarcomas (RMS) are highly aggressive tumors that are thought to arise as a consequence of the regulatory disruption of the growth and differentiation of skeletal muscle progenitor cells. Normal myogenesis is characterized by the expression of the myogenic regulatory factor gene family but, despite their expression in RMS, these tumor cells fail to complete the latter stages of myogenesis. The RMS cell line RD-A was treated with 12-O-tetradecanoylphorbol-13-acetate to induce differentiation and cultured for 10 days. RNA was extracted on days 1, 3, 6, 8 and 10. A human skeletal muscle cDNA microarray was developed and used to analyze the global gene expression of RMS tumors over the time-course of differentiation. As a comparison, the genes identified were subsequently examined during the differentiated primary human skeletal muscle cultures. Prothymosin alpha (PTMA), and translocase of inner mitochondrial membrane 10 (Tim10), two genes not previously implicated in RMS, showed reduced expression during differentiation. Marked differences in the expression of PTMA and Tim10 were observed during the differentiation of human primary skeletal muscle cells. These results identify several new genes with potential roles in the myogenic arrest present in rhabdomyosarcoma. PTMA expression in RMS biopsy samples might prove to be an effective diagnostic marker for this disease.
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