Comparison of cryopreserved human sperm from solid surface vitrification and standard vapor freezing method: on motility, morphology, vitality and DNA integrity
Corresponding Author
C. Satirapod
Department of Obstetrics and Gynaecology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand;
Correspondence
Dr. Chonthicha Satirapod, Department of Obstetrics and Gynaecology, Faculty of Medicine, Ramathibodi Hospital, 270 Rama 6 Rd., Ratchatawee, Bangkok 10400, Thailand.
Tel.: +66 220 11412;
Fax: +66 220 11416;
E-mail: [email protected]
Search for more papers by this authorC. Treetampinich
Department of Obstetrics and Gynaecology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand;
Search for more papers by this authorS. Weerakiet
Department of Obstetrics and Gynaecology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand;
Search for more papers by this authorA. Wongkularb
Department of Obstetrics and Gynaecology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand;
Search for more papers by this authorS. Rattanasiri
Faculty of Medicine, Research Center, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
Search for more papers by this authorW. Choktanasiri
Department of Obstetrics and Gynaecology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand;
Search for more papers by this authorCorresponding Author
C. Satirapod
Department of Obstetrics and Gynaecology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand;
Correspondence
Dr. Chonthicha Satirapod, Department of Obstetrics and Gynaecology, Faculty of Medicine, Ramathibodi Hospital, 270 Rama 6 Rd., Ratchatawee, Bangkok 10400, Thailand.
Tel.: +66 220 11412;
Fax: +66 220 11416;
E-mail: [email protected]
Search for more papers by this authorC. Treetampinich
Department of Obstetrics and Gynaecology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand;
Search for more papers by this authorS. Weerakiet
Department of Obstetrics and Gynaecology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand;
Search for more papers by this authorA. Wongkularb
Department of Obstetrics and Gynaecology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand;
Search for more papers by this authorS. Rattanasiri
Faculty of Medicine, Research Center, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
Search for more papers by this authorW. Choktanasiri
Department of Obstetrics and Gynaecology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand;
Search for more papers by this authorSummary
Solid surface vitrificaition (SSV) is a cryoperservative method that has been used in the cryopreservation of oocytes, and embryos. Here, we report an application of the SSV in the cryopreservation of human spermatozoa. We compared the SSV with a standard freezing method in terms of sperm motility, morphology, vitality and DNA integrity. Sperm motility was determined by computer assisted semen analysis, morphology and vitality were determined by eosin-methylene blue staining, and DNA integrity was determined by a TUNEL assay. We found that while both cryopreservative methods produced spermatozoa with comparable vitality and motility, the SSV gave slightly, but significantly fewer sperm with DNA damage, and loose tail. We concluded that, a cryopreservation of human spermatozoa by SSV is feasible and provides a quick and practical way to preserve human spermatozoa with a comparable, if not better, quality of the preserved spermatozoa to the standard freezing method.
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