Volume 36, Issue 8 pp. 993-1000

Associations between antioxidant status, markers of oxidative stress and immune responses in allergic adults

Correction(s) for this article

J. A. Dunstan,

J. A. Dunstan

School of Paediatrics and Child Health, University of Western Australia, Perth, WA, Australia,

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L. Breckler,

L. Breckler

School of Paediatrics and Child Health, University of Western Australia, Perth, WA, Australia,

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J. Hale,

J. Hale

School of Paediatrics and Child Health, University of Western Australia, Perth, WA, Australia,

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H. Lehmann,

H. Lehmann

School of Paediatrics and Child Health, University of Western Australia, Perth, WA, Australia,

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P. Franklin,

P. Franklin

School of Paediatrics and Child Health, University of Western Australia, Perth, WA, Australia,

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G. Lyonso,

G. Lyonso

School of Agriculture & Wine, University of Adelaide – Waite Campus, Glen Osmond, SA, Australia,

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S. Y. L. Ching,

S. Y. L. Ching

Biochemistry Department, Clinical Pathology, PathWest, Perth, WA, Australia

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T. A. Mori,

T. A. Mori

School of Medicine and Pharmacology, University of Western Australia, Perth, WA, Australia.

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A. Barden,

A. Barden

School of Medicine and Pharmacology, University of Western Australia, Perth, WA, Australia.

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S. L. Prescott,

S. L. Prescott

School of Paediatrics and Child Health, University of Western Australia, Perth, WA, Australia,

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J. A. Dunstan,

J. A. Dunstan

School of Paediatrics and Child Health, University of Western Australia, Perth, WA, Australia,

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L. Breckler,

L. Breckler

School of Paediatrics and Child Health, University of Western Australia, Perth, WA, Australia,

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J. Hale,

J. Hale

School of Paediatrics and Child Health, University of Western Australia, Perth, WA, Australia,

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H. Lehmann,

H. Lehmann

School of Paediatrics and Child Health, University of Western Australia, Perth, WA, Australia,

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P. Franklin,

P. Franklin

School of Paediatrics and Child Health, University of Western Australia, Perth, WA, Australia,

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G. Lyonso,

G. Lyonso

School of Agriculture & Wine, University of Adelaide – Waite Campus, Glen Osmond, SA, Australia,

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S. Y. L. Ching,

S. Y. L. Ching

Biochemistry Department, Clinical Pathology, PathWest, Perth, WA, Australia

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T. A. Mori,

T. A. Mori

School of Medicine and Pharmacology, University of Western Australia, Perth, WA, Australia.

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A. Barden,

A. Barden

School of Medicine and Pharmacology, University of Western Australia, Perth, WA, Australia.

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S. L. Prescott,

S. L. Prescott

School of Paediatrics and Child Health, University of Western Australia, Perth, WA, Australia,

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First published: 01 August 2006

https://doi.org/10.1111/j.1365-2222.2006.02539.x

Citations: 28

Correspondence:
Susan L. Prescott, Department of Paediatrics, Princess Margaret Hospital, Perth, PO Box D184, WA 6001, Australia. E-mail: [email protected]

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Summary

Background There has been growing interest in the role of antioxidant function in controlling inflammatory disease states, such as allergy. This study investigated the relationship between antioxidant status, markers of airways inflammation [exhaled nitric oxide (eNO)], oxidative stress (F₂ isoprostanes) and immune responses in allergic adults.

Methods Antioxidants (vitamins C, E, β-carotene and selenium) and total antioxidant capacity (tAC) in serum were examined in relation to eNO, plasma F₂ isoprostanes and peripheral blood mononuclear cell (PBMC) cytokine and lymphoproliferative response to house dust mite (HDM) allergen, Staphylococcus enterotoxin B (SEB), phytohaemaglutinin (PHA) and lipopolysaccharide (LPS) in 54 allergic adults.

Results Firstly, levels of specific vitamins did not correlate with tAC. Secondly, we did not see any evidence that specific vitamin levels (or tAC) were associated with either polarization or attenuation of in vitro immune responses. If anything, there were positive correlations between antioxidant (vitamin C and selenium) levels and HDM allergen responses [lymphoproliferation (selenium; r=0.35, P=0.013) and both Th2 IL13 (vitamin C; τ=0.254, P=0.028) and Th1 IFN-γ (vitamin C; τ=0.302, P=0.009) responses]. There were also significant positive relationships between antioxidant levels and IL-10 responses to polyclonal stimulation by SEB (r=0.292, P=0.036) and LPS (r=0.34, P=0.015) (β-carotene) and PHA (r=0.34, P=0.021) (tAC). Thirdly, although airways inflammation (eNO) was associated with both in vitro and in vivo (skin test reactivity) to HDM, we did not see any correlation between eNO and oxidative stress (F₂-isoprostanes). Finally, there were no consistent relationships between oxidative stress and immune responses.

Conclusion There was no evidence that higher antioxidant levels were associated with reduced allergen responsiveness in allergic adults. If anything, antioxidant status was associated with increased immune responsiveness. The significance of this needs to be addressed in future intervention studies.

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Volume36, Issue8

August 2006

Pages 993-1000

Associations between antioxidant status, markers of oxidative stress and immune responses in allergic adults

Correction(s) for this article

Associations between antioxidant status, markers of oxidative stress and immune responses in allergic adults

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Associations between antioxidant status, markers of oxidative stress and immune responses in allergic adults

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Associations between antioxidant status, markers of oxidative stress and immune responses in allergic adults

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