Role of the interaction between Lu/BCAM and the spectrin-based membrane skeleton in the increased adhesion of hereditary spherocytosis red cells to laminin
Emilie Gauthier
Inserm, UMR-S 665
Université Paris Diderot-Paris 7
Institut National de la Transfusion Sanguine
E.G. and W. EN equally contributed to this work.
Search for more papers by this authorWassim El Nemer
Inserm, UMR-S 665
Université Paris Diderot-Paris 7
Institut National de la Transfusion Sanguine
E.G. and W. EN equally contributed to this work.
Search for more papers by this authorMarie P. Wautier
Inserm, UMR-S 665
Université Paris Diderot-Paris 7
Institut National de la Transfusion Sanguine
Search for more papers by this authorJean Delaunay
INSERM U 779, Faculté de Médecine Paris-Sud, Université Paris-Sud, Le Kremlin-Bicêtre, France
Search for more papers by this authorCaroline Le Van Kim
Inserm, UMR-S 665
Université Paris Diderot-Paris 7
Institut National de la Transfusion Sanguine
Search for more papers by this authorYves Colin
Inserm, UMR-S 665
Université Paris Diderot-Paris 7
Institut National de la Transfusion Sanguine
Search for more papers by this authorEmilie Gauthier
Inserm, UMR-S 665
Université Paris Diderot-Paris 7
Institut National de la Transfusion Sanguine
E.G. and W. EN equally contributed to this work.
Search for more papers by this authorWassim El Nemer
Inserm, UMR-S 665
Université Paris Diderot-Paris 7
Institut National de la Transfusion Sanguine
E.G. and W. EN equally contributed to this work.
Search for more papers by this authorMarie P. Wautier
Inserm, UMR-S 665
Université Paris Diderot-Paris 7
Institut National de la Transfusion Sanguine
Search for more papers by this authorJean Delaunay
INSERM U 779, Faculté de Médecine Paris-Sud, Université Paris-Sud, Le Kremlin-Bicêtre, France
Search for more papers by this authorCaroline Le Van Kim
Inserm, UMR-S 665
Université Paris Diderot-Paris 7
Institut National de la Transfusion Sanguine
Search for more papers by this authorYves Colin
Inserm, UMR-S 665
Université Paris Diderot-Paris 7
Institut National de la Transfusion Sanguine
Search for more papers by this authorPresented in part in an abstract (Hematology Meeting Reports 2008, vol. 2; no.4) at the Red Cell and Iron Club Conference, 2008, Brugge, Belgium.
Summary
Lu/BCAM, the unique erythroid receptor for laminin 511/521, interacts with the erythrocyte membrane skeleton through spectrin binding. It has been reported that Hereditary Spherocytosis red blood cells (HS RBC) exhibit increased adhesion to laminin. We investigated the role of Lu/BCAM–spectrin interaction in the RBC adhesion properties of 2 splenectomised HS patients characterized by 40% spectrin deficiency. Under physiological flow conditions, HS RBC exhibited an exaggerated adhesion to laminin that was completely abolished by soluble Lu/BCAM. Triton extraction experiments revealed that a greater fraction of Lu/BCAM was unlinked to the membrane skeleton of HS RBC, as compared to normal RBC. Disruption of the spectrin interaction site in Lu/BCAM expressed in the transfected K562 cell line resulted in a weakened interaction to the skeleton and an enhanced interaction to laminin. These results demonstrated that the adhesion of HS RBC to laminin was mediated by Lu/BCAM and that its interaction with the spectrin-based skeleton negatively regulated cell adhesion to laminin. Finally, the results of this study strongly suggest that the reinforced adhesiveness of spectrin-deficient HS RBC to laminin is partly brought about by an impaired interaction between Lu/BCAM and the membrane skeleton.
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