Background Human hair follicle keratin-associated proteins (KAPs) comprise a large multigene family of proteins thought to be responsible for the bundling of keratin intermediate filaments. Recently, four new KAP family members KAP24.1, KAP25.1, KAP26.1 and KAP27.1 were identified from the genome, but the expression of only one, KAP24.1, was investigated and shown in hair follicles.

Objectives In the current study, the expression of the remaining members of the family were analysed.

Methods Reverse transcriptase-polymerase chain reaction analysis of samples from numerous human organs was used.

Results Only KAP26.1 showed expression, which was limited to the hair follicle. By in situ hybridization and immunohistochemistry using a specific antiserum, KAP26.1 was localized to the differentiated portion of the hair cuticle.

Conclusions As well as KAP24.1 in hair follicles, expression of KAP26.1 was shown and is found in the differentiated part of the hair cuticle.

References

1 Langbein L, Schweizer J. Keratins of the human hair follicle. Int Rev Cytol 2005; 243: 1–78.
10.1016/S0074-7696(05)43001-6
CAS PubMed Web of Science® Google Scholar
2 Rogers MA, Langbein L, Praetzel-Wunder S et al. Human hair keratin-associated proteins (KAPs). Int Rev Cytol 2006; 251: 209–63.
10.1016/S0074-7696(06)51006-X
CAS PubMed Web of Science® Google Scholar
3 Powell BC, Rogers GE. The role of keratin proteins and their genes in the growth, structure and properties of hair. In: Formation and Structure of Human Hair ( P Jolles, H Zahn, H Höcker, eds). Basel: Birkhäuser Verlag, 1997; 59–148.
10.1007/978-3-0348-9223-0_3
Google Scholar
4 Rogers MA, Langbein L, Winter H et al. Characterization of a cluster of human high/ultrahigh sulfur keratin associated protein (KAP) genes imbedded in the type I keratin gene domain on chromosome 17q12–21. J Biol Chem 2001; 276: 19440–51.
10.1074/jbc.M100657200
CAS PubMed Web of Science® Google Scholar
5 Rogers MA, Langbein L, Winter H et al. Characterization of a first domain of human high glycine-tyrosine and high sulfur keratin-associated protein (KAP) genes on chromosome 21q22.1. J Biol Chem 2002; 277: 48993–9002.
10.1074/jbc.M206422200
CAS PubMed Web of Science® Google Scholar
6 Rogers MA, Langbein L, Winter H et al. Hair keratin associated proteins: characterization of a second high sulfur KAP gene domain on human chromosome 21. J Invest Dermatol 2004; 122: 147–58.
10.1046/j.0022-202X.2003.22128.x
CAS PubMed Web of Science® Google Scholar
7 Yahagi S, Shibuya K, Obayashi I et al. Identification of two novel clusters of ultrahigh-sulfur keratin-associated protein genes on human chromosome 11. Biochem Biophys Res Commun 2004; 318: 655–64.
10.1016/j.bbrc.2004.04.074
CAS PubMed Web of Science® Google Scholar
8 Shibuya K, Obayashi I, Asakawa S et al. A cluster of 21 keratin-associated protein genes within introns of another gene on human chromosome 21q22.3. Genomics 2004; 83: 679–93.
10.1016/j.ygeno.2003.09.024
CAS PubMed Web of Science® Google Scholar
9 Soma T, Iino M, Tajima M et al. Expression of novel keratin associated protein 5 genes in the cuticle layer of human hair follicles. J Dermatol Sci 2005; 38: 110–12.
10.1016/j.jdermsci.2005.02.003
CAS PubMed Web of Science® Google Scholar
10 Rogers MA, Winter H, Langbein L et al. Characterization of human KAP24.1, a cuticular hair keratin-associated protein with unusual amino-acid composition and repeat structure. J Invest Dermatol 2007; 127: 1197–204.
10.1038/sj.jid.5700702
CAS PubMed Web of Science® Google Scholar
11 Dijkman BPPM, Mentzel S, De Jong AS et al. RNA in situ hybridization using digoxigenin-labeled cRNA probes. Biochemica 1995; 2: 21–5.
Google Scholar
12 MacKinnon PJ, Powell BC, Rogers GE. Structure and expression of genes for a class of cysteine-rich proteins of the cuticle layers of differentiating wool and hair follicles. J Cell Biol 1990; 111: 2587–600.
10.1083/jcb.111.6.2587
CAS PubMed Web of Science® Google Scholar
13 Swift JA. Morphology and biochemistry of human hair. In: Formation and Structure of Human Hair ( P Jolles, H Zahn, H Höcker, eds). Berlin: Birkhäuser Verlag, 1997; 149–76.
10.1007/978-3-0348-9223-0_4
Google Scholar
14 Rogers GE. Hair follicle differentiation and regulation. Int J Dev Biol 2004; 48: 163–70.
10.1387/ijdb.15272381
CAS PubMed Web of Science® Google Scholar
15 Jones LN, Rogers GE. Protein expression in developing wool fibre cuticle cells. In: Proceedings of the 11th Wool Research Conference ( JA Swift, K Byrne, P Duffield et al. , eds). Leeds: University of Leeds, 2006: CD-ROM.
Google Scholar

Citing Literature

Volume159, Issue3

September 2008

Pages 725-729

Characterization and expression analysis of the hair keratin associated protein KAP26.1

Summary

References

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

Characterization and expression analysis of the hair keratin associated protein KAP26.1

Summary

References

Citing Literature

References

Related

Information