International Journal of Laboratory Hematology

Volume 47, Issue 2 pp. 288-296

ORIGINAL ARTICLE

Identification of Potential Biomarkers and Pathways in Acute Myeloid Leukemia: Correlation Between the Calcineurin Signaling Pathway and Vascular Brittleness in Acute Myeloid Leukemia

Homood Alharbi,

Homood Alharbi

Department of Medical-Surgical Nursing, College of Nursing, King Saud University, Riyadh, Saudi Arabia

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Mohammad Ahmad,

Mohammad Ahmad

orcid.org/0000-0003-1228-4445

Department of Medical-Surgical Nursing, College of Nursing, King Saud University, Riyadh, Saudi Arabia

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Zhong Cui,

Zhong Cui

Department of Hematology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China

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Dong Meng,

Dong Meng

Department of Endocrinology and Metabolism, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China

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Ying Xin,

Ying Xin

Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China

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Xues Yan,

Corresponding Author

Xues Yan

[email protected]

Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China

Correspondence:

Xues Yan ([email protected])

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Homood Alharbi,

Homood Alharbi

Department of Medical-Surgical Nursing, College of Nursing, King Saud University, Riyadh, Saudi Arabia

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Mohammad Ahmad,

Mohammad Ahmad

orcid.org/0000-0003-1228-4445

Department of Medical-Surgical Nursing, College of Nursing, King Saud University, Riyadh, Saudi Arabia

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Zhong Cui,

Zhong Cui

Department of Hematology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China

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Dong Meng,

Dong Meng

Department of Endocrinology and Metabolism, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China

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Ying Xin,

Ying Xin

Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China

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Xues Yan,

Corresponding Author

Xues Yan

[email protected]

Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China

Correspondence:

Xues Yan ([email protected])

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First published: 05 December 2024

https://doi.org/10.1111/ijlh.14410

Funding: The authors received no specific funding for this work.

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ABSTRACT

Objective

In this study, clinical bioinformatics analysis was used to identify potential biomarkers of acute myeloid leukemia (AML) occurrence and development, drug resistance, and poor prognosis to provide a theoretical basis for the treatment of AML.

Methods

On the basis of the TCGA, GEO, and GTEx databases, an AML secondary database was established, and differential expression analysis and WGCNA were carried out to identify genes related to the prognosis of AML patients. Survival analysis was carried out for internal verification of key genes, and GEO data were used for external verification to obtain core genes related to prognosis. For differentially expressed genes, the EpiMed platform independently developed by the team was used for drug prediction.

Results

A total of 36 overlapping genes were obtained via difference analysis and WGCNA. Enrichment analysis revealed that the overlapping genes were associated with neutrophil activation, transcription dysregulation, AML, apoptosis, and other biological indicators. A protein interaction network was constructed for NCOA4, ACSL4, DPP4, ATL1, MT1G, ALOX15, and SLC7A11, which are key genes. Survival analysis revealed that NCOA4, ACSL4, DPP4, and ATL1 significantly affected the survival of patients with AML. The GSE142698 dataset verified that MPO, BCL2A1, and STMN1 had a statistically significant impact on the survival of AML patients.

Conclusion

NCOA4, ACSL4, DPP4, and ATL1 may be potential biomarkers related to the survival and prognosis of patients with AML, and the calcineurin signaling pathway is associated with the risk of vascular fragility in AML patients, which can provide a reference for further research and optimization of treatment regimens.

Conflicts of Interest

The authors declare no conflicts of interest.

Open Research

Data Availability Statement

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Supporting Information

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Citing Literature

Volume47, Issue2

April 2025

Pages 288-296

Filename	Description
ijlh14410-sup-0001-FigureS1.jpgJPEG image, 82.1 KB	Figure S1. PCR quantitative analysis results.
ijlh14410-sup-0002-FigureS2.jpgJPEG image, 168.4 KB	Figure S2. The results of the immunofluorescence assay.

Identification of Potential Biomarkers and Pathways in Acute Myeloid Leukemia: Correlation Between the Calcineurin Signaling Pathway and Vascular Brittleness in Acute Myeloid Leukemia

ABSTRACT

Objective

Methods

Results

Conclusion

Conflicts of Interest

Open Research

Data Availability Statement

Supporting Information

References

Citing Literature

References

Information

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Identification of Potential Biomarkers and Pathways in Acute Myeloid Leukemia: Correlation Between the Calcineurin Signaling Pathway and Vascular Brittleness in Acute Myeloid Leukemia

ABSTRACT

Objective

Methods

Results

Conclusion

Conflicts of Interest

Open Research

Data Availability Statement

Supporting Information

References

Citing Literature

References

Related

Information