Effect of preheating at 40℃ for 15 minutes to high-titer cold agglutinins on complete blood count
Hongxiang Xie
Department of Clinical Laboratory, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, China
Department of Clinical Laboratory, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China
Search for more papers by this authorShiyu Lv
Department of Clinical Laboratory, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China
Search for more papers by this authorWeina Su
Shandong Normal University Campus Hospital, Shandong Normal University, Jinan, China
Search for more papers by this authorCorresponding Author
Wanju Xu
Department of Clinical Laboratory, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China
Correspondence
Wanju Xu, Department of Clinical Laboratory, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, 16766 Jingshi Road, Jinan, Shandong 250014, China.
Email: [email protected]
Search for more papers by this authorHongxiang Xie
Department of Clinical Laboratory, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, China
Department of Clinical Laboratory, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China
Search for more papers by this authorShiyu Lv
Department of Clinical Laboratory, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China
Search for more papers by this authorWeina Su
Shandong Normal University Campus Hospital, Shandong Normal University, Jinan, China
Search for more papers by this authorCorresponding Author
Wanju Xu
Department of Clinical Laboratory, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China
Correspondence
Wanju Xu, Department of Clinical Laboratory, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, 16766 Jingshi Road, Jinan, Shandong 250014, China.
Email: [email protected]
Search for more papers by this authorHongxiang Xie, Shiyu Lv and Weina Su contributed equally to this work.
CONFLICT OF INTEREST
The authors have no significant conflicts of interest to note.
Open Research
DATA AVAILABILITY STATEMENT
The data used to support the findings of this study are available from the corresponding author upon request.
Supporting Information
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| ijlh13556-sup-0001-FigS1.docxWord document, 1.1 MB | Fig S1 |
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REFERENCES
- 1Berentsen S, Ulvestad E, Langholm R, et al. Primary chronic cold agglutinin disease: a population based clinical study of 86 patients. Haematologica. 2006; 91(4): 460-466.
- 2Berentsen S. New insights in the pathogenesis and therapy of cold agglutinin-mediated autoimmune hemolytic anemia. Front Immunol. 2020; 11: 590.
- 3Nakamoto R, Okuyama C, Utsumi T, Yamamoto Y. Splenic marginal zone B-cell lymphoma with splenic infarction in a patient with cold agglutinin disease. Clin Nucl Med. 2019; 44(5): e372-e374.
- 4Lee SC, Youn YS, Rhim JW, Kang JH, Lee KY. Early serologic diagnosis of mycoplasma pneumoniae pneumonia: an observational Study on changes in titers of specific-IgM antibodies and cold agglutinins. Medicine (Baltimore). 2016; 95(19):e3605.
- 5Rim JH, Chang MH, Oh J, Gee HY, Kim JH, Yoo J. Effects of cold agglutinin on the accuracy of complete blood count results and optimal sample pretreatment protocols for eliminating such effects. Ann Lab Med. 2018; 38(4): 371-374.
- 6Nikousefat Z, Javdani M, Hashemnia M, Haratyan A, Jalili A. Cold Agglutinin disease; a laboratory challenge. Iran Red Crescent Med J. 2015; 17(10):e18954.
- 7La Gioia A. Eliminating or minimizing the effects of cold agglutinins on the accuracy of complete blood count results. Ann Lab Med. 2019; 39(5): 499-500.
- 8Godon A, Genevieve F, Marteau-Tessier A, Zandecki M. Automated hematology analysers and spurious counts Part 3. Haemoglobin, red blood cells, cell count and indices, reticulocytes. Ann Biol Clin (Paris). 2012; 70(2): 155-168.
- 9La Gioia A, Fumi M, Fiorini F, et al. Short preheating at 41 degrees C leads to a red blood cells count comparable to that in RET channel of Sysmex analysers in samples showing cold agglutination. J Clin Pathol. 2018; 71(8): 729-734.
- 10Roccaforte V, Sciarini F, Proserpio V, et al. Use of the reticulocyte channel warmed to 41 degrees C of the XN-9000 analyzer in samples with the presence of cold agglutinins. Hematol Transfus Cell Ther. 2020. https://doi.org/10.1016/j.htct.2020.1001.1004
10.1016/j.htct.2020.01.004 Google Scholar
- 11Woodall DW, Brown CJ, Raab SA, et al. Melting of hemoglobin in native solutions as measured by IMS-MS. Anal Chem. 2020; 92(4): 3440-3446.
- 12Lee JH. Neutrophil aggregation on the peripheral blood smear in a patient with cold agglutinin disease. Ann Hematol. 2017; 96(5): 885-886.
- 13Kumar TB, Bhardwaj N. Platelet cold agglutinins and thrombocytopenia: A diagnostic dilemma in the intensive care unit. J Anaesthesiol Clin Pharmacol. 2014; 30(1): 89-90.
- 14Richieri GV, Mel HC. Temperature effects on osmotic fragility, and the erythrocyte membrane. Biochim Biophys Acta. 1985; 813(1): 41-50.
- 15Lu R, Li WW, Katzir A, Raichlin Y, Mizaikoff B, Yu HQ. Fourier transform infrared spectroscopy on external perturbations inducing secondary structure changes of hemoglobin. Analyst. 2016; 141(21): 6061-6067.
- 16Wilen CB, Booth GS, Grossman BJ, Lane WJ, Szklarski PC, Jackups R Jr. Using direct antiglobulin test results to reduce unnecessary cold agglutinin testing. Transfusion. 2017; 57(6): 1480-1484.
