In vitro unexpected effects of polyphosphates observed through activated partial thromboplastin time-based clot waveform analysis
Yuko Ozaki
Clinical Laboratory, Keio University Hospital, Tokyo, Japan
Search for more papers by this authorCorresponding Author
Masatoshi Wakui
Department of Laboratory Medicine, Keio University School of Medicine, Tokyo, Japan
Correspondence
Masatoshi Wakui, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Email: [email protected]
Search for more papers by this authorYuta Fujimori
Office of Clinical Laboratory Technology, Keio University Hospital, Tokyo, Japan
Search for more papers by this authorShusaku Oka
Clinical Laboratory, Keio University Hospital, Tokyo, Japan
Search for more papers by this authorShoko Nakamura
Clinical Laboratory, Keio University Hospital, Tokyo, Japan
Search for more papers by this authorYoshino Kondo
Clinical Laboratory, Keio University Hospital, Tokyo, Japan
Search for more papers by this authorTerumichi Nakagawa
Clinical Laboratory, Keio University Hospital, Tokyo, Japan
Search for more papers by this authorHisako Katagiri
Clinical Laboratory, Keio University Hospital, Tokyo, Japan
Search for more papers by this authorMitsuru Murata
Department of Laboratory Medicine, Keio University School of Medicine, Tokyo, Japan
Search for more papers by this authorYuko Ozaki
Clinical Laboratory, Keio University Hospital, Tokyo, Japan
Search for more papers by this authorCorresponding Author
Masatoshi Wakui
Department of Laboratory Medicine, Keio University School of Medicine, Tokyo, Japan
Correspondence
Masatoshi Wakui, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Email: [email protected]
Search for more papers by this authorYuta Fujimori
Office of Clinical Laboratory Technology, Keio University Hospital, Tokyo, Japan
Search for more papers by this authorShusaku Oka
Clinical Laboratory, Keio University Hospital, Tokyo, Japan
Search for more papers by this authorShoko Nakamura
Clinical Laboratory, Keio University Hospital, Tokyo, Japan
Search for more papers by this authorYoshino Kondo
Clinical Laboratory, Keio University Hospital, Tokyo, Japan
Search for more papers by this authorTerumichi Nakagawa
Clinical Laboratory, Keio University Hospital, Tokyo, Japan
Search for more papers by this authorHisako Katagiri
Clinical Laboratory, Keio University Hospital, Tokyo, Japan
Search for more papers by this authorMitsuru Murata
Department of Laboratory Medicine, Keio University School of Medicine, Tokyo, Japan
Search for more papers by this authorYuko Ozaki (YO) and Masatoshi Wakui (MW) equally contributed to this work and both should be considered first authors.

REFERENCES
- 1Baker CJ, Smith SA, Morrissey JH. Polyphosphate in thrombosis, hemostasis, and inflammation. Res Pract Thromb Haemost. 2018; 3: 18-25.
- 2Puy C, Tucker EI, Wong ZC, et al. Factor XII promotes blood coagulation independent of factor XI in the presence of long-chain polyphosphates. J Thromb Haemost. 2013; 11: 1341-1352.
- 3Mutch NJ, Myles T, Leung LL, et al. Polyphosphate binds with high affinity to exosite II of thrombin. J Thromb Haemost. 2010; 8: 548-555.
- 4Sevenet PO, Depasse F. Clot waveform analysis: where do we stand in 2017? Int J Lab Hematol. 2017; 39: 561-568.
- 5Wakui M, Fujimori Y, Katagiri H, et al. Assessment of in vitro effects of direct thrombin inhibitors and activated factor X inhibitors through clot waveform analysis. J Clin Pathol. 2019; 72: 244-250.
- 6Tan CW, Tan JY, Wong WH, et al. Clinical and laboratory features of hypercoagulability in COVID-19 and other respiratory viral infections amongst predominantly younger adults with few comorbidities. Sci Rep. 2021; 11: 1793.
- 7Salloum-Asfar S, de la Morena-Barrio ME, Esteban J, et al. Assessment of two contact activation reagents for the diagnosis of congenital factor XI deficiency. Thromb Res. 2018; 163: 64-70.
- 8Kumano O, Ieko M, Naito S, et al. APTT reagent with ellagic acid as activator shows adequate lupus anticoagulant sensitivity in comparison to silica-based reagent. J Thromb Haemost. 2012; 10: 2338-2343.
- 9Wijeyewickrema LC, Lameignere E, Hor L, et al. Polyphosphate is a novel cofactor for regulation of complement by a serpin, C1 inhibitor. Blood. 2016; 128: 1766-1776.
- 10Zakharova NV, Artemenko EO, Podoplelova NA, et al. Platelet surface-associated activation and secretion-mediated inhibition of coagulation factor XII. PLoS One. 2015; 10:e0116665.
- 11Henderson MW, Noubouossie DF, Ilich A, et al. Protease: serpin complexes to assess contact system and intrinsic pathway activation. Res Pract Thromb Haemost. 2020; 4: 789-798.
- 12Donovan AJ, Kalkowski J, Smith SA, et al. Size-controlled synthesis of granular polyphosphate nanoparticles at physiologic salt concentrations for blood clotting. Biomacromol. 2014; 15: 3976-3984.