cnm-Positive Streptococcus mutans and diffusion-weighted imaging hyperintensities in acute intracerebral hemorrhage
Yuji Shiga
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Contribution: Formal analysis (equal), Investigation (lead), Writing - original draft (lead)
Search for more papers by this authorCorresponding Author
Shiro Aoki
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Correspondence
Shiro Aoki, Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan.
Email: [email protected]
Contribution: Funding acquisition (equal), Methodology (equal), Project administration (lead), Supervision (equal), Writing - review & editing (lead)
Search for more papers by this authorNaohisa Hosomi
Department of Neurology, Chikamori Hospital, Kochi, Japan
Department of Disease Model, Research Institute of Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan
Contribution: Project administration (lead), Writing - review & editing (equal)
Search for more papers by this authorRyota Nomura
Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka, Japan
Contribution: Investigation (lead), Methodology (lead), Supervision (equal), Writing - review & editing (equal)
Search for more papers by this authorMasahiro Nakamori
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Department of Neurology, Suiseikai Kajikawa Hospital, Hiroshima, Japan
Contribution: Formal analysis (lead), Investigation (lead), Writing - review & editing (equal)
Search for more papers by this authorTomohisa Nezu
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Contribution: Writing - original draft (equal), Writing - review & editing (lead)
Search for more papers by this authorKeisuke Tachiyama
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Department of Neurology, Suiseikai Kajikawa Hospital, Hiroshima, Japan
Contribution: Data curation (equal), Investigation (equal)
Search for more papers by this authorTeppei Kamimura
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Department of Neurology, Suiseikai Kajikawa Hospital, Hiroshima, Japan
Contribution: Data curation (equal), Investigation (equal)
Search for more papers by this authorNaoto Kinoshita
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Department of Neurology, Hiroshima Prefectural Hospital, Hiroshima, Japan
Contribution: Investigation (equal)
Search for more papers by this authorRyo Shimomura
Department of Neurology, Brain Attack Center Ota Memorial Hospital, Fukuyama, Hiroshima, Japan
Contribution: Investigation (supporting)
Search for more papers by this authorYuki Hayashi
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Department of Neurology, Suiseikai Kajikawa Hospital, Hiroshima, Japan
Contribution: Investigation (equal)
Search for more papers by this authorHayato Matsushima
Department of Neurology, Suiseikai Kajikawa Hospital, Hiroshima, Japan
Contribution: Investigation (equal)
Search for more papers by this authorEiji Imamura
Department of Neurology, Suiseikai Kajikawa Hospital, Hiroshima, Japan
Contribution: Data curation (equal), Investigation (equal)
Search for more papers by this authorHiroki Ueno
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Contribution: Data curation (supporting), Investigation (supporting)
Search for more papers by this authorShinichi Wakabayashi
Department of Neurosurgery, Suiseikai Kajikawa Hospital, Hiroshima, Japan
Contribution: Investigation (equal), Writing - review & editing (supporting)
Search for more papers by this authorKazuhiko Nakano
Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka, Japan
Contribution: Funding acquisition (equal), Investigation (lead), Methodology (lead), Project administration (equal), Writing - review & editing (equal)
Search for more papers by this authorTatsuo Kohriyama
Department of Neurology, Brain Attack Center Ota Memorial Hospital, Fukuyama, Hiroshima, Japan
Contribution: Writing - review & editing (supporting)
Search for more papers by this authorHirofumi Maruyama
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Contribution: Supervision (equal), Writing - review & editing (equal)
Search for more papers by this authorYuji Shiga
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Contribution: Formal analysis (equal), Investigation (lead), Writing - original draft (lead)
Search for more papers by this authorCorresponding Author
Shiro Aoki
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Correspondence
Shiro Aoki, Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan.
Email: [email protected]
Contribution: Funding acquisition (equal), Methodology (equal), Project administration (lead), Supervision (equal), Writing - review & editing (lead)
Search for more papers by this authorNaohisa Hosomi
Department of Neurology, Chikamori Hospital, Kochi, Japan
Department of Disease Model, Research Institute of Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan
Contribution: Project administration (lead), Writing - review & editing (equal)
Search for more papers by this authorRyota Nomura
Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka, Japan
Contribution: Investigation (lead), Methodology (lead), Supervision (equal), Writing - review & editing (equal)
Search for more papers by this authorMasahiro Nakamori
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Department of Neurology, Suiseikai Kajikawa Hospital, Hiroshima, Japan
Contribution: Formal analysis (lead), Investigation (lead), Writing - review & editing (equal)
Search for more papers by this authorTomohisa Nezu
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Contribution: Writing - original draft (equal), Writing - review & editing (lead)
Search for more papers by this authorKeisuke Tachiyama
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Department of Neurology, Suiseikai Kajikawa Hospital, Hiroshima, Japan
Contribution: Data curation (equal), Investigation (equal)
Search for more papers by this authorTeppei Kamimura
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Department of Neurology, Suiseikai Kajikawa Hospital, Hiroshima, Japan
Contribution: Data curation (equal), Investigation (equal)
Search for more papers by this authorNaoto Kinoshita
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Department of Neurology, Hiroshima Prefectural Hospital, Hiroshima, Japan
Contribution: Investigation (equal)
Search for more papers by this authorRyo Shimomura
Department of Neurology, Brain Attack Center Ota Memorial Hospital, Fukuyama, Hiroshima, Japan
Contribution: Investigation (supporting)
Search for more papers by this authorYuki Hayashi
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Department of Neurology, Suiseikai Kajikawa Hospital, Hiroshima, Japan
Contribution: Investigation (equal)
Search for more papers by this authorHayato Matsushima
Department of Neurology, Suiseikai Kajikawa Hospital, Hiroshima, Japan
Contribution: Investigation (equal)
Search for more papers by this authorEiji Imamura
Department of Neurology, Suiseikai Kajikawa Hospital, Hiroshima, Japan
Contribution: Data curation (equal), Investigation (equal)
Search for more papers by this authorHiroki Ueno
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Contribution: Data curation (supporting), Investigation (supporting)
Search for more papers by this authorShinichi Wakabayashi
Department of Neurosurgery, Suiseikai Kajikawa Hospital, Hiroshima, Japan
Contribution: Investigation (equal), Writing - review & editing (supporting)
Search for more papers by this authorKazuhiko Nakano
Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka, Japan
Contribution: Funding acquisition (equal), Investigation (lead), Methodology (lead), Project administration (equal), Writing - review & editing (equal)
Search for more papers by this authorTatsuo Kohriyama
Department of Neurology, Brain Attack Center Ota Memorial Hospital, Fukuyama, Hiroshima, Japan
Contribution: Writing - review & editing (supporting)
Search for more papers by this authorHirofumi Maruyama
Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
Contribution: Supervision (equal), Writing - review & editing (equal)
Search for more papers by this authorAbstract
Background
Strains of Streptococcus mutans expressing the cell surface collagen-binding protein, Cnm, encoded by cnm (cnm-positive S. mutans), are associated with hypertensive intracerebral hemorrhage (ICH) and the occurrence of cerebral microbleeds (CMBs). Small diffusion-weighted imaging (DWI) hyperintensities in patients with acute ICH are also associated with CMBs. However, the association between cnm-positive S. mutans and DWI hyperintensities is unclear. Hence, this study aimed to investigate the association between cnm-positive S. mutans and DWI hyperintensities in patients with acute ICH.
Methods
Patients with acute ICH were prospectively registered at three hospitals. Dental plaque specimens were collected within 4 days after admission, and cnm-positive S. mutans was detected using the polymerase chain reaction. Magnetic resonance imaging at 14 ± 5 days after admission was used to evaluate DWI hyperintensities and CMBs.
Results
A total of 197 patients were enrolled in this study. cnm-positive S. mutans was detected in 30 patients (15.2%), and DWI hyperintensities were observed in 56 patients (28.4%). Patients with cnm-positive S. mutans had a higher frequency of DWI hyperintensities (50.0% vs 24.6%; p = 0.008) and a higher number of CMBs (5.5 vs 1.5; p < 0.001) than those without cnm-positive S. mutans. Multivariable logistic analysis revealed that the presence of cnm-positive S. mutans was independently associated with DWI hyperintensities (OR 2.38; 95% CI 1.01–5.61; p = 0.047) after adjusting for several confounding factors.
Conclusion
This study found an association between the presence of cnm-positive S. mutans and DWI hyperintensities in patients with acute ICH.
CONFLICTS OF INTEREST
Dr. Maruyama received Grants-in-Aid for Scientific Research (20 K07887) and research support from Eisai, Pfizer, Takeda Pharmaceutical, Otsuka Pharmaceutical, Nihon Pharmaceutical, Shionogi, Teijin Pharma, Fuji Film, Sumitomo Dainippon Pharma, Nihon Medi-Physics, Bayer, MSD, Daiichi Sankyo, Kyowa Kirin, Sanofi, Novartis, Kowa Pharmaceutical, Astellas Pharma, Tsumura, Japan Blood Products Organization, and Mitsubishi Tanabe Pharma. All other authors declare that they have no conflicts of interest.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author on reasonable request.
Supporting Information
| Filename | Description |
|---|---|
| ene14725-sup-0001-TableS1.pdfPDF document, 98.8 KB | Table S1 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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