Soft tissue histomorphology at implants with a transmucosal modified surface. A study in minipigs
Corresponding Author
Antonio Liñares
Periodontology Unit, School of Medicine and Dentistry, University of Santiago de Compostela, Santiago de Compostela, Spain
Corresponding author:
Dr. Antonio Liñares
Periodontology Unit
School of Medicine and Dentistry
University of Santiago de Compostela
Rúa San Francisco
s/n 15782 Santiago de Compostela
Spain
Tel.: +34 62 649 2454
Fax: +34 981 571826
e-mail: [email protected]
Search for more papers by this authorFernando Muñoz
Department of Veterinary Clinical Sciences, University of Santiago de Compostela, Lugo, Spain
Search for more papers by this authorMaría Permuy
Department of Veterinary Clinical Sciences, University of Santiago de Compostela, Lugo, Spain
Search for more papers by this authorMichel Dard
Department of Periodontology and Implant Dentistry, College of Dentistry, New York University, New York, NY, USA
Search for more papers by this authorJuan Blanco
Periodontology Unit, School of Medicine and Dentistry, University of Santiago de Compostela, Santiago de Compostela, Spain
Search for more papers by this authorCorresponding Author
Antonio Liñares
Periodontology Unit, School of Medicine and Dentistry, University of Santiago de Compostela, Santiago de Compostela, Spain
Corresponding author:
Dr. Antonio Liñares
Periodontology Unit
School of Medicine and Dentistry
University of Santiago de Compostela
Rúa San Francisco
s/n 15782 Santiago de Compostela
Spain
Tel.: +34 62 649 2454
Fax: +34 981 571826
e-mail: [email protected]
Search for more papers by this authorFernando Muñoz
Department of Veterinary Clinical Sciences, University of Santiago de Compostela, Lugo, Spain
Search for more papers by this authorMaría Permuy
Department of Veterinary Clinical Sciences, University of Santiago de Compostela, Lugo, Spain
Search for more papers by this authorMichel Dard
Department of Periodontology and Implant Dentistry, College of Dentistry, New York University, New York, NY, USA
Search for more papers by this authorJuan Blanco
Periodontology Unit, School of Medicine and Dentistry, University of Santiago de Compostela, Santiago de Compostela, Spain
Search for more papers by this authorAbstract
Objectives
To investigate soft tissue histomorphology and quality around implants with a modified transgingival collar surface comparatively to a machined.
Material and methods
Twenty-seven Straumann Standard Tissue Level implants belonging to the following groups (nine of each group): Ti modSLA with machined collar (Ti-M), Ti modSLA with machined, acid-etched surface collar (Ti-modMA), and TiZr modSLA with machined, acid-etched surface collar (TiZr-modMA) were placed in the mandible of six minipigs. After 8 weeks of healing, buccal sections were obtained and processed for histological evaluation.
Results
Histometric soft tissue outcomes were similar for the three types of implants. The percentage of connective tissue attached to implant surface and its length was longer at TiZr-modMA with respect to Ti-M implants. The number of inflammatory cells was slightly higher at the TiZr-modMA with respect to Ti-M implant. The percentage of area occupied by perpendicular collagen fibers was slightly higher for the modified surfaces in comparison with the machined.
Conclusions
Modified implant collar surfaces at Ti and TiZr implants showed a soft tissue interface similar to machined. A tendency of increasing number of perpendicular collagen fibers and improved connective tissue contact was found at the modified implant surfaces.
References
- Abrahamsson, I., Berglundh, T., Moon, I.S. & Lindhe, J. (1999) Periimplant tissues at submerged titanium implants. Journal of Clinical Periodontology 26: 600–607.
- Abrahamsson, I., Berglundh, T., Wennström, J. & Lindhe, J. (1996) The peri-implant hard and soft tissues at different implant systems. A comparative study in the dog. Clinical Oral Implants Research 7: 212–219.
- Atieh, M.A., Alsabeeha, N.H., Faggion, C.M., Jr & Duncan, W.J. (2013) The frequency of peri-implant diseases: a systematic review and meta-analysis. Journal of Periodontology 11: 1586–1598.
- Berglundh, T., Lindhe, J., Ericsson, I., Marinello, C.P., Liljenberg, B. & Thomsen, P. (1991) The soft tissue barrier at implants and teeth. Clinical Oral Implants Research 2: 81–90.
- Brunner, E. (2010) SAS standard procedures for the analysis of non-parametric data. (In German). http://saswiki.org/images/e/e3/5.KSFE-2001-brunner-Einsatz-von-SAS-Modulen-f%C3%BCr-die-nichtparametrische-Auswertung-von-longitudinalen-Daten.pdf
- Brunner, E. & Langer, F. (1999) Non-Parametric Analysis of Longitudinal Data. (In German). München: Oldenburgverlag.
- Buser, D., Weber, H.P., Donath, K., Fiorellini, J.P., Paquette, D.W. & Williams, R.C. (1992) Soft tissue reactions to non-submerged unloaded titanium implants in beagle dogs. Journal of Periodontology 63: 225–235.
- Cochran, D.L., Hermann, J.S., Schenk, R.K., Higginbottom, F.L. & Buser, D. (1997) Biologic width around titanium implants. A histometric analysis of the implanto-gingival junction around unloaded and loaded nonsubmerged implants in the canine mandible. Journal of Periodontology 68: 186–198.
- Dard, M. (2012) Methods and interpretation of performance studies for dental implants. In: J. Boutrand, ed. Biocompatibility and Performance of Medical Devices, 308–344. Cambridge, UK: Woodhead Publishing Ltd.
- Delgado-Ruiz, R.A., Calvo-Guirado, J.L., Abboud, M., Ramirez-Fernandez, M.P., Mate-Sanchez, J.E., Negri, B., Gomez-Moreno, G. & Markovic, A. (2013) Connective tissue characteristics around healing abutments of different geometries: new methodological technique under circularly polarized light. Clinical Implant Dentistry and Related Research doi: 10.1111/cid.12161.
10.1111/cid.12161 Google Scholar
- Donath, K. & Breuner, G. (1982) A method for the study of undecalcified bones and teeth with attached soft tissues. The Säge-Schliff (sawing and grinding) technique. Journal of Oral Pathology 11: 318–326.
- Gottlow, J., Dard, M., Kjellson, F., Obrecht, M. & Sennerby, L. (2012) Evaluation of a new titanium-zirconium dental implant. A biomechanical and histological comparative study in the mini-pig. Clinical Implant Dentistry and Related Research 14: 538–545.
- Hämmerle, C.H., Brägger, U., Bürgin, W. & Lang, N.P. (1996) The effect of subcrestal placement of the polished surface of ITI implants on marginal soft and hard tissues. Clinical Oral Implants Research 7: 111–119.
- Heitz-Mayfield, L.J. (2008) Peri-implant diseases: diagnosis and risk indicators. Journal of Clinical Periodontology 35: 292–304.
- Hermann, J.S., Buser, D., Schenk, R.K., Schoolfield, J.D. & Cochran, D.L. (2001) Biologic width around one- and two-piece titanium implants. Clinical Oral Implants Research 12: 559–571.
- Jung, R.E., Zembic, A., Pjetursson, B.E., Zwahlen, M. & Thoma, D.S. (2012) Systematic review of the survival rate and the incidence of biological, technical and esthetic complications of single crowns on implants reported in longitudinal studies with a mean follow-up of 5 years. Clinical Oral Implants Research 23(Suppl. 6): 2–21.
- Kilkenny, C., Browne, W., Cuthill, I.C., Emerson, M. & Altman, D.G. (2011) Animal research: reporting in vivo experiments – the ARRIVE guidelines. Journal of Cerebral Blood Flow & Metabolism 31: 991–993.
- Laczkó, J. & Levai, G. (1975) A simple differential staining method for semi-thin sections of ossifying cartilage and bone tissues embedded in epoxy resin. Mikroskopie 31: 1–4.
- Liñares, A., Domken, O., Dard, M. & Blanco, J. (2013) Peri-implant soft tissues around implants with a modified neck surface. Part 1. Clinical and histometric outcomes: a pilot study in minipigs. Journal of Clinical Periodontology 40: 412–420.
- Lindhe, J. & Berglundh, T. (1998) The interface between the mucosa and the implant. Periodontology 2000 17: 47–54.
- Listgarten, M.A., Buser, D., Steinemann, S.G., Donath, K., Lang, N.P. & Weber, H.P. (1992) Light and transmission electron microscopy of the intact interfaces between non-submerged titanium-coated epoxy resin implants and bone or gingiva. Journal of Dental Research 71: 364–371.
- Mareque, S., Liñares, A., Pérez, J., Muñoz, F., Ramos, I. & Blanco, J. (2013) Impact of immediate loading on early soft tissue healing at two-piece implants placed in fresh extraction sockets: an experimental study in the beagle dog. Clinical Oral Implants Research doi:10.1111/clr.12187.
- Mombelli, A., Müller, N. & Cionca, N. (2012) The epidemiology of peri-implantitis. Clinical Oral Implants Research 23(Suppl. 6): 67–76.
- Nevins, M., Nevins, M.L., Camelo, M., Boyesen, J.L. & Kim, D.M. (2008) Human histologic evidence of a connective tissue attachment to a dental implant. International Journal of Periodontics and Restorative Dentistry 28: 111–1121.
- Pjetursson, B.E., Thoma, D., Jung, R., Zwahlen, M. & Zembic, A. (2012) A systematic review of the survival and complication rates of implant-supported fixed dental prostheses (FDPs) after a mean observation period of at least 5 years. Clinical Oral Implants Research 23(Suppl. 6): 22–38.
- Rodríguez, X., Vela, X., Calvo-Guirado, J.L., Nart, J. & Stappert, C.F. (2012) Effect of platform switching on collagen fiber orientation and bone resorption around dental implants: a preliminary histologic animal study. The International Journal of Oral & Maxillofacial Implants 27: 1116–1122.
- Romanos, G.E., Schröter-Kermani, C., Weingart, D. & Strub, J.R. (1995) Health human periodontal versus peri-implant gingival tissues: an immunohistochemical differentiation of the extracellular matrix. The International Journal of Oral & Maxillofacial Implants 10: 750–758.
- Romeo, E. & Storelli, S.. (2012) Systematic review of the survival rate and the biological, technical and esthetic complications of fixed dental prostheses with cantilevers on implants reported in longitudinal studies with a mean of 5 years follow-up. Clinical Oral Implants Research 23(Suppl. 6): 39–49.
- Rompen, E., Domken, O., Degidi, M., Pontes, A.E.F. & Piattelli, A. (2006) The effect of material characteristics, of surface topography and of implant components and connections on soft tissue integration: a literature review. Clinical Oral Implants Research 17: 55–67.
- Schwarz, F., Ferrari, D., Herten, M., Mihatovic, I., Wieland, M., Sager, M. & Becker, J. (2007b) Effects of surface hydrophilicity and microtopography on early stages of soft and hard tissue integration at non-submerged titanium implants: an immunohistochemical study in dogs. Journal of Periodontology 78: 2171–2184.
- Schwarz, F., Herten, M., Sager, M., Wieland, M., Dard, M. & Becker, J. (2007a) Histological and immunohistochemical analysis of initial and early subepithelial connective tissue attachment at chemically modified and conventional SLA titanium implants. A pilot study in dogs. Clinical Oral Investigations 11: 245–255.
- Schwarz, F., Mihatovic, I., Becker, J., Bormann, K.H., Keeve, P. & Friedmann, A. (2013) Histological evaluation of different abutments in the posterior maxilla and mandible. An experimental study in humans. Journal of Clinical Periodontology 40: 266–286.
- Schwarz, F., Mihatovic, I., Ferrari, D., Wieland, M. & Becker, J. (2010) Influence of frequent clinical probing during the healing phase on healthy peri-implant soft tissue formed at different titanium implant surfaces: a histomorphometrical study in dogs. Journal of Clinical Periodontology 37: 551–562.
- Tetè, S., Mastrangelo, F., Bianchi, A., Zizzari, V. & Scarano, A. (2009) Collagen fiber orientation around machined titanium and zirconia dental implant necks: an animal study. International Journal of Oral & Maxillofacial Implants 24: 52–58.
- Thoma, D.S., Jones, A.A., Dard, M., Grize, L., Obrecht, M. & Cochran, D.L. (2011) Tissue integration of a new titanium-zirconium dental implant: a comparative histologic and radiographic study in the canine. Journal of Periodontology 82: 1453–1461.
- Tonetti, M.S., Imboden, M., Gerber, L. & Lang, N.P. (1995) Compartmentalization of inflammatory cell phenotypes in normal gingiva and peri-implant keratinized mucosa. Journal of Clinical Periodontology 22: 735–742.
- Traini, T., Neugebauer, J., Thams, U., Zöller, J.E., Caputi, S. & Piatelli, A. (2008) Peri-implant bone organization under immediate loading conditions: collagen fiber orientation and mineral density analyses in the minipig model. Clinical Implant Dentistry and Related Research 11: 41–51.
- de Waal, Y.C.M., vanWinkelhoff, A.J., Meijer, H.J.A., Raghoebar, G.M. & Winkel, E.G. (2013) Differences in peri-implant conditions between fully and partially edentulous subjects: a systematic review. Journal of Clinical Periodontology 40: 266–286.
- Weber, H.P., Buser, D., Donath, K., Fiorellini, J.P., Doppalapudi, V., Paquette, D.W. & Williams, R.C. (1996) Comparison of healed tissues adjacent to submerged and non submerged unloaded titanium dental implants. Clinical Oral Implants Research 7: 11–19.
- Weiner, S., Simon, J., Ehrenberg, D.S., Zweig, B. & Ricci, J.L. (2008) The effects of laser microtextured collars upon crestal bone levels of dental implants. Implant Dentistry 17: 217–228.
- Werner, S., Huck, O., Frisch, B., Vautier, D., Elkaim, R., Voegel, J.C., Brunel, G. & Tenenbaum, H. (2009) The effect of microstructured surfaces and laminin-derived peptide coatings on soft tissue interactions with titanium dental implants. Biomaterials 30: 2291–2301.
- Zhao, B.H., Han, H., Feng, H.L., Bai, W., Cui, F.Z. & Lee, I.S. (2013) Histomorphometrical and clinical study of connective tissue around titanium dental implants with porous surfaces in a canine model. Journal of Biomaterials Applications 27: 685–693.
- Zitzmann, N.U. & Berglundh, T. (2008) Definition and prevalence of peri-implant diseases. Journal of Clinical Periodontology 35: 286–291.
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