M2 polarization of monocytes in ankylosing spondylitis and relationship with inflammation and structural damage
Jinzhu Zhao
Department of Orthopedics, No. 401 Hospital of PLA, Qingdao, Shandong, China
Search for more papers by this authorWei Yuan
Department of Outpatient Clinic, No. 1 Sanatorium of Qingdao of PLA, Qingdao, Shandong, China
Search for more papers by this authorChunsheng Tao
Department of Orthopedics, No. 401 Hospital of PLA, Qingdao, Shandong, China
Search for more papers by this authorPeifeng Sun
Department of Orthopedics, No. 401 Hospital of PLA, Qingdao, Shandong, China
Search for more papers by this authorCorresponding Author
Zaixing Yang
Department of Laboratory Medicine, Taizhou First People's Hospital, Huangyan Hospital of Wenzhou Medical University, Taizhou, Zhejiang, China
Zaixing Yang, Department of Laboratory Medicine, Taizhou First People's Hospital, Huangyan Hospital of Wenzhou Medical University, No. 218 Hengjie Road, Taizhou, Zhejiang 318020, China. e-mail: [email protected]
and
Weidong Xu, Department of Orthopedics, Changhai Hospital, Second Military Medical University, No. 168 Changhai Road, Shanghai 200433, China. e-mail: [email protected]
Search for more papers by this authorCorresponding Author
Weidong Xu
Department of Orthopedics, Changhai Hospital, Second Military Medical University, Shanghai, China
Zaixing Yang, Department of Laboratory Medicine, Taizhou First People's Hospital, Huangyan Hospital of Wenzhou Medical University, No. 218 Hengjie Road, Taizhou, Zhejiang 318020, China. e-mail: [email protected]
and
Weidong Xu, Department of Orthopedics, Changhai Hospital, Second Military Medical University, No. 168 Changhai Road, Shanghai 200433, China. e-mail: [email protected]
Search for more papers by this authorJinzhu Zhao
Department of Orthopedics, No. 401 Hospital of PLA, Qingdao, Shandong, China
Search for more papers by this authorWei Yuan
Department of Outpatient Clinic, No. 1 Sanatorium of Qingdao of PLA, Qingdao, Shandong, China
Search for more papers by this authorChunsheng Tao
Department of Orthopedics, No. 401 Hospital of PLA, Qingdao, Shandong, China
Search for more papers by this authorPeifeng Sun
Department of Orthopedics, No. 401 Hospital of PLA, Qingdao, Shandong, China
Search for more papers by this authorCorresponding Author
Zaixing Yang
Department of Laboratory Medicine, Taizhou First People's Hospital, Huangyan Hospital of Wenzhou Medical University, Taizhou, Zhejiang, China
Zaixing Yang, Department of Laboratory Medicine, Taizhou First People's Hospital, Huangyan Hospital of Wenzhou Medical University, No. 218 Hengjie Road, Taizhou, Zhejiang 318020, China. e-mail: [email protected]
and
Weidong Xu, Department of Orthopedics, Changhai Hospital, Second Military Medical University, No. 168 Changhai Road, Shanghai 200433, China. e-mail: [email protected]
Search for more papers by this authorCorresponding Author
Weidong Xu
Department of Orthopedics, Changhai Hospital, Second Military Medical University, Shanghai, China
Zaixing Yang, Department of Laboratory Medicine, Taizhou First People's Hospital, Huangyan Hospital of Wenzhou Medical University, No. 218 Hengjie Road, Taizhou, Zhejiang 318020, China. e-mail: [email protected]
and
Weidong Xu, Department of Orthopedics, Changhai Hospital, Second Military Medical University, No. 168 Changhai Road, Shanghai 200433, China. e-mail: [email protected]
Search for more papers by this authorAbstract
The aim of this study was to evaluate the polarization of peripheral blood monocytes in the patients with ankylosing spondylitis (AS) and to determine the correlations between monocyte polarization and inflammation and structural damage. A total of 120 AS patients, 50 rheumatoid arthritis (RA) patients and 100 healthy controls were enrolled in the study. M1 (CD68+CD192+) and M2 (CX3CR1+CD163+) monocytes were characterized by flow cytometry. Demographic, clinical, radiographic and laboratory data were collected and analyzed. A large increase in M2 (CX3CR1+CD163+) monocytes was observed in AS, and M2/M1 ratio was 7.18 ± 6.12, 2.54 ± 3.14 and 35.61 ± 20.04 in control, RA and AS, respectively. The M2/M1 ratio correlated with modified Stoke Ankylosing Spondylitis Spine Score (mSASSS) (r = 0.565; p < 0.001), ESR (r = −0.321; p < 0.001, CRP (r = −0.265; p < 0.001) and Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) (r = −0.201; p = 0.028). Anti-TNF-α therapy induced a significant reduction in the percentage of M1 monocyte, ESR, CRP and BASDAI in AS patients. The present results demonstrated that M2 type polarized monocytes are predominant in the peripheral blood in AS and the M2/M1 ratio is correlated with structural damage (mSASSS), inflammatory biomarkers (ESR and CRP) and BASDAI.
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