Effects of aescin on testicular repairment in rats with experimentally induced varicocele
R. H. Tian
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorM. Ma
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorY. Zhu
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorS. Yang
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorZ. Q. Wang
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorZ. S. Zhang
Department of Ultrasonography, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorC. F. Wan
Department of Ultrasonography, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorP. Li
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorY. F. Liu
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorJ. L. Wang
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorY. Liu
Clinical Stem Cell Centre, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorH. Yang
Clinical Stem Cell Centre, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorZ. Z. Zhang
Clinical Stem Cell Centre, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorL. H. Liu
Clinical Stem Cell Centre, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorY. H. Gong
Clinical Stem Cell Centre, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorF. H. Li
Department of Ultrasonography, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorH. L. Hu
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorZ. P. He
Clinical Stem Cell Centre, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorY. R. Huang
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorCorresponding Author
Z. Li
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Correspondence
Dr Zheng Li, Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 845 Lingshan Road, Shanghai 200135, China.
Tel.: +86 21 20284582;
Fax: +86 21 20284583;
E-mail: [email protected]
Search for more papers by this authorR. H. Tian
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorM. Ma
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorY. Zhu
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorS. Yang
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorZ. Q. Wang
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorZ. S. Zhang
Department of Ultrasonography, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorC. F. Wan
Department of Ultrasonography, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorP. Li
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorY. F. Liu
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorJ. L. Wang
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorY. Liu
Clinical Stem Cell Centre, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorH. Yang
Clinical Stem Cell Centre, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorZ. Z. Zhang
Clinical Stem Cell Centre, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorL. H. Liu
Clinical Stem Cell Centre, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorY. H. Gong
Clinical Stem Cell Centre, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorF. H. Li
Department of Ultrasonography, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorH. L. Hu
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorZ. P. He
Clinical Stem Cell Centre, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorY. R. Huang
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Search for more papers by this authorCorresponding Author
Z. Li
Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
Correspondence
Dr Zheng Li, Department of Urology, Shanghai Human Sperm Bank, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 845 Lingshan Road, Shanghai 200135, China.
Tel.: +86 21 20284582;
Fax: +86 21 20284583;
E-mail: [email protected]
Search for more papers by this authorSummary
This study was conducted to investigate the effects of aescin treatment in a rodent model treated with an experimentally induced varicocele. Experimental varicocele was induced by partial ligation of the left renal vein of rats. Aescin administration was performed daily for 4 weeks after the varicocele induction. Seven weeks later, a contrast-enhanced ultrasound was performed of the rats' testis to assess testicular blood flow. The animals were sacrificed, and H&E staining was then used to evaluate testicular pathological changes and polymorphonuclear leucocytes density. Cauda epididymal sperm counts and motility were evaluated. Blood was collected for the measurement of follicle-stimulating hormone, luteinising hormone and testosterone. Contrast-enhanced ultrasound showed that there were significant decreases in testicular blood flow in the aescin-treated groups compared with those in control varicocele group. Testicular oedema was detected in those rats treated with a varicocele but without aescin, while no oedema was found in the experimental group. H&E staining showed dysfunctional spermatogenesis in both cohorts; however, polymorphonuclear leucocytes density was significantly reduced in aescin-treated groups. There was an increase in sperm counts of the aescin-treated groups. Our study demonstrated that aescin could exert therapeutical effects on reversal of testicular lesions in varicocele rats.
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