Volume 68, Issue 7 pp. 862-869

Original Article

Resveratrol prevents development of eosinophilic rhinosinusitis with nasal polyps in a mouse model

S.-W. Kim,

S.-W. Kim

Department of Otorhinolaryngology, Gyeongsang National University Hospital, Jinju, South Korea

Institute of Health Sciences, Gyeongsang National University, Jinju, South Korea

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D. W. Kim,

D. W. Kim

Department of Otorhinolaryngology, Seoul National University College of Medicine, Seoul, South Korea

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R. Khalmuratova,

R. Khalmuratova

Department of Otorhinolaryngology, Gyeongsang National University Hospital, Jinju, South Korea

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J. H. Kim,

J. H. Kim

Institute of Health Sciences, Gyeongsang National University, Jinju, South Korea

Clinical Research Institute, Gyeongsang National University Hospital, Jinju, South Korea

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M. H. Jung,

M. H. Jung

Clinical Research Institute, Gyeongsang National University Hospital, Jinju, South Korea

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D.-Y. Chang,

D.-Y. Chang

Laboratory of Immunology and Infectious Diseases, Graduate School of Medical Science and Engineering, KAIST, Daejeon, South Korea

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E.-C. Shin,

E.-C. Shin

Laboratory of Immunology and Infectious Diseases, Graduate School of Medical Science and Engineering, KAIST, Daejeon, South Korea

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H.-K. Lee,

H.-K. Lee

Department of Otorhinolaryngology, Gyeongsang National University Hospital, Jinju, South Korea

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H.-W. Shin,

H.-W. Shin

Department of Pharmacology and Biomedical Science, Ischemic/Hypoxic Disease Institute, Seoul National University College of Medicine, Seoul, South Korea

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C.-S. Rhee,

C.-S. Rhee

Department of Otorhinolaryngology, Seoul National University College of Medicine, Seoul, South Korea

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S.-Y. Jeon,

S.-Y. Jeon

Department of Otorhinolaryngology, Gyeongsang National University Hospital, Jinju, South Korea

Institute of Health Sciences, Gyeongsang National University, Jinju, South Korea

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Y.-G. Min,

Corresponding Author

Y.-G. Min

Department of Otolaryngology-Head and Neck Surgery, National Medical Center, Seoul, South Korea

Correspondence

Yang-Gi Min, Department of Otolaryngology-Head and Neck Surgery, National Medical Center, 18-79 Euljiro 6-ga, Jung-gu, Seoul 100-799, South Korea.

Tel.: +82-10-5409-4892

Fax: +82-2-2276-0534

E1-mail: [email protected]

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S.-W. Kim,

S.-W. Kim

Department of Otorhinolaryngology, Gyeongsang National University Hospital, Jinju, South Korea

Institute of Health Sciences, Gyeongsang National University, Jinju, South Korea

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D. W. Kim,

D. W. Kim

Department of Otorhinolaryngology, Seoul National University College of Medicine, Seoul, South Korea

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R. Khalmuratova,

R. Khalmuratova

Department of Otorhinolaryngology, Gyeongsang National University Hospital, Jinju, South Korea

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J. H. Kim,

J. H. Kim

Institute of Health Sciences, Gyeongsang National University, Jinju, South Korea

Clinical Research Institute, Gyeongsang National University Hospital, Jinju, South Korea

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M. H. Jung,

M. H. Jung

Clinical Research Institute, Gyeongsang National University Hospital, Jinju, South Korea

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D.-Y. Chang,

D.-Y. Chang

Laboratory of Immunology and Infectious Diseases, Graduate School of Medical Science and Engineering, KAIST, Daejeon, South Korea

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E.-C. Shin,

E.-C. Shin

Laboratory of Immunology and Infectious Diseases, Graduate School of Medical Science and Engineering, KAIST, Daejeon, South Korea

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H.-K. Lee,

H.-K. Lee

Department of Otorhinolaryngology, Gyeongsang National University Hospital, Jinju, South Korea

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H.-W. Shin,

H.-W. Shin

Department of Pharmacology and Biomedical Science, Ischemic/Hypoxic Disease Institute, Seoul National University College of Medicine, Seoul, South Korea

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C.-S. Rhee,

C.-S. Rhee

Department of Otorhinolaryngology, Seoul National University College of Medicine, Seoul, South Korea

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S.-Y. Jeon,

S.-Y. Jeon

Department of Otorhinolaryngology, Gyeongsang National University Hospital, Jinju, South Korea

Institute of Health Sciences, Gyeongsang National University, Jinju, South Korea

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Y.-G. Min,

Corresponding Author

Y.-G. Min

Department of Otolaryngology-Head and Neck Surgery, National Medical Center, Seoul, South Korea

Correspondence

Yang-Gi Min, Department of Otolaryngology-Head and Neck Surgery, National Medical Center, 18-79 Euljiro 6-ga, Jung-gu, Seoul 100-799, South Korea.

Tel.: +82-10-5409-4892

Fax: +82-2-2276-0534

E1-mail: [email protected]

Search for more papers by this author

First published: 10 June 2013

https://doi.org/10.1111/all.12132

Citations: 44

Edited by: Wytske Fokkens

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Abstract

Background

Since the recent establishment of a murine model of eosinophilic chronic rhinosinusitis with nasal polyps (CRSwNP), both the development of new drugs for treatment or prevention of eosinophilic CRSwNP and elucidation of their pathogenesis have been feasible. We investigated the therapeutic effects of resveratrol on CRSwNP and its mechanism of action using a murine model.

Methods

After induction of eosinophilic CRSwNP, the therapeutic effects of resveratrol were tested and compared with those of triamcinolone acetonide. Histopathologic changes were evaluated using hematoxylin and eosin for overall inflammation, Sirius red for eosinophils, and Masson's trichrome stain for collagen. The expression levels of the interleukin (IL)-4, IL-5, prostaglandin D synthase, and leukotriene C₄ synthase genes were assessed by quantitative real-time PCR. Cyclooxygense-2 and 5-lipoxygense levels were evaluated by immunohistochemical staining and Western blot analysis.

Results

The degree of eosinophilic infiltration and subepithelial fibrosis was significantly decreased by administration of high-dose resveratrol, the potency of which was similar to that of triamcinolone acetonide. The expression levels of the IL-4, IL-5, prostaglandin D synthase, and leukotriene C₄ synthase genes were significantly decreased by administration of low- or high-dose resveratrol. The production of 5-lipoxygenase was strongly inhibited by high-dose resveratrol.

Conclusions

Resveratrol may be useful for the prevention of eosinophilic CRSwNP. A key mechanism of its action is believed to be its anti-inflammatory effect, particularly on eosinophils, by inhibiting the lipoxygenase pathway.

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Volume68, Issue7

July 2013

Pages 862-869

Resveratrol prevents development of eosinophilic rhinosinusitis with nasal polyps in a mouse model

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Resveratrol prevents development of eosinophilic rhinosinusitis with nasal polyps in a mouse model

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