Aberrant expressions of endometrial Id3 and CTLA-4 are associated with unexplained repeated implantation failure and recurrent miscarriage
Jin-Li Ding
Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China
These authors contributed equally to this work.Search for more papers by this authorLiang-Hui Diao
Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China
These authors contributed equally to this work.Search for more papers by this authorTai-Lang Yin
Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China
Search for more papers by this authorChun-Yu Huang
Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China
Search for more papers by this authorBiao Yin
Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China
Search for more papers by this authorCong Chen
Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China
Search for more papers by this authorYi Zhang
Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China
Search for more papers by this authorJie Li
Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China
Search for more papers by this authorYan-Xiang Cheng
Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China
Search for more papers by this authorCorresponding Author
Yong Zeng
Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China
Correspondence
Yong Zeng, Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China.
Email: [email protected]
Jing Yang, Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Jing Yang
Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China
Correspondence
Yong Zeng, Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China.
Email: [email protected]
Jing Yang, Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China.
Email: [email protected]
Search for more papers by this authorJin-Li Ding
Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China
These authors contributed equally to this work.Search for more papers by this authorLiang-Hui Diao
Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China
These authors contributed equally to this work.Search for more papers by this authorTai-Lang Yin
Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China
Search for more papers by this authorChun-Yu Huang
Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China
Search for more papers by this authorBiao Yin
Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China
Search for more papers by this authorCong Chen
Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China
Search for more papers by this authorYi Zhang
Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China
Search for more papers by this authorJie Li
Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China
Search for more papers by this authorYan-Xiang Cheng
Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China
Search for more papers by this authorCorresponding Author
Yong Zeng
Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China
Correspondence
Yong Zeng, Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China.
Email: [email protected]
Jing Yang, Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Jing Yang
Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China
Correspondence
Yong Zeng, Shenzhen Key Laboratory for Reproductive Immunology of Peri-implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China.
Email: [email protected]
Jing Yang, Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, China.
Email: [email protected]
Search for more papers by this authorAbstract
Inhibitor of DNA-binding protein 3 (Id3) is required for tumor angiogenesis and regulatory T-cell generation. However, the involvement of Id3 in unexplained repeated implantation failure (RIF) and recurrent miscarriage (RM) remains poorly understood. Immunohistochemistry was used to identify Id3, CD34, CTLA-4, and FOXP3 in the endometrium taken from the women with RIF (n=16), RM (n=16) and matched controls (n=8). The images were acquired and analyzed by the Vectra® automated quantitative pathology imaging system. Percentage of Id3+ cells was significantly higher in the endometrium of women with RIF and RM compared with controls. The numbers of Id3+ and CD34+ vessels in the endometrium were positively correlated in control but not in RIF or RM. Percentages of CTLA-4+ cells, but not FOXP3+ cells, were significantly increased in the endometrium of RIF and RM women than those in controls. We found aberrant expressions of endometrial Id3 and CTLA-4 in peri-implantation endometrium of women with RIF and RM, suggesting the negative roles of these angiogenesis and immune tolerance markers involving in regulating endometrium receptivity.
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