American Journal of Reproductive Immunology

Short Communication

Is HLA-E a possible genetic marker relevant for natural conception?

Georgia Fernanda Gelmini

Laboratório de Imunogenética e Histocompatibilidade do Departamento de Genética da Universidade Federal do Paraná (UFPR), Curitiba (PR), Brasil

Search for more papers by this author

Cynthia Hernandes Costa,

Cynthia Hernandes Costa

Laboratório de Imunogenética e Histocompatibilidade do Departamento de Genética da Universidade Federal do Paraná (UFPR), Curitiba (PR), Brasil

Search for more papers by this author

Fabiola da Silva Nardi,

Fabiola da Silva Nardi

Laboratório de Imunogenética e Histocompatibilidade do Departamento de Genética da Universidade Federal do Paraná (UFPR), Curitiba (PR), Brasil

Search for more papers by this author

Pryscilla Fanini Wowk,

Pryscilla Fanini Wowk

Laboratório de Imunogenética e Histocompatibilidade do Departamento de Genética da Universidade Federal do Paraná (UFPR), Curitiba (PR), Brasil

Instituto Carlos Chagas, ICC-Fiocruz-PR, Curitiba (PR), Brasil

Search for more papers by this author

Sibelle Botogosque Mattar,

Sibelle Botogosque Mattar

Laboratório de Imunogenética e Histocompatibilidade do Departamento de Genética da Universidade Federal do Paraná (UFPR), Curitiba (PR), Brasil

Instituto Carlos Chagas, ICC-Fiocruz-PR, Curitiba (PR), Brasil

Search for more papers by this author

Alessandro Schuffner,

Alessandro Schuffner

Centro de Medicina Reprodutiva Conceber, Curitiba (PR), Brasil

Search for more papers by this author

Maria da Graça Bicalho,

Maria da Graça Bicalho

Laboratório de Imunogenética e Histocompatibilidade do Departamento de Genética da Universidade Federal do Paraná (UFPR), Curitiba (PR), Brasil

Search for more papers by this author

Valéria Maria Munhoz Sperandio Roxo,

Corresponding Author

Valéria Maria Munhoz Sperandio Roxo

[email protected]

Laboratório de Imunogenética e Histocompatibilidade do Departamento de Genética da Universidade Federal do Paraná (UFPR), Curitiba (PR), Brasil

Correspondence

Valéria Maria Munhoz Sperandio Roxo, Laboratório de Imunogenética e Histocompatibilidade, Departamento de Genética, Universidade Federal do Paraná, Curitiba –PR, Brazil.

Email: [email protected]

Search for more papers by this author

Georgia Fernanda Gelmini,

Georgia Fernanda Gelmini

Laboratório de Imunogenética e Histocompatibilidade do Departamento de Genética da Universidade Federal do Paraná (UFPR), Curitiba (PR), Brasil

Search for more papers by this author

Cynthia Hernandes Costa,

Cynthia Hernandes Costa

Laboratório de Imunogenética e Histocompatibilidade do Departamento de Genética da Universidade Federal do Paraná (UFPR), Curitiba (PR), Brasil

Search for more papers by this author

Fabiola da Silva Nardi,

Fabiola da Silva Nardi

Laboratório de Imunogenética e Histocompatibilidade do Departamento de Genética da Universidade Federal do Paraná (UFPR), Curitiba (PR), Brasil

Search for more papers by this author

Pryscilla Fanini Wowk,

Pryscilla Fanini Wowk

Laboratório de Imunogenética e Histocompatibilidade do Departamento de Genética da Universidade Federal do Paraná (UFPR), Curitiba (PR), Brasil

Instituto Carlos Chagas, ICC-Fiocruz-PR, Curitiba (PR), Brasil

Search for more papers by this author

Sibelle Botogosque Mattar,

Sibelle Botogosque Mattar

Laboratório de Imunogenética e Histocompatibilidade do Departamento de Genética da Universidade Federal do Paraná (UFPR), Curitiba (PR), Brasil

Instituto Carlos Chagas, ICC-Fiocruz-PR, Curitiba (PR), Brasil

Search for more papers by this author

Alessandro Schuffner,

Alessandro Schuffner

Centro de Medicina Reprodutiva Conceber, Curitiba (PR), Brasil

Search for more papers by this author

Maria da Graça Bicalho,

Maria da Graça Bicalho

Laboratório de Imunogenética e Histocompatibilidade do Departamento de Genética da Universidade Federal do Paraná (UFPR), Curitiba (PR), Brasil

Search for more papers by this author

Valéria Maria Munhoz Sperandio Roxo,

Corresponding Author

Valéria Maria Munhoz Sperandio Roxo

[email protected]

Laboratório de Imunogenética e Histocompatibilidade do Departamento de Genética da Universidade Federal do Paraná (UFPR), Curitiba (PR), Brasil

Correspondence

Valéria Maria Munhoz Sperandio Roxo, Laboratório de Imunogenética e Histocompatibilidade, Departamento de Genética, Universidade Federal do Paraná, Curitiba –PR, Brazil.

Email: [email protected]

Search for more papers by this author

First published: 07 October 2016

https://doi.org/10.1111/aji.12587

Citations: 4

Funding information:

FUNPAR-LIGH

Share a link

Email
Wechat
Bluesky

Abstract

Background

HLA-E products, class Ib human leukocyte antigens, act in the immunology of human reproduction as modulators of the maternal immune system during pregnancy.

Aims

To evaluate HLA-E role in the establishment of a viable pregnancy.

Materials & Methods

HLA-E was genotyped by sequence-based typing (SBT) and analyzed for specific polymorphisms, comparing couples who underwent assisted reproduction treatment (ART) and fertile control couples.

Results

There was a significant difference in HLA-E allele and genotype distributions between ART couples and control couples. The allele HLA-E*01:03 was observed in 63.2% of ART men and in 35.1% of fertile men (P = 0.0032).

Conclusion

These results suggest that HLA-E allelic variants may play a role in the modulation of immune responses in the context of the inability of natural conception and establishment of a viable pregnancy.

References

1Goldberg JM, Falcone T, Attaran M. In vitro fertilization update. Cleve Clin J Med. 2007; 74: 329–338.
10.3949/ccjm.74.5.329
PubMed Web of Science® Google Scholar
2Pauli SA, Berga SL, Shang W, Session DR. Current status of the approach to assisted reproduction. Pediatr Clin North Am. 2009; 56: 467–488.
10.1016/j.pcl.2009.04.001
PubMed Web of Science® Google Scholar
3Moffett-King A. Natural killer cells and pregnancy. Nat Rev Immunol. 2002; 2: 656–663.
10.1038/nri886
CAS PubMed Web of Science® Google Scholar
4Parham P. NK cells and trophoblasts: partners in pregnancy. J Exp Med. 2004; 200: 951–955.
10.1084/jem.20041783
CAS PubMed Web of Science® Google Scholar
5Aagaard-Tillery KM, Silver R, Dalton J. Immunology of normal pregnancy. Semin Fetal Neonatal Med. 2006; 11: 279–295.
10.1016/j.siny.2006.04.003
PubMed Web of Science® Google Scholar
6Ishitani A, Sageshima N, Hatake K. The involvement of HLA-E and -F in pregnancy. J Reprod Immunol. 2006; 69: 101–113.
10.1016/j.jri.2005.10.004
CAS PubMed Web of Science® Google Scholar
7Robinson J, Halliwell JA, Marsh SG. IMGT/HLA and the immuno polymorphism database. Methods Mol Biol. 2014; 1184: 109–121.
10.1007/978-1-4939-1115-8_5
CAS PubMed Google Scholar
8Llano M, Lee N, Navarro F, et al. HLA-E-bound peptides influence recognition by inhibitory and triggering CD94/NKG2 receptors: preferential response to an HLA-G-derived nonamer. Eur J Immunol. 1998; 28: 2854–2863.
10.1002/(SICI)1521-4141(199809)28:09<2854::AID-IMMU2854>3.0.CO;2-W
CAS PubMed Web of Science® Google Scholar
9Ishitani A, Sageshima N, Lee N, et al. Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition. J Immunol. 2003; 171: 1376–1384.
10.4049/jimmunol.171.3.1376
CAS PubMed Web of Science® Google Scholar
10Braud VM, Allan DS, O'Callaghan CA, et al. HLA-E binds to natural killer cell receptors CD94/NKG2A, B and C. Nature. 1998; 391: 795–799.
10.1038/35869
CAS PubMed Web of Science® Google Scholar
11Gömez-Casado E, Martínez-Lasot J, Castro MJ, et al. Detection of HLA-E and -G DNA alleles for population and disease studies. Cell Mol Life Sci. 1999; 56: 356–362.
10.1007/s000180050436
CAS PubMed Web of Science® Google Scholar
12Sullivan LC, Clements CS, Rossjohn J, Brooks AG. The major histocompatibility complex class Ib molecule HLA-E at the interface between innate and adaptive immunity. Tissue Antigens. 2008; 72: 415–424.
10.1111/j.1399-0039.2008.01138.x
CAS PubMed Web of Science® Google Scholar
13dos Santos LC, Tureck LV, Wowk PF, et al. HLA-E polymorphisms in an Afro-descendant Southern Brazilian population. Hum Immunol. 2013; 74: 199–202.
10.1016/j.humimm.2012.10.020
CAS PubMed Web of Science® Google Scholar
14Excoffier L, Laval G, Schneider S. Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evol Bioinform Online. 2005; 1: 47–50.
10.1111/j.1365-294X.2006.03067.x
CAS Web of Science® Google Scholar
15Raymond M, Rousset F. GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered. 1995; 86: 248–249.
10.1093/oxfordjournals.jhered.a111573
Web of Science® Google Scholar
16Ayres M, Ayres-Junior M, Ayres DL, Santos ASD. Bioestat 5.0: statistical Applications in the areas of biological and medical science. Belém, Brasilia: Sociedade Civil Mamirauá, Cnpq; 2007.
Google Scholar
17Rousset F. Genepop'007: a complete re-implementation of the genepop software for windows and linux. Mol Ecol Resour. 2008; 8: 103–106.
10.1111/j.1471-8286.2007.01931.x
PubMed Web of Science® Google Scholar
18Costa CH, Gelmini G F, Wowk PF, et al. HLA-G regulatory haplotypes and implantation outcome in couples who underwent assisted reproduction treatment. Hum Immunol. 2012; 73: 891–897.
10.1016/j.humimm.2012.06.002
CAS PubMed Web of Science® Google Scholar
19Nardi FdS, Slowik R, Wowk PF, et al. Analysis of HLA-G polymorphisms in couples with implantation failure. Am J Reprod Immunol. 2012; 68: 507–514.
10.1111/aji.12001
CAS PubMed Web of Science® Google Scholar
20Vargas RG, Sarturi PR, Mattar SB, et al. Association of HLA-G alleles and 3’ UTR 14 bp haplotypes with recurrent miscarriage in Brazilian couples. Hum Immunol. 2011; 72: 479–485.
10.1016/j.humimm.2011.02.011
CAS PubMed Web of Science® Google Scholar
21Veiga-Castelli LC, Castelli EC, Mendes CT Jr, et al. Non-Classical HLA-E gene variability in Brazilians: a nearly invariable locus surrounded by the most variable genes in the human genome. Tissue Antigens. 2011; 79: 15–24.
10.1111/j.1399-0039.2011.01801.x
CAS PubMed Web of Science® Google Scholar
22Strong RK, Holmes MA, Li P, Braun L, Lee N, Geraghty DE. HLA-E allelic variants. Correlating differential expression, peptide affinities, crystal structures, and thermal stabilities. J Biol Chem. 2003; 278: 5082–5090.
10.1074/jbc.M208268200
CAS PubMed Web of Science® Google Scholar
23Tripathi P, Naik S, Agrawal S. HLA-E and immunobiology of pregnancy. Tissue Antigens. 2006; 67: 207–213.
10.1111/j.1399-0039.2005.00550.x
CAS PubMed Web of Science® Google Scholar
24Kanai T, Fujii T, Keicho N, et al. Polymorphism of human leukocyte antigen-E gene in the Japanese population with or without recurrent abortion. Am J Reprod Immunol. 2001; 45: 168–173.
10.1111/j.8755-8920.2001.450308.x
CAS PubMed Web of Science® Google Scholar
25Steffensen R, Christiansen OB, Bennett EP, Jersild C. HLA-E polymorphism in patients with recurrent spontaneous abortion. Tissue Antigens. 1998; 52: 569–572.
10.1111/j.1399-0039.1998.tb03088.x
CAS PubMed Web of Science® Google Scholar

Citing Literature

Volume76, Issue6

December 2016

Pages 439-442

Is HLA-E a possible genetic marker relevant for natural conception?

Abstract

Background

Aims

Materials & Methods

Results

Conclusion

References

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

Is HLA-E a possible genetic marker relevant for natural conception?

Abstract

Background

Aims

Materials & Methods

Results

Conclusion

References

Citing Literature

References

Related

Information