The Mycobacterium tuberculosis recombinant LprN protein of mce4 operon induces Th-1 type response deleterious to protection in mice
Rashmi Pasricha
Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India
Search for more papers by this authorNeeraj K. Saini
Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India
Search for more papers by this authorNisha Rathor
Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India
Search for more papers by this authorRakesh Pathak
Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India
Search for more papers by this authorRajesh Sinha
Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India
Search for more papers by this authorMandira Varma-Basil
Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India
Search for more papers by this authorKiran Mishra
Department of Pathology, University College of Medical Sciences, Dilshad Garden, Delhi, India
Search for more papers by this authorVani Brahmachari
Dr B.R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi, India
Search for more papers by this authorCorresponding Author
Mridula Bose
Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India
Correspondence
Mridula Bose, Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi-110007, India.
Tel.: +91 11 27666120
fax: +91 11 2766549
e-mail:[email protected]
Search for more papers by this authorRashmi Pasricha
Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India
Search for more papers by this authorNeeraj K. Saini
Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India
Search for more papers by this authorNisha Rathor
Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India
Search for more papers by this authorRakesh Pathak
Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India
Search for more papers by this authorRajesh Sinha
Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India
Search for more papers by this authorMandira Varma-Basil
Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India
Search for more papers by this authorKiran Mishra
Department of Pathology, University College of Medical Sciences, Dilshad Garden, Delhi, India
Search for more papers by this authorVani Brahmachari
Dr B.R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi, India
Search for more papers by this authorCorresponding Author
Mridula Bose
Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India
Correspondence
Mridula Bose, Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi-110007, India.
Tel.: +91 11 27666120
fax: +91 11 2766549
e-mail:[email protected]
Search for more papers by this authorThe recombinant Mycobacterium tuberculosis LprN lipoprotein induced a cell mediated immune response in BALB/c mice as shown by increased T cell proliferation and production of TNF-α and IFN-γ. However, pre-exposure to the LprN protein failed to provide protection in mice after challenge with virulent M. tuberculosis but induced distinct tissue destruction. The LprN lipoprotein may contribute to the virulence of M. tuberculosis.
Abstract
Lipoproteins are known to be effective immunogens and affect both innate and adaptive immunity. The lprN gene of Mycobacterium tuberculosis has been predicted to encode for a putative lipoprotein in silico. Here, we studied its function as an immunogen by in vivo studies in mice. The recombinant LprN protein, expressed and purified in Escherichia coli, triggered a cell-mediated immune response in BALB/c mice. This was observed by significantly higher T-cell proliferation and increased production of TNF-α and IFN-γ cytokines. However, pre-exposure to LprN protein failed to provide protection in mice after challenge with a virulent strain of M. tuberculosis. Histological examination showed an increase in tissue destruction in experimental animals, indicating an immunogenic potential for LprN protein that enhanced the virulence of bacilli.
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