Role of amino acids in rheumatoid arthritis studied by metabolomics
Min He
Analytical BioSciences, LACDR, Leiden University, Leiden, the Netherlands
Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, Leiden, the Netherlands
Search for more papers by this authorAmy C. Harms
Analytical BioSciences, LACDR, Leiden University, Leiden, the Netherlands
Search for more papers by this authorCorresponding Author
Eduard van Wijk
Analytical BioSciences, LACDR, Leiden University, Leiden, the Netherlands
Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, Leiden, the Netherlands
Correspondence: Dr. Eduard van Wijk, Division of Analytical Biosciences, LACDR, Sino-Dutch Centre for Preventive and Personalized Medicine, Leiden University, 2333 CC Leiden, The Netherlands. Email: [email protected]Search for more papers by this authorMei Wang
Analytical BioSciences, LACDR, Leiden University, Leiden, the Netherlands
Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, Leiden, the Netherlands
SU Biomedicine, Zeist, the Netherlands
Search for more papers by this authorRuud Berger
Analytical BioSciences, LACDR, Leiden University, Leiden, the Netherlands
Search for more papers by this authorSlavik Koval
Analytical BioSciences, LACDR, Leiden University, Leiden, the Netherlands
Search for more papers by this authorThomas Hankemeier
Analytical BioSciences, LACDR, Leiden University, Leiden, the Netherlands
Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, Leiden, the Netherlands
Search for more papers by this authorJan van der Greef
Analytical BioSciences, LACDR, Leiden University, Leiden, the Netherlands
Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, Leiden, the Netherlands
TNO, Zeist, the Netherlands
Search for more papers by this authorMin He
Analytical BioSciences, LACDR, Leiden University, Leiden, the Netherlands
Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, Leiden, the Netherlands
Search for more papers by this authorAmy C. Harms
Analytical BioSciences, LACDR, Leiden University, Leiden, the Netherlands
Search for more papers by this authorCorresponding Author
Eduard van Wijk
Analytical BioSciences, LACDR, Leiden University, Leiden, the Netherlands
Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, Leiden, the Netherlands
Correspondence: Dr. Eduard van Wijk, Division of Analytical Biosciences, LACDR, Sino-Dutch Centre for Preventive and Personalized Medicine, Leiden University, 2333 CC Leiden, The Netherlands. Email: [email protected]Search for more papers by this authorMei Wang
Analytical BioSciences, LACDR, Leiden University, Leiden, the Netherlands
Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, Leiden, the Netherlands
SU Biomedicine, Zeist, the Netherlands
Search for more papers by this authorRuud Berger
Analytical BioSciences, LACDR, Leiden University, Leiden, the Netherlands
Search for more papers by this authorSlavik Koval
Analytical BioSciences, LACDR, Leiden University, Leiden, the Netherlands
Search for more papers by this authorThomas Hankemeier
Analytical BioSciences, LACDR, Leiden University, Leiden, the Netherlands
Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, Leiden, the Netherlands
Search for more papers by this authorJan van der Greef
Analytical BioSciences, LACDR, Leiden University, Leiden, the Netherlands
Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, Leiden, the Netherlands
TNO, Zeist, the Netherlands
Search for more papers by this authorAbstract
Background
Rheumatoid arthritis (RA) is a complex, chronic autoimmune disease characterized by various inflammatory symptoms, including joint swelling, joint pain, and both structural and functional joint damage. The most commonly used animal model for studying RA is mice with collagen-induced arthritis (CIA); the wide use of this model is due primarily to many similarities with RA in human patients. Metabolomics is used increasingly in biological studies for diagnosing disease and for predicting and evaluating drug interventions, as a large number of disease-associated metabolites can be analyzed and interpreted from a biological perspective.
Aim
To profile free amino acids and their biogenic metabolites in CIA mice plasma.
Method
Ultra-high-performance liquid chromatography/tandem mass spectrometry coupled with multiple reaction monitoring (MRM) was used for metabolomics study.
Results
Profile of 45 amine metabolites, including free amino acids and their biogenic metabolites in plasma was obtained from CIA mice. We found that the plasma levels of 20 amine metabolites were significantly decreased in the CIA group.
Conclusion
The results suggest that a disordered amine response is linked to RA-associated muscle wasting and energy expenditure.
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