Paper

Analysis of the Effect of Cyclophosphamide and Methotrexate on Chrysomya megacephala (Diptera: Calliphoridae)^†,^‡

Corresponding Author

Ana Letícia Trivia M.Sc.

[email protected]

Museu de Arqueologia e Etnologia Professor Oswaldo Rodrigues Cabral, Universidade Federal de Santa Catarina, Florianópolis, CEP: 88.040-900 Brazil

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Ana Letícia Trivia, M.Sc., Museu de Arqueologia e Etnologia Professor Oswaldo Rodrigues Cabral

Universidade Federal de Santa Catarina,

Florianópolis, CEP: 88.040-900

Brazil

E-mail: [email protected]

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Carlos José de Carvalho Pinto Ph.D.,

Carlos José de Carvalho Pinto Ph.D.

Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina, Florianópolis, CEP: 88.040-900 Brazil

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Ana Letícia Trivia M.Sc.,

Corresponding Author

Ana Letícia Trivia M.Sc.

[email protected]

Museu de Arqueologia e Etnologia Professor Oswaldo Rodrigues Cabral, Universidade Federal de Santa Catarina, Florianópolis, CEP: 88.040-900 Brazil

Additional information and reprint requests

Ana Letícia Trivia, M.Sc., Museu de Arqueologia e Etnologia Professor Oswaldo Rodrigues Cabral

Universidade Federal de Santa Catarina,

Florianópolis, CEP: 88.040-900

Brazil

E-mail: [email protected]

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Carlos José de Carvalho Pinto Ph.D.,

Carlos José de Carvalho Pinto Ph.D.

Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina, Florianópolis, CEP: 88.040-900 Brazil

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First published: 19 January 2018

https://doi.org/10.1111/1556-4029.13740

Citations: 5

^†

Presented at the XXIV Congresso Nacional de Criminalística, October 6, 2017, in Florianópolis, Brazil.

^‡

Financial support provided by the Coordination for the Improvement of Higher Education Personnel (CAPES) of Brazil.

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Abstract

Forensic entomotoxicology investigates the effects of chemical substances in the development of scavenger insects and the reflection on estimating the minimum postmortem interval (PMI). To evaluate the impact of the chemotherapeutic drugs cyclophosphamide (CF) and methotrexate (MTX) on the postembryonic development of Chrysomya megacephala, controlled experiments were performed by rearing the larvae on spiked minced beef with different concentrations of these drugs. The results indicated that CF significantly decreased their developmental rate up to 28 h but had no significant effect on larval and adult sizes, survival rate, and sex ratio, whereas MTX decreased larval and adult sizes, survival rate, and there was a deviation in the expected sex ratio toward females in MTX-exposed larvae but had no significant impact on developmental rate. These negative interference factors should be considered in cases of suspected death of people that have undergone chemotherapy.

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Volume63, Issue5

September 2018

Pages 1413-1418

Analysis of the Effect of Cyclophosphamide and Methotrexate on Chrysomya megacephala (Diptera: Calliphoridae)^†,^‡

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Analysis of the Effect of Cyclophosphamide and Methotrexate on Chrysomya megacephala (Diptera: Calliphoridae)†,‡

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Analysis of the Effect of Cyclophosphamide and Methotrexate on Chrysomya megacephala (Diptera: Calliphoridae)^†,^‡