Journal of Pediatric Gastroenterology and Nutrition

Original Articles: Gastroenterology

Epithelial Electrical Resistance as a Measure of Permeability Changes in Pediatric Duodenal Biopsies

Corresponding Author

Annika Reims

[email protected]

Paediatrics, Sahlgrenska Academy, Göteborg University, Göteborg, Sweden

Address correspondence and reprint requests to Annika Reims, MD, PhD, Department of Paediatrics, Institute for the Health of Women and Children, Queen Silvia Children's Hospital, 416 85 Göteborg, Sweden (e-mail: [email protected]).Search for more papers by this author

Birgitta Strandvik,

Birgitta Strandvik

Paediatrics, Sahlgrenska Academy, Göteborg University, Göteborg, Sweden

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Henrik Sjövall,

Henrik Sjövall

Internal Medicine, Sahlgrenska Academy, Göteborg University, Göteborg, Sweden

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Annika Reims,

Corresponding Author

Annika Reims

[email protected]

Paediatrics, Sahlgrenska Academy, Göteborg University, Göteborg, Sweden

Birgitta Strandvik,

Birgitta Strandvik

Paediatrics, Sahlgrenska Academy, Göteborg University, Göteborg, Sweden

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Henrik Sjövall,

Henrik Sjövall

Internal Medicine, Sahlgrenska Academy, Göteborg University, Göteborg, Sweden

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First published: 01 November 2006

https://doi.org/10.1097/01.mpg.0000232573.33526.f5

Citations: 14

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ABSTRACT

Objectives:

Intestinal permeability measured with medium-sized oral probes is increased in cystic fibrosis (CF) and celiac disease (CD), probably reflecting reduced tight junction resistance. The aim of this study was to evaluate whether square-pulse analysis of duodenal biopsies from children can be used to determine electrical tight junction resistance.

Methods:

Intestinal biopsies from children with different stages of CD and from patients with CF were studied in a modified Ussing chamber. The epithelium was assumed to act as an electrical circuit consisting of a current generator parallel with a resistance and a capacitance. Subepithelial and epithelial resistances were determined by square-pulse analysis, and the generated current was calculated.

Results:

Confirming data using permeability probes, reduced epithelial electrical resistance was found both in patients with CF and CD. Only the CF patients had reduced resting current as well. The secretagogues prostaglandin E₂, cyclic adenosine monophosphate and acetylcholine increased the current in both control biopsies and biopsies with villous atrophy but had no significant effect on epithelial resistance.

Conclusions:

Measurement of electrical resistance in duodenal biopsies can be used as an alternative method of quantifying permeability in pediatric biopsies.

REFERENCES

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Volume43, Issue5

November 2006

Pages 619-623

Epithelial Electrical Resistance as a Measure of Permeability Changes in Pediatric Duodenal Biopsies

ABSTRACT

Objectives:

Methods:

Results:

Conclusions:

REFERENCES

Citing Literature

References

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Epithelial Electrical Resistance as a Measure of Permeability Changes in Pediatric Duodenal Biopsies

ABSTRACT

Objectives:

Methods:

Results:

Conclusions:

REFERENCES

Citing Literature

References

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