Journal of Gastroenterology and Hepatology

Suppression of Helicobacter pylori-induced interleukin-8 production in gastric cancer cell lines by an anti-ulcer drug, geranylgeranylacetone

Corresponding Author

Naoki Yoshimura

The Second Department of Internal Medicine, Chiba University Hospital and

Dr N Yoshimura, Department of Clinical Cell Biology, Graduate School of Medicine (The Second Department of Internal Medicine), Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan. Email: [email protected]Search for more papers by this author

Yasuo Suzuki,

Yasuo Suzuki

The Second Department of Internal Medicine, Chiba University Hospital and

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Yasushi Saito,

Yasushi Saito

Department of Clinical Cell Biology, Graduate School of Medicine, Chiba University, Chiba, Japan

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Naoki Yoshimura,

Corresponding Author

Naoki Yoshimura

The Second Department of Internal Medicine, Chiba University Hospital and

Yasuo Suzuki,

Yasuo Suzuki

The Second Department of Internal Medicine, Chiba University Hospital and

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Yasushi Saito,

Yasushi Saito

Department of Clinical Cell Biology, Graduate School of Medicine, Chiba University, Chiba, Japan

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First published: 01 October 2002

https://doi.org/10.1046/j.1440-1746.2002.02880.x

Citations: 7

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Abstract

Background: Geranylgeranylacetone (GGA) is an antigastritis and anti-ulcer agent, with as yet an unknown mechanism of action. In this study, we investigated the effect of GGA on Helicobacter pylori-induced interleukin (IL)-8 production and IL-8 mRNA expression in KATOIII cells, an established gastric cell line.

Methods: Interleukin-8 production in H. pylori-infected KATOIII cells was measured by using enzyme-linked immunoassay. The cytotoxicity of H. pylori on KATOIII cells was measured by a ⁵¹Cr release assay. The effect of GGA on H. pylori-induced IL-8 mRNA expression was measured by using northern blotting.

Results: Interleukin-8 production increased with time and H. pylori dose; the most significant increase was seen within 6–24 h of coculture with H. pylori. A dose of 0.1 mmol GGA suppressed IL-8 production (P = 0.0077) and inhibited H. pylori-induced IL-8 mRNA expression (P = 0.0019). Furthermore, H. pylori-induced gastric mucosal cell injury associated with IL-8 and neutrophil activation was enhanced by NH₃, and this enhancement was suppressed by GGA (P = 0.0043).

Conclusions: Helicobacter pylori- infected gastric mucosal cells produce IL-8, which can promote neutrophil activation, thus contributing to mucosal tissue injury associated with H. pylori infection. Agents like GGA, which can suppress IL-8 production may have a protective role in the treatment of mucosal tissue damage seen in H. pylori infection.

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Volume17, Issue11

November 2002

Pages 1153-1160

Suppression of Helicobacter pylori-induced interleukin-8 production in gastric cancer cell lines by an anti-ulcer drug, geranylgeranylacetone

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Suppression of Helicobacter pylori-induced interleukin-8 production in gastric cancer cell lines by an anti-ulcer drug, geranylgeranylacetone

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