Variables to predict engraftment of umbilical cord blood into immunodeficient mice: usefulness of the non-obese diabetic–severe combined immunodeficient assay
Karen K. Ballen,
Helen Valinski,
Dale Greiner,
Leonard D. Shultz, Pamela S. Becker,
Chung Cheng Hsieh,
F. Marc Stewart,
Peter J. Quesenberry,
Karen K. Ballen
University of Massachusetts Cancer Center, Worcester, MA, and
Search for more papers by this authorHelen Valinski
University of Massachusetts Cancer Center, Worcester, MA, and
Search for more papers by this authorDale Greiner
University of Massachusetts Cancer Center, Worcester, MA, and
Search for more papers by this authorPamela S. Becker
University of Massachusetts Cancer Center, Worcester, MA, and
Search for more papers by this authorChung Cheng Hsieh
University of Massachusetts Cancer Center, Worcester, MA, and
Search for more papers by this authorF. Marc Stewart
University of Massachusetts Cancer Center, Worcester, MA, and
Search for more papers by this authorPeter J. Quesenberry
University of Massachusetts Cancer Center, Worcester, MA, and
Search for more papers by this authorKaren K. Ballen,
Helen Valinski,
Dale Greiner,
Leonard D. Shultz, Pamela S. Becker,
Chung Cheng Hsieh,
F. Marc Stewart,
Peter J. Quesenberry,
Karen K. Ballen
University of Massachusetts Cancer Center, Worcester, MA, and
Search for more papers by this authorHelen Valinski
University of Massachusetts Cancer Center, Worcester, MA, and
Search for more papers by this authorDale Greiner
University of Massachusetts Cancer Center, Worcester, MA, and
Search for more papers by this authorPamela S. Becker
University of Massachusetts Cancer Center, Worcester, MA, and
Search for more papers by this authorChung Cheng Hsieh
University of Massachusetts Cancer Center, Worcester, MA, and
Search for more papers by this authorF. Marc Stewart
University of Massachusetts Cancer Center, Worcester, MA, and
Search for more papers by this authorPeter J. Quesenberry
University of Massachusetts Cancer Center, Worcester, MA, and
Search for more papers by this author Karen K. Ballen, MD, UMass Memorial Health Care, 55 Lake Avenue North, Worcester, MA 01655, USA. E-mail: [email protected]
Abstract
Umbilical cord blood is an alternative stem cell source for patients without matched family donors. In this study, we examined several parameters that have not been studied in detail – radiation dose, cell dose, age of mice, and maternal and neonatal characteristics of the cord blood donor – that affect engraftment of cord blood in non-obese diabetic–severe combined immunodeficient (NOD–scid) mice. Engraftment, measured using flow cytometry analyses of human CD45+ cells, was highest in 400 cGy-treated mice. Successful engraftment was demonstrated up to 6 months, with a mean engraftment of 31% (range 0–67%) of human cells in recipient bone marrow. Engraftment was skewed to B lymphocytes. The radiation dose of 350 cGy resulted in superior survival of the murine recipients compared with 400 cGy (P = 0.03). The sex of the NOD–scid recipients had a significant effect on survival (female superior to male, P = 0·01), but not on engraftment. There were high levels of variability among different cord units and among animals injected with the same cord unit. This variability may limit the clinical usefulness of the NOD–scid mice as hosts for the quantification of human stem cells.
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