Methods Synthesis of cysteinyl LTs was assessed in patients with AD and healthy volunteers by measuring urinary LTE₄, a useful index of systemic cysteinyl LT synthesis, using liquid chromatography/tandem mass spectrometry.

Results Mean ± SD urinary LTE₄ levels in patients with AD (125 ± 69 pg mg⁻¹ creatinine, n = 20) were significantly higher (P < 0·01) than in healthy volunteers (60 ± 19 pg mg⁻¹ creatinine, n = 17). A significant correlation between urinary LTE₄ and total serum IgE levels in patients with AD was observed (r = 0·643, P < 0·05).

Conclusions Our findings demonstrate an enhanced synthesis of cysteinyl LTs in patients with AD and suggest that cysteinyl LTs are involved in the pathophysiology of AD.

References

1 Jones HE, Inouye JC, McGerity JL & Lewis CW. Atopic disease and serum immunoglobulin-E. Br J Dermatol 1975; 92: 17 – 25.
10.1111/j.1365-2133.1975.tb03028.x
PubMed Web of Science® Google Scholar
2 Hoffman DR, Yamamoto FY, Geller B & Haddad Z. Specific IgE antibodies in atopic eczema. J Allergy Clin Immunol 1975; 55: 256 – 67.
10.1016/0091-6749(75)90145-1
Web of Science® Google Scholar
3 Sampson HA & Albergo R. Comparison of results of prick skin tests, RAST, and double-blind placebo-controlled food challenges in children with atopic dermatitis. J Allergy Clin Immunol 1984; 74: 26 – 33.
10.1016/0091-6749(84)90083-6
PubMed Web of Science® Google Scholar
4 Koro O, Furutani K & Hide M et al. Chemical mediators in atopic dermatitis: involvement of leukotriene B₄ released by a type I allergic reaction in the pathogenesis of atopic dermatitis. J Allergy Clin Immunol 1999; 103: 663 – 70.
10.1016/S0091-6749(99)70240-X
CAS PubMed Web of Science® Google Scholar
5 Brain SD & Williams TJ. Leukotrienes and inflammation. Pharmacol Ther 1990; 46: 57 – 66.
10.1016/0163-7258(90)90035-Z
CAS PubMed Web of Science® Google Scholar
6 Talbot SF, Atkins PC, Goetzl EJ & Zweiman B. Accumulation of leukotriene C₄ and histamine in human allergic skin reactions. J Clin Invest 1985; 76: 650 – 6.
10.1172/JCI112018
CAS PubMed Web of Science® Google Scholar
7 James JM, Kagey-Sobotka A & Sampson HA. Patients with severe atopic dermatitis have activated circulating basophils. J Allergy Clin Immunol 1993; 91: 1155 – 62.
10.1016/0091-6749(93)90318-A
PubMed Web of Science® Google Scholar
8 Westcott JY, Voelkel NF, Jones K & Wenzel SE. Inactivation of leukotriene C₄ in the airways and subsequent urinary leukotriene E₄ excretion in normal and asthmatic subjects. Am Rev Respir Dis 1993; 148: 1244 – 51.
10.1164/ajrccm/148.5.1244
CAS PubMed Web of Science® Google Scholar
9 Fauler J, Neumann CH, Tsikas D & Frölich JC. Enhanced synthesis of cysteinyl leukotrienes in atopic dermatitis. Br J Dermatol 1993; 128: 627 – 30.
10.1111/j.1365-2133.1993.tb00256.x
CAS PubMed Web of Science® Google Scholar
10 Sansom JE, Taylor GW, Dollery CT & Archer CB. Urinary leukotriene E₄ levels in patients with atopic dermatitis. Br J Dermatol 1997; 136: 790 – 1.
10.1046/j.1365-2133.1997.6781625.x
CAS PubMed Web of Science® Google Scholar
11 Mizugaki M, Hishinuma T & Suzuki N. Determination of leukotriene E₄ in human urine using liquid chromatography-tandem mass spectrometry. J Chromatogr B 1999; 729: 279 – 85.
10.1016/S0378-4347(99)00174-7
CAS PubMed Web of Science® Google Scholar
12 Asano K, Lilly CM & O’Donnell WJ et al. Diurnal variation of urinary leukotriene E₄ and histamine excretion rates in normal subjects and patients with mild-to-moderate asthma. J Allergy Clin Immunol 1995; 96: 643 – 51.
10.1016/S0091-6749(95)70263-6
CAS PubMed Web of Science® Google Scholar
13 Stephan V, Kühr R & Urbanek R. Anti-IgE- and complement-induced histamine release from peripheral leukocytes in normals and atopics. Int Arch Allergy Appl Immunol 1989; 90: 326 – 30.
10.1159/000235048
CAS PubMed Web of Science® Google Scholar
14 Bischoff SC, Zwahlen R & Stucki M et al. Basophil histamine release and leukotriene production in response to anti-IgE and anti-IgE receptor antibodies. Int Arch Allergy Appl Immunol 1996; 110: 261 – 71.
10.1159/000237297
CAS PubMed Web of Science® Google Scholar

Citing Literature

Volume144, Issue1

January 2001

Pages 19-23

Increased urinary leukotriene E₄ excretion in patients with atopic dermatitis

Abstract

References

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

Increased urinary leukotriene E4 excretion in patients with atopic dermatitis

Abstract

References

Citing Literature

References

Related

Information

Increased urinary leukotriene E₄ excretion in patients with atopic dermatitis