The Clostridium botulinum GerAB germination protein is located in the inner membrane of spores
François Alberto
INRA UMR A408 Sécurité et Qualité des Produits d'Origine Végétale, Institut National de la Recherche Agronomique, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 9, France
Search for more papers by this authorLucien Botella
INRA UMR A408 Sécurité et Qualité des Produits d'Origine Végétale, Institut National de la Recherche Agronomique, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 9, France
Search for more papers by this authorFréderic Carlin
INRA UMR A408 Sécurité et Qualité des Produits d'Origine Végétale, Institut National de la Recherche Agronomique, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 9, France
Search for more papers by this authorChristophe Nguyen-the
INRA UMR A408 Sécurité et Qualité des Produits d'Origine Végétale, Institut National de la Recherche Agronomique, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 9, France
Search for more papers by this authorCorresponding Author
Véronique Broussolle
INRA UMR A408 Sécurité et Qualité des Produits d'Origine Végétale, Institut National de la Recherche Agronomique, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 9, France
*Corresponding author. Tel.: +33 432 722518; fax: +33 432 722492., E-mail address: [email protected]Search for more papers by this authorFrançois Alberto
INRA UMR A408 Sécurité et Qualité des Produits d'Origine Végétale, Institut National de la Recherche Agronomique, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 9, France
Search for more papers by this authorLucien Botella
INRA UMR A408 Sécurité et Qualité des Produits d'Origine Végétale, Institut National de la Recherche Agronomique, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 9, France
Search for more papers by this authorFréderic Carlin
INRA UMR A408 Sécurité et Qualité des Produits d'Origine Végétale, Institut National de la Recherche Agronomique, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 9, France
Search for more papers by this authorChristophe Nguyen-the
INRA UMR A408 Sécurité et Qualité des Produits d'Origine Végétale, Institut National de la Recherche Agronomique, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 9, France
Search for more papers by this authorCorresponding Author
Véronique Broussolle
INRA UMR A408 Sécurité et Qualité des Produits d'Origine Végétale, Institut National de la Recherche Agronomique, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 9, France
*Corresponding author. Tel.: +33 432 722518; fax: +33 432 722492., E-mail address: [email protected]Search for more papers by this authorEdited by E. Ricca
Abstract
Clostridium botulinum dormant spores germinate in presence of l-alanine via a specific receptor composed of GerAA, GerAB and GerAC proteins. In Bacillus subtilis spores, GerAA and GerAC proteins were located in the inner membrane of the spore. We studied the location of the GerAB protein in C. botulinum spore fractions by Western-blot analysis, using an antipeptidic antibody. The protein GerAB was in vitro translated and used to confirm the specificity of the antibodies. GerAB was not present in a coat and spore outer membrane fraction but was present in a fraction of decoated spores containing inner membrane. These results strongly suggest that the protein GerAB is located in the inner membrane of the spore.
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