Coaggregation between freshwater bacteria within biofilm and planktonic communities
Corresponding Author
A.H. Rickard
School of Pharmacy and Pharmaceutical Sciences, Manchester University, Coupland III Building, Oxford Road, Manchester M13 9PL, UK
*Corresponding author. Tel.: +44 (161) 275 2360; Fax: +44 (161) 275 2396, E-mail address: [email protected]Search for more papers by this authorA.J. McBain
School of Pharmacy and Pharmaceutical Sciences, Manchester University, Coupland III Building, Oxford Road, Manchester M13 9PL, UK
Search for more papers by this authorR.G. Ledder
School of Pharmacy and Pharmaceutical Sciences, Manchester University, Coupland III Building, Oxford Road, Manchester M13 9PL, UK
Search for more papers by this authorP.S. Handley
School of Biological Sciences, Stopford Building, Oxford Road, Manchester M13 9PT, UK
Search for more papers by this authorP. Gilbert
School of Pharmacy and Pharmaceutical Sciences, Manchester University, Coupland III Building, Oxford Road, Manchester M13 9PL, UK
Search for more papers by this authorCorresponding Author
A.H. Rickard
School of Pharmacy and Pharmaceutical Sciences, Manchester University, Coupland III Building, Oxford Road, Manchester M13 9PL, UK
*Corresponding author. Tel.: +44 (161) 275 2360; Fax: +44 (161) 275 2396, E-mail address: [email protected]Search for more papers by this authorA.J. McBain
School of Pharmacy and Pharmaceutical Sciences, Manchester University, Coupland III Building, Oxford Road, Manchester M13 9PL, UK
Search for more papers by this authorR.G. Ledder
School of Pharmacy and Pharmaceutical Sciences, Manchester University, Coupland III Building, Oxford Road, Manchester M13 9PL, UK
Search for more papers by this authorP.S. Handley
School of Biological Sciences, Stopford Building, Oxford Road, Manchester M13 9PT, UK
Search for more papers by this authorP. Gilbert
School of Pharmacy and Pharmaceutical Sciences, Manchester University, Coupland III Building, Oxford Road, Manchester M13 9PL, UK
Search for more papers by this authorAbstract
The coaggregation ability of bacteria isolated from a freshwater biofilm was compared to those derived from the coexisting planktonic population. Twenty-nine morphologically distinct bacterial strains were isolated from a 6-month-old biofilm, established in a glass tank under high-shear conditions, and 15 distinct strains were isolated from the associated re-circulating water. All 44 strains were identified to genus or species level by 16S rDNA sequencing. The 29 biofilm strains belonged to 14 genera and 23.4% of all the possible pair-wise combinations coaggregated. The 15 planktonic strains belonged to seven genera and only 5.8% of all the possible pair-wise combinations coaggregated. Therefore, compared to the planktonic population, a greater proportion of the biofilm strains coaggregated. It is proposed that coaggregation influences biofilm formation and species diversity in freshwater under high shear.
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